Sulodexide alleviates high glucose-induced cardiac microvascular endothelial cell injury in rat by activating the AMPK/SIRT1 pathway
Objective To study the effect and mechanism of sulodexide (SDX) on the oxidative stress and apoptosis of cardiac microvascular endothelial cells (CMEC) in rats under high glucose (HG). Methods CMEC of rats were isolated and cultured, and randomly divided into control group, HG group and HG+SDX group...
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Editorial Office of Journal of Guangxi Medical University
2024-07-01
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| Series: | Guangxi Yike Daxue xuebao |
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| Online Access: | https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.07.009 |
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| author | GAO Yanli REN Yingang WANG Jie KANG Li |
| author_facet | GAO Yanli REN Yingang WANG Jie KANG Li |
| author_sort | GAO Yanli |
| collection | DOAJ |
| description | Objective To study the effect and mechanism of sulodexide (SDX) on the oxidative stress and apoptosis of cardiac microvascular endothelial cells (CMEC) in rats under high glucose (HG). Methods CMEC of rats were isolated and cultured, and randomly divided into control group, HG group and HG+SDX group. Cell survival rates were measured by cell counting kit-8 (CCK-8) assay. DCFH-DA was used as a fluorescence probe to determine the fluorescence intensity of DCF in each group to determine the level of intracellular reactive oxygen species (ROS). The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the supernatant were compared. The apoptosis rate was detected by flow cytometry and TUNEL assay. The expression of AMPK, phosphorylated (p-) AMPK and SIRT1 proteins was detected by western blotting. Results Compared with the control group, the HG group showed decreased cell survival rate, increased cell supernatant level of MDA, reduced SOD activity, elevated ROS level, increased apoptosis rate, and decreased expression of pAMPK、SIRT1 proteins (all P＜0.05). Compared with the HG group, the survival rate of CMEC cells in the HG+ SDX was significantly increased, the production of MDA and ROS was decreased, the activity of SOD was increased, the apoptosis rate of CMEC was decreased, and the expression of p-AMPK and SIRT1 proteins was significantly increased (all P＜0.05). Conclusion SDX may alleviate HG-induced oxidative stress and apoptosis of CMEC by activating AMPK/SIRT1 signaling pathway, thus playing a cardiovascular protective role. |
| format | Article |
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| institution | DOAJ |
| issn | 1005-930X |
| language | zho |
| publishDate | 2024-07-01 |
| publisher | Editorial Office of Journal of Guangxi Medical University |
| record_format | Article |
| series | Guangxi Yike Daxue xuebao |
| spelling | doaj-art-2e6d84dbd4f643d8a8f2bfc7ebc5698b2025-08-20T02:57:28ZzhoEditorial Office of Journal of Guangxi Medical UniversityGuangxi Yike Daxue xuebao1005-930X2024-07-014171003100810.16190/j.cnki.45-1211/r.2024.07.00920240709Sulodexide alleviates high glucose-induced cardiac microvascular endothelial cell injury in rat by activating the AMPK/SIRT1 pathwayGAO Yanli0REN Yingang1WANG Jie2KANG Li3Department of Geriatric Medicine, Tangdu Hospital of Air Force Military Medical University, Xi'an 710038, ChinaDepartment of Geriatric Medicine, Tangdu Hospital of Air Force Military Medical University, Xi'an 710038, ChinaDepartment of Geriatric Medicine, Tangdu Hospital of Air Force Military Medical University, Xi'an 710038, ChinaDepartment of Geriatric Medicine, Tangdu Hospital of Air Force Military Medical University, Xi'an 710038, ChinaObjective To study the effect and mechanism of sulodexide (SDX) on the oxidative stress and apoptosis of cardiac microvascular endothelial cells (CMEC) in rats under high glucose (HG). Methods CMEC of rats were isolated and cultured, and randomly divided into control group, HG group and HG+SDX group. Cell survival rates were measured by cell counting kit-8 (CCK-8) assay. DCFH-DA was used as a fluorescence probe to determine the fluorescence intensity of DCF in each group to determine the level of intracellular reactive oxygen species (ROS). The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the supernatant were compared. The apoptosis rate was detected by flow cytometry and TUNEL assay. The expression of AMPK, phosphorylated (p-) AMPK and SIRT1 proteins was detected by western blotting. Results Compared with the control group, the HG group showed decreased cell survival rate, increased cell supernatant level of MDA, reduced SOD activity, elevated ROS level, increased apoptosis rate, and decreased expression of pAMPK、SIRT1 proteins (all P＜0.05). Compared with the HG group, the survival rate of CMEC cells in the HG+ SDX was significantly increased, the production of MDA and ROS was decreased, the activity of SOD was increased, the apoptosis rate of CMEC was decreased, and the expression of p-AMPK and SIRT1 proteins was significantly increased (all P＜0.05). Conclusion SDX may alleviate HG-induced oxidative stress and apoptosis of CMEC by activating AMPK/SIRT1 signaling pathway, thus playing a cardiovascular protective role.https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.07.009sulodexidehigh glucosecardiac microvascular endothelial celloxidative stressapoptosis |
| spellingShingle | GAO Yanli REN Yingang WANG Jie KANG Li Sulodexide alleviates high glucose-induced cardiac microvascular endothelial cell injury in rat by activating the AMPK/SIRT1 pathway Guangxi Yike Daxue xuebao sulodexide high glucose cardiac microvascular endothelial cell oxidative stress apoptosis |
| title | Sulodexide alleviates high glucose-induced cardiac microvascular endothelial cell injury in rat by activating the AMPK/SIRT1 pathway |
| title_full | Sulodexide alleviates high glucose-induced cardiac microvascular endothelial cell injury in rat by activating the AMPK/SIRT1 pathway |
| title_fullStr | Sulodexide alleviates high glucose-induced cardiac microvascular endothelial cell injury in rat by activating the AMPK/SIRT1 pathway |
| title_full_unstemmed | Sulodexide alleviates high glucose-induced cardiac microvascular endothelial cell injury in rat by activating the AMPK/SIRT1 pathway |
| title_short | Sulodexide alleviates high glucose-induced cardiac microvascular endothelial cell injury in rat by activating the AMPK/SIRT1 pathway |
| title_sort | sulodexide alleviates high glucose induced cardiac microvascular endothelial cell injury in rat by activating the ampk sirt1 pathway |
| topic | sulodexide high glucose cardiac microvascular endothelial cell oxidative stress apoptosis |
| url | https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.07.009 |
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