A high-throughput pipeline for DNA/RNA/small RNA purification from tissue samples for sequencing

A high-throughput pipeline for DNA/RNA/small RNA purification from tissue samples for sequencing

High-throughput total nucleic acid (TNA) purification methods based on solid-phase reversible immobilization (SPRI) beads produce TNA suitable for both genomic and transcriptomic applications. Even so, small RNA species, including miRNA, bind weakly to SPRI beads under standard TNA purification cond...

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Bibliographic Details
Main Authors: Jing Xu, Pawan K Pandoh, Richard D Corbett, Duane Smailus, Reanne Bowlby, Denise Brooks, Helen McDonald, Simon Haile, Sundeep Chahal, Steve Bilobram, Karen L Mungall, Andrew J Mungall, Robin Coope, Richard A Moore, Yongjun Zhao, Steven JM Jones, Marco A Marra
Format: Article
Language:English
Published: Taylor & Francis Group 2023-08-01
Series:BioTechniques
Subjects:
genomics
miRNA
nucleic acid purification
purification
RNA
RNA-Seq
Online Access:https://www.future-science.com/doi/10.2144/btn-2023-0011
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https://www.future-science.com/doi/10.2144/btn-2023-0011

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