CRISPR/Cas9 ribonucleoprotein mediated DNA-free genome editing in larch

Here, a DNA-free genetic editing approach is presented for larch by delivering ribonucleoprotein complexes (RNPs) of CRISPR/Cas9 through particle bombardment. The detailed procedure encompasses creating a transgenic system via particle bombardment for the transformation of embryogenic callus, valida...

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Bibliographic Details
Main Authors: Miaomiao Ma, Chan Zhang, Lijing Yu, Jingli Yang, Chenghao Li
Format: Article
Language:English
Published: Maximum Academic Press 2024-01-01
Series:Forestry Research
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Online Access:https://www.maxapress.com/article/doi/10.48130/forres-0024-0033
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