Comparative Analysis of Embryonic Development and Antioxidant Stress Response in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) with Different Ploidy Levels
Triploid fish sometimes grow faster and larger than diploids, making them valuable in aquaculture. Their underdeveloped gonads redirect energy from reproduction to muscle growth. This study compared embryonic development and antioxidant responses between allotriploid and hybrid diploid grouper (<...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Fishes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2410-3888/10/1/4 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588471524392960 |
|---|---|
| author | Haizhan Tang Yang Li Meng Zhang Xin Fan Jian Luo Xin Wen |
| author_facet | Haizhan Tang Yang Li Meng Zhang Xin Fan Jian Luo Xin Wen |
| author_sort | Haizhan Tang |
| collection | DOAJ |
| description | Triploid fish sometimes grow faster and larger than diploids, making them valuable in aquaculture. Their underdeveloped gonads redirect energy from reproduction to muscle growth. This study compared embryonic development and antioxidant responses between allotriploid and hybrid diploid grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) after cold shock, aiming to support large-scale allotriploid production. The diploid group had a 99.08% fertilization rate and 82.87% hatching rate, while the triploid group had lower rates (95.71% fertilization, 39.63% hatching, 11.52% deformity). Triploids developed later, with higher incidences of abnormalities and mortality, especially in the gastrula and neuro-embryo stages. Triploid larvae exhibited shortened tails, abnormal yolk sacs, and impaired swimming. Flow cytometry showed triploids that had 1.55 times more DNA and larger red blood cells. Triploids had lower midgut trypsin and lipase levels than diploids (<i>p</i> < 0.05) but similar glucose, liver malondialdehyde, and total cholesterol levels (<i>p</i> > 0.05). However, they had higher liver and spleen lactate dehydrogenase, catalase, and alkaline phosphatase, along with lower spleen malondialdehyde and liver superoxide dismutase (<i>p</i> < 0.05). These findings offer insights into reducing mortality in allotriploid grouper aquaculture, aiding large-scale production efforts. |
| format | Article |
| id | doaj-art-2f5ea086c14441f58730cf82974e40d0 |
| institution | Kabale University |
| issn | 2410-3888 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj-art-2f5ea086c14441f58730cf82974e40d02025-01-24T13:32:23ZengMDPI AGFishes2410-38882024-12-01101410.3390/fishes10010004Comparative Analysis of Embryonic Development and Antioxidant Stress Response in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) with Different Ploidy LevelsHaizhan Tang0Yang Li1Meng Zhang2Xin Fan3Jian Luo4Xin Wen5Hainan Aquaculture Breeding Engineering Research Center, Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Academician Team Innovation Center, Hainan University, Haikou 570228, ChinaHainan Aquaculture Breeding Engineering Research Center, Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Academician Team Innovation Center, Hainan University, Haikou 570228, ChinaHainan Aquaculture Breeding Engineering Research Center, Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Academician Team Innovation Center, Hainan University, Haikou 570228, ChinaHainan Aquaculture Breeding Engineering Research Center, Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Academician Team Innovation Center, Hainan University, Haikou 570228, ChinaHainan Aquaculture Breeding Engineering Research Center, Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Academician Team Innovation Center, Hainan University, Haikou 570228, ChinaHainan Aquaculture Breeding Engineering Research Center, Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Academician Team Innovation Center, Hainan University, Haikou 570228, ChinaTriploid fish sometimes grow faster and larger than diploids, making them valuable in aquaculture. Their underdeveloped gonads redirect energy from reproduction to muscle growth. This study compared embryonic development and antioxidant responses between allotriploid and hybrid diploid grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) after cold shock, aiming to support large-scale allotriploid production. The diploid group had a 99.08% fertilization rate and 82.87% hatching rate, while the triploid group had lower rates (95.71% fertilization, 39.63% hatching, 11.52% deformity). Triploids developed later, with higher incidences of abnormalities and mortality, especially in the gastrula and neuro-embryo stages. Triploid larvae exhibited shortened tails, abnormal yolk sacs, and impaired swimming. Flow cytometry showed triploids that had 1.55 times more DNA and larger red blood cells. Triploids had lower midgut trypsin and lipase levels than diploids (<i>p</i> < 0.05) but similar glucose, liver malondialdehyde, and total cholesterol levels (<i>p</i> > 0.05). However, they had higher liver and spleen lactate dehydrogenase, catalase, and alkaline phosphatase, along with lower spleen malondialdehyde and liver superoxide dismutase (<i>p</i> < 0.05). These findings offer insights into reducing mortality in allotriploid grouper aquaculture, aiding large-scale production efforts.https://www.mdpi.com/2410-3888/10/1/4polyploidyEpinephelinaeembryogenesisstressantioxidant |
| spellingShingle | Haizhan Tang Yang Li Meng Zhang Xin Fan Jian Luo Xin Wen Comparative Analysis of Embryonic Development and Antioxidant Stress Response in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) with Different Ploidy Levels Fishes polyploidy Epinephelinae embryogenesis stress antioxidant |
| title | Comparative Analysis of Embryonic Development and Antioxidant Stress Response in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) with Different Ploidy Levels |
| title_full | Comparative Analysis of Embryonic Development and Antioxidant Stress Response in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) with Different Ploidy Levels |
| title_fullStr | Comparative Analysis of Embryonic Development and Antioxidant Stress Response in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) with Different Ploidy Levels |
| title_full_unstemmed | Comparative Analysis of Embryonic Development and Antioxidant Stress Response in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) with Different Ploidy Levels |
| title_short | Comparative Analysis of Embryonic Development and Antioxidant Stress Response in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) with Different Ploidy Levels |
| title_sort | comparative analysis of embryonic development and antioxidant stress response in hybrid grouper i epinephelus fuscoguttatus i ♀ i e lanceolatus i ♂ with different ploidy levels |
| topic | polyploidy Epinephelinae embryogenesis stress antioxidant |
| url | https://www.mdpi.com/2410-3888/10/1/4 |
| work_keys_str_mv | AT haizhantang comparativeanalysisofembryonicdevelopmentandantioxidantstressresponseinhybridgrouperiepinephelusfuscoguttatusiielanceolatusiwithdifferentploidylevels AT yangli comparativeanalysisofembryonicdevelopmentandantioxidantstressresponseinhybridgrouperiepinephelusfuscoguttatusiielanceolatusiwithdifferentploidylevels AT mengzhang comparativeanalysisofembryonicdevelopmentandantioxidantstressresponseinhybridgrouperiepinephelusfuscoguttatusiielanceolatusiwithdifferentploidylevels AT xinfan comparativeanalysisofembryonicdevelopmentandantioxidantstressresponseinhybridgrouperiepinephelusfuscoguttatusiielanceolatusiwithdifferentploidylevels AT jianluo comparativeanalysisofembryonicdevelopmentandantioxidantstressresponseinhybridgrouperiepinephelusfuscoguttatusiielanceolatusiwithdifferentploidylevels AT xinwen comparativeanalysisofembryonicdevelopmentandantioxidantstressresponseinhybridgrouperiepinephelusfuscoguttatusiielanceolatusiwithdifferentploidylevels |