TLC determination of flavonoids from different cultivars of Allium cepa and Allium ascalonicum
This study comprises the optimization and validation of a new TLC method for determination of flavonols in the bulbs of seven cultivars of onions and shallots. Separation was performed on RP-18 plates with the solvent mixture tetrahydrofuran/water/formic acid (40+60+6, V/V/V) as a mobile phase. The...
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| Format: | Article |
| Language: | English |
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Sciendo
2016-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.1515/acph-2016-0038 |
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| author | Pobłocka-Olech Loretta Głód Daniel Żebrowska Maria E. Sznitowska Małgorzata Krauze-Baranowska Miroslawa |
| author_facet | Pobłocka-Olech Loretta Głód Daniel Żebrowska Maria E. Sznitowska Małgorzata Krauze-Baranowska Miroslawa |
| author_sort | Pobłocka-Olech Loretta |
| collection | DOAJ |
| description | This study comprises the optimization and validation of a new TLC method for determination of flavonols in the bulbs of seven cultivars of onions and shallots. Separation was performed on RP-18 plates with the solvent mixture tetrahydrofuran/water/formic acid (40+60+6, V/V/V) as a mobile phase. The method was evaluated for precision, linearity, LOD, LOQ, accuracy and robustness. Chromatographic analysis of the extracts revealed the presence of three main flavonols, quercetin, quercetin-4′-O-glucoside and quercetin-3,4′-O-diglucoside in the majority of analyzed cultivars. The content of flavonols in the analyzed extracts of onion bulbs varied from 123 (‘Exihibition’) to 1079 mg kg-1 fresh mass (fm) (‘Hybing’) in edible parts, and from 1727 (‘Hyline’) to 28949 mg kg-1 fm (‘Red Baron’) in outer scales. The bulbs of two shallot cultivars contained 209 (‘Ambition’) and 523 mg kg-1 fm (‘Matador’) of flavonols in edible parts and 5426 and 8916 mg kg-1 fm in outer scales, respectively. |
| format | Article |
| id | doaj-art-320b7d12c4b24022ad502b0dd8839d5e |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2016-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-320b7d12c4b24022ad502b0dd8839d5e2025-02-02T12:11:59ZengSciendoActa Pharmaceutica1846-95582016-12-0166454355410.1515/acph-2016-0038acph-2016-0038TLC determination of flavonoids from different cultivars of Allium cepa and Allium ascalonicumPobłocka-Olech Loretta0Głód Daniel1Żebrowska Maria E.2Sznitowska Małgorzata3Krauze-Baranowska Miroslawa4Department of Pharmacognosy with Medicinal Plants Garden, Medicine Medical University of Gdańsk 80-416 Gdańsk, PolandDepartment of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk 80-416 Gdańsk, PolandDepartment of Pharmaceutical Technology, Faculty of Pharmacy with Subfaculty of Laboratory Medicine Medical University of Gdańsk 80-416 Gdańsk, PolandDepartment of Pharmaceutical Technology Faculty of Pharmacy with Subfaculty of Laboratory Medicine Medical University of Gdańsk 80-416 Gdańsk, PolandDepartment of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk 80-416 Gdańsk, PolandThis study comprises the optimization and validation of a new TLC method for determination of flavonols in the bulbs of seven cultivars of onions and shallots. Separation was performed on RP-18 plates with the solvent mixture tetrahydrofuran/water/formic acid (40+60+6, V/V/V) as a mobile phase. The method was evaluated for precision, linearity, LOD, LOQ, accuracy and robustness. Chromatographic analysis of the extracts revealed the presence of three main flavonols, quercetin, quercetin-4′-O-glucoside and quercetin-3,4′-O-diglucoside in the majority of analyzed cultivars. The content of flavonols in the analyzed extracts of onion bulbs varied from 123 (‘Exihibition’) to 1079 mg kg-1 fresh mass (fm) (‘Hybing’) in edible parts, and from 1727 (‘Hyline’) to 28949 mg kg-1 fm (‘Red Baron’) in outer scales. The bulbs of two shallot cultivars contained 209 (‘Ambition’) and 523 mg kg-1 fm (‘Matador’) of flavonols in edible parts and 5426 and 8916 mg kg-1 fm in outer scales, respectively.https://doi.org/10.1515/acph-2016-0038allium cepaallium ascalonicumcultivar varietiesflavonolstlcquantitative analysis |
| spellingShingle | Pobłocka-Olech Loretta Głód Daniel Żebrowska Maria E. Sznitowska Małgorzata Krauze-Baranowska Miroslawa TLC determination of flavonoids from different cultivars of Allium cepa and Allium ascalonicum Acta Pharmaceutica allium cepa allium ascalonicum cultivar varieties flavonols tlc quantitative analysis |
| title | TLC determination of flavonoids from different cultivars of Allium cepa and Allium ascalonicum |
| title_full | TLC determination of flavonoids from different cultivars of Allium cepa and Allium ascalonicum |
| title_fullStr | TLC determination of flavonoids from different cultivars of Allium cepa and Allium ascalonicum |
| title_full_unstemmed | TLC determination of flavonoids from different cultivars of Allium cepa and Allium ascalonicum |
| title_short | TLC determination of flavonoids from different cultivars of Allium cepa and Allium ascalonicum |
| title_sort | tlc determination of flavonoids from different cultivars of allium cepa and allium ascalonicum |
| topic | allium cepa allium ascalonicum cultivar varieties flavonols tlc quantitative analysis |
| url | https://doi.org/10.1515/acph-2016-0038 |
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