VEGFR-2 Expression in Glioblastoma Multiforme Depends on Inflammatory Tumor Microenvironment
Glioblastoma multiforme (GBM) is one of the most angiogenic tumors. However, antiangiogenic therapy has not shown significant clinical efficacy. The aim of our study was to evaluate the impact of inflammatory tumor microenvironment on the expression of vascular endothelial growth factor receptor 2 (...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/385030 |
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| author | Jana Jaal Marju Kase Ave Minajeva Mikk Saretok Aidi Adamson Jelizaveta Junninen Tõnis Metsaots Tõnu Jõgi Madis Joonsalu Markus Vardja Toomas Asser |
| author_facet | Jana Jaal Marju Kase Ave Minajeva Mikk Saretok Aidi Adamson Jelizaveta Junninen Tõnis Metsaots Tõnu Jõgi Madis Joonsalu Markus Vardja Toomas Asser |
| author_sort | Jana Jaal |
| collection | DOAJ |
| description | Glioblastoma multiforme (GBM) is one of the most angiogenic tumors. However, antiangiogenic therapy has not shown significant clinical efficacy. The aim of our study was to evaluate the impact of inflammatory tumor microenvironment on the expression of vascular endothelial growth factor receptor 2 (VEGFR-2). Surgically excised primary GBM tissues were histologically examined for overall extent of inflammation (score 1–3). After immunohistochemistry, the tissue expression of ICAM-1 (optical density), the number of VEGFR-2 positive (VEGFR-2+) blood vessels (per microscopic field), and the endothelial staining intensity of VEGFR-2 (score 0–3) were determined. In GBM, the extent of inflammation was 1.9 ± 0.7 (group mean ± SD). Mean optical density of inflammatory mediator ICAM-1 was 57.0 ± 27.1 (pixel values). The number of VEGFR-2+ blood vessels and endothelial VEGFR-2 staining intensity were 6.2 ± 2.4 and 1.2 ± 0.8, respectively. A positive association was found between endothelial VEGFR-2 staining intensity and the extent of inflammation (p=0.005). Moreover, VEGFR-2 staining intensity correlated with the expression level of ICAM-1 (p=0.026). The expression of VEGFR-2, one of the main targets of antiangiogenic therapy, depends on GBM microenvironment. Higher endothelial VEGFR-2 levels were seen in the presence of more pronounced inflammation. Target dependence on inflammatory tumor microenvironment has to be taken into consideration when treatment approaches that block VEGFR-2 signaling are designed. |
| format | Article |
| id | doaj-art-3144e164afa94a27a6efb7f0baf4ecba |
| institution | Kabale University |
| issn | 2090-8040 2042-0099 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Inflammation |
| spelling | doaj-art-3144e164afa94a27a6efb7f0baf4ecba2025-02-03T01:00:04ZengWileyInternational Journal of Inflammation2090-80402042-00992015-01-01201510.1155/2015/385030385030VEGFR-2 Expression in Glioblastoma Multiforme Depends on Inflammatory Tumor MicroenvironmentJana Jaal0Marju Kase1Ave Minajeva2Mikk Saretok3Aidi Adamson4Jelizaveta Junninen5Tõnis Metsaots6Tõnu Jõgi7Madis Joonsalu8Markus Vardja9Toomas Asser10Hematology and Oncology Clinic, Department of Radiotherapy and Oncological Therapy, Tartu University Hospital, 51003 Tartu, EstoniaHematology and Oncology Clinic, Department of Radiotherapy and Oncological Therapy, Tartu University Hospital, 51003 Tartu, EstoniaFaculty of Medicine, University of Tartu, 50411 Tartu, EstoniaFaculty of Medicine, University of Tartu, 50411 Tartu, EstoniaFaculty of Medicine, University of Tartu, 50411 Tartu, EstoniaFaculty of Medicine, University of Tartu, 50411 Tartu, EstoniaFaculty of Medicine, University of Tartu, 50411 Tartu, EstoniaHematology and Oncology Clinic, Department of Radiotherapy and Oncological Therapy, Tartu University Hospital, 51003 Tartu, EstoniaHematology and Oncology Clinic, Department of Radiotherapy and Oncological Therapy, Tartu University Hospital, 51003 Tartu, EstoniaHematology and Oncology Clinic, Department of Radiotherapy and Oncological Therapy, Tartu University Hospital, 51003 Tartu, EstoniaFaculty of Medicine, University of Tartu, 50411 Tartu, EstoniaGlioblastoma multiforme (GBM) is one of the most angiogenic tumors. However, antiangiogenic therapy has not shown significant clinical efficacy. The aim of our study was to evaluate the impact of inflammatory tumor microenvironment on the expression of vascular endothelial growth factor receptor 2 (VEGFR-2). Surgically excised primary GBM tissues were histologically examined for overall extent of inflammation (score 1–3). After immunohistochemistry, the tissue expression of ICAM-1 (optical density), the number of VEGFR-2 positive (VEGFR-2+) blood vessels (per microscopic field), and the endothelial staining intensity of VEGFR-2 (score 0–3) were determined. In GBM, the extent of inflammation was 1.9 ± 0.7 (group mean ± SD). Mean optical density of inflammatory mediator ICAM-1 was 57.0 ± 27.1 (pixel values). The number of VEGFR-2+ blood vessels and endothelial VEGFR-2 staining intensity were 6.2 ± 2.4 and 1.2 ± 0.8, respectively. A positive association was found between endothelial VEGFR-2 staining intensity and the extent of inflammation (p=0.005). Moreover, VEGFR-2 staining intensity correlated with the expression level of ICAM-1 (p=0.026). The expression of VEGFR-2, one of the main targets of antiangiogenic therapy, depends on GBM microenvironment. Higher endothelial VEGFR-2 levels were seen in the presence of more pronounced inflammation. Target dependence on inflammatory tumor microenvironment has to be taken into consideration when treatment approaches that block VEGFR-2 signaling are designed.http://dx.doi.org/10.1155/2015/385030 |
| spellingShingle | Jana Jaal Marju Kase Ave Minajeva Mikk Saretok Aidi Adamson Jelizaveta Junninen Tõnis Metsaots Tõnu Jõgi Madis Joonsalu Markus Vardja Toomas Asser VEGFR-2 Expression in Glioblastoma Multiforme Depends on Inflammatory Tumor Microenvironment International Journal of Inflammation |
| title | VEGFR-2 Expression in Glioblastoma Multiforme Depends on Inflammatory Tumor Microenvironment |
| title_full | VEGFR-2 Expression in Glioblastoma Multiforme Depends on Inflammatory Tumor Microenvironment |
| title_fullStr | VEGFR-2 Expression in Glioblastoma Multiforme Depends on Inflammatory Tumor Microenvironment |
| title_full_unstemmed | VEGFR-2 Expression in Glioblastoma Multiforme Depends on Inflammatory Tumor Microenvironment |
| title_short | VEGFR-2 Expression in Glioblastoma Multiforme Depends on Inflammatory Tumor Microenvironment |
| title_sort | vegfr 2 expression in glioblastoma multiforme depends on inflammatory tumor microenvironment |
| url | http://dx.doi.org/10.1155/2015/385030 |
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