APPLICATION OF SOME GROWTH REGULATORS IN THE INITATION AND DIFFERENTIATION OF CALLUS TO REGENERATION OF DWARF MARIGOLD (Tagetes patula cv. Nana) plants 1- CALLUS INITATION
The current study applied many of growth regulators involved: 6-benzyladenine (BA) alone and interacted with the two auxin each of ; naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The callus was obtained from different explants (leaves, stem, and roots).The results showed...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
College of Agriculture
2009-12-01
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| Series: | Mesopotamia Journal of Agriculture |
| Online Access: | https://magrj.mosuljournals.com/article_27514_ce9bae81123bbdc9eb1f363ade4630e6.pdf |
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| Summary: | The current study applied many of growth regulators involved: 6-benzyladenine (BA) alone and interacted with the two auxin each of ; naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The callus was obtained from different explants (leaves, stem, and roots).The results showed that leaves were the best explants for callus initiation specially by application of BA with 2,4-D in solidified MS medium which gave 100% initiation percentage of callus in all interaction was used ,this interaction of BA and 2,4-D also was active in initiation of stem callus (85%) . The interaction of BA with NAA also gave higher initiation percentage of leaves and roots callus with height weight of leaves callus reached 9.847g/explants after 30 days of growth on MS medium supplemented with 5mgL-1 of BA and 0.1mgL-1of NAA. BA alone by the used concentrations was able to induce the callus in all explants , and showed 100% initiation percentage of leaves and roots callus at 4 and 5 mgL-1 concentrations and prompt ,on MS medium with5 mgL-1 BA, the growth of stem and roots callus reached to 4.78 and 3.87gm respectively after 30 days of culture . |
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| ISSN: | 1815-316X 2224-9796 |