Rapid detection of Pseudomonas aeruginosa by glycerol one-pot RAA/CRISPR-Cas12a method

Rapid detection of Pseudomonas aeruginosa by glycerol one-pot RAA/CRISPR-Cas12a method

Pseudomonas aeruginosa (PA), an opportunistic pathogen commonly responsible for hospital-acquired infections, poses significant threats to human health. To enable rapid and reliable PA detection while effectively mitigating aerosol contamination risks inherent in conventional methods. We developed a...

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Bibliographic Details
Main Authors: Lijian Wei, Shihua Luo, Weijie Zhou, Baoyan Ren, Miao Li, Lina Liang, Xuebin Li, Guijiang Wei
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Chemistry
Subjects:
recombinase-aided amplification
glycerol one-pot CRISPR/Cas12a method
lateral flow chromatography strip
rapid detection
Pseudomonas aeruginosa
Online Access:https://www.frontiersin.org/articles/10.3389/fchem.2025.1654270/full
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https://www.frontiersin.org/articles/10.3389/fchem.2025.1654270/full

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