Physicochemical, structural characterization, and antioxidant activities of chondroitin sulfate from Oreochromis niloticus bones
In this study, chondroitin sulfate was extracted from Oreochromis niloticus bones (OCS) and isolated to three fractions (OCS-1, OCS-2, and OCS-3). The physicochemical properties and structure characterization including monosaccharide, disaccharide compositions, molecular weight (Mw) of OCS were dete...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002464 |
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| author | Jun Yang Mingyue Shen Ting Wu Xianxiang Chen Huiliang Wen Jianhua Xie |
| author_facet | Jun Yang Mingyue Shen Ting Wu Xianxiang Chen Huiliang Wen Jianhua Xie |
| author_sort | Jun Yang |
| collection | DOAJ |
| description | In this study, chondroitin sulfate was extracted from Oreochromis niloticus bones (OCS) and isolated to three fractions (OCS-1, OCS-2, and OCS-3). The physicochemical properties and structure characterization including monosaccharide, disaccharide compositions, molecular weight (Mw) of OCS were determined by HPAEC, HPLC-SAX, HPGPC, FT-IR spectra, and 1D/2D NMR. Moreover, their thermal properties, crystalline structure, and microstructure were also analyzed. Results showed that their Mw were between 10 kDa and 50 kDa. CS-6 was the predominant disaccharide unit in four OCS, and the CS-4/CS-6 ratios were close to CS from shark cartilage. Besides, the results of antioxidant activity showed that different fractions of OCS had a distinct DPPH radical, hydroxyl radical, and ABTS+ radical scavenging activity. OCS-1 has the highest scavenging activities in DPPH and hydroxyl radical compared with other fractions, which showed a higher medicinal value. Those findings may lay some theoretical basis for the potential application development of OCS. |
| format | Article |
| id | doaj-art-2f77a424104f451b9c053c8087f0d7bf |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-2f77a424104f451b9c053c8087f0d7bf2025-02-03T04:05:21ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112411021108Physicochemical, structural characterization, and antioxidant activities of chondroitin sulfate from Oreochromis niloticus bonesJun Yang0Mingyue Shen1Ting Wu2Xianxiang Chen3Huiliang Wen4Jianhua Xie5State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaCorresponding author at: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaIn this study, chondroitin sulfate was extracted from Oreochromis niloticus bones (OCS) and isolated to three fractions (OCS-1, OCS-2, and OCS-3). The physicochemical properties and structure characterization including monosaccharide, disaccharide compositions, molecular weight (Mw) of OCS were determined by HPAEC, HPLC-SAX, HPGPC, FT-IR spectra, and 1D/2D NMR. Moreover, their thermal properties, crystalline structure, and microstructure were also analyzed. Results showed that their Mw were between 10 kDa and 50 kDa. CS-6 was the predominant disaccharide unit in four OCS, and the CS-4/CS-6 ratios were close to CS from shark cartilage. Besides, the results of antioxidant activity showed that different fractions of OCS had a distinct DPPH radical, hydroxyl radical, and ABTS+ radical scavenging activity. OCS-1 has the highest scavenging activities in DPPH and hydroxyl radical compared with other fractions, which showed a higher medicinal value. Those findings may lay some theoretical basis for the potential application development of OCS.http://www.sciencedirect.com/science/article/pii/S2213453022002464Oreochromis niloticus by-productsChondroitin sulfateStructural characterizationAntioxidant |
| spellingShingle | Jun Yang Mingyue Shen Ting Wu Xianxiang Chen Huiliang Wen Jianhua Xie Physicochemical, structural characterization, and antioxidant activities of chondroitin sulfate from Oreochromis niloticus bones Food Science and Human Wellness Oreochromis niloticus by-products Chondroitin sulfate Structural characterization Antioxidant |
| title | Physicochemical, structural characterization, and antioxidant activities of chondroitin sulfate from Oreochromis niloticus bones |
| title_full | Physicochemical, structural characterization, and antioxidant activities of chondroitin sulfate from Oreochromis niloticus bones |
| title_fullStr | Physicochemical, structural characterization, and antioxidant activities of chondroitin sulfate from Oreochromis niloticus bones |
| title_full_unstemmed | Physicochemical, structural characterization, and antioxidant activities of chondroitin sulfate from Oreochromis niloticus bones |
| title_short | Physicochemical, structural characterization, and antioxidant activities of chondroitin sulfate from Oreochromis niloticus bones |
| title_sort | physicochemical structural characterization and antioxidant activities of chondroitin sulfate from oreochromis niloticus bones |
| topic | Oreochromis niloticus by-products Chondroitin sulfate Structural characterization Antioxidant |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002464 |
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