Anti-diabetic potential of apigenin, luteolin, and baicalein via partially activating PI3K/Akt/Glut-4 signaling pathways in insulin-resistant HepG2 cells
Dietary flavonoids are abundant in natural plants and possess multiple pharmacological and nutritional activities. In this study, apigenin, luteolin, and baicalein were chosen to evaluate their anti-diabetic effect in high-glucose and dexamethasone induced insulin-resistant (IR) HepG2 cells. All fla...
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Tsinghua University Press
2023-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000733 |
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| author | Lingchao Miao Haolin Zhang Meng Sam Cheong Ruting Zhong Paula Garcia-Oliveira Miguel A. Prieto Ka-Wing Cheng Mingfu Wang Hui Cao Shaoping Nie Jesus Simal-Gandara Wai San Cheang Jianbo Xiao |
| author_facet | Lingchao Miao Haolin Zhang Meng Sam Cheong Ruting Zhong Paula Garcia-Oliveira Miguel A. Prieto Ka-Wing Cheng Mingfu Wang Hui Cao Shaoping Nie Jesus Simal-Gandara Wai San Cheang Jianbo Xiao |
| author_sort | Lingchao Miao |
| collection | DOAJ |
| description | Dietary flavonoids are abundant in natural plants and possess multiple pharmacological and nutritional activities. In this study, apigenin, luteolin, and baicalein were chosen to evaluate their anti-diabetic effect in high-glucose and dexamethasone induced insulin-resistant (IR) HepG2 cells. All flavonoids improves the glucose consumption and glycogen synthesis abilities in IR-HepG2 cells via activating glucose transporter protein 4 (GLUT4) and phosphor-glycogen synthase kinase (GSK-3β). These flavonoids significantly inhibited the production of reactive oxygen species (ROS) and advanced glycation end-products (AGEs), which were closely related to the suppression of the phosphorylation form of NF-κB and P65. The expression levels of insulin receptor substrate-1 (IRS-1), insulin receptor substrate-2 (IRS-2) and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) pathway in IR-HepG2 cells were all partially activated by the flavonoids, with variable effects. Furthermore, the intracellular metabolic conditions of the flavonoids were also evaluated. |
| format | Article |
| id | doaj-art-2d3d504e5ec74d1ebea668eaa2c94ac9 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-2d3d504e5ec74d1ebea668eaa2c94ac92025-02-03T02:42:14ZengTsinghua University PressFood Science and Human Wellness2213-45302023-11-0112619912000Anti-diabetic potential of apigenin, luteolin, and baicalein via partially activating PI3K/Akt/Glut-4 signaling pathways in insulin-resistant HepG2 cellsLingchao Miao0Haolin Zhang1Meng Sam Cheong2Ruting Zhong3Paula Garcia-Oliveira4Miguel A. Prieto5Ka-Wing Cheng6Mingfu Wang7Hui Cao8Shaoping Nie9Jesus Simal-Gandara10Wai San Cheang11Jianbo Xiao12Institute of Chinese Medical Sciences, University of Macau, Macau 999078, ChinaInstitute of Chinese Medical Sciences, University of Macau, Macau 999078, ChinaInstitute of Chinese Medical Sciences, University of Macau, Macau 999078, ChinaInstitute of Chinese Medical Sciences, University of Macau, Macau 999078, ChinaDepartment of Analytical Chemistry and Food Science, Faculty of Food Science and Technology, University of Vigo-Ourense Campus, Ourense E-32004, SpainDepartment of Analytical Chemistry and Food Science, Faculty of Food Science and Technology, University of Vigo-Ourense Campus, Ourense E-32004, SpainInstitute for Advanced Study, Shenzhen University, Shenzhen 508060, ChinaInstitute for Advanced Study, Shenzhen University, Shenzhen 508060, China; School of Biological Sciences, The University of Hong Kong, Hong Kong 999077, ChinaDepartment of Analytical Chemistry and Food Science, Faculty of Food Science and Technology, University of Vigo-Ourense Campus, Ourense E-32004, Spain; College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, ChinaState Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, Nanchang 330047, ChinaDepartment of Analytical Chemistry and Food Science, Faculty of Food Science and Technology, University of Vigo-Ourense Campus, Ourense E-32004, SpainInstitute of Chinese Medical Sciences, University of Macau, Macau 999078, China; Corresponding authors.Department of Analytical Chemistry and Food Science, Faculty of Food Science and Technology, University of Vigo-Ourense Campus, Ourense E-32004, Spain; International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang 212013, China; Corresponding authors.Dietary flavonoids are abundant in natural plants and possess multiple pharmacological and nutritional activities. In this study, apigenin, luteolin, and baicalein were chosen to evaluate their anti-diabetic effect in high-glucose and dexamethasone induced insulin-resistant (IR) HepG2 cells. All flavonoids improves the glucose consumption and glycogen synthesis abilities in IR-HepG2 cells via activating glucose transporter protein 4 (GLUT4) and phosphor-glycogen synthase kinase (GSK-3β). These flavonoids significantly inhibited the production of reactive oxygen species (ROS) and advanced glycation end-products (AGEs), which were closely related to the suppression of the phosphorylation form of NF-κB and P65. The expression levels of insulin receptor substrate-1 (IRS-1), insulin receptor substrate-2 (IRS-2) and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) pathway in IR-HepG2 cells were all partially activated by the flavonoids, with variable effects. Furthermore, the intracellular metabolic conditions of the flavonoids were also evaluated.http://www.sciencedirect.com/science/article/pii/S2213453023000733ApigeninLuteolinBaicaleinInsulin-resistant HepG2 cellsSignaling pathwayreactive oxygen species (ROS) |
| spellingShingle | Lingchao Miao Haolin Zhang Meng Sam Cheong Ruting Zhong Paula Garcia-Oliveira Miguel A. Prieto Ka-Wing Cheng Mingfu Wang Hui Cao Shaoping Nie Jesus Simal-Gandara Wai San Cheang Jianbo Xiao Anti-diabetic potential of apigenin, luteolin, and baicalein via partially activating PI3K/Akt/Glut-4 signaling pathways in insulin-resistant HepG2 cells Food Science and Human Wellness Apigenin Luteolin Baicalein Insulin-resistant HepG2 cells Signaling pathway reactive oxygen species (ROS) |
| title | Anti-diabetic potential of apigenin, luteolin, and baicalein via partially activating PI3K/Akt/Glut-4 signaling pathways in insulin-resistant HepG2 cells |
| title_full | Anti-diabetic potential of apigenin, luteolin, and baicalein via partially activating PI3K/Akt/Glut-4 signaling pathways in insulin-resistant HepG2 cells |
| title_fullStr | Anti-diabetic potential of apigenin, luteolin, and baicalein via partially activating PI3K/Akt/Glut-4 signaling pathways in insulin-resistant HepG2 cells |
| title_full_unstemmed | Anti-diabetic potential of apigenin, luteolin, and baicalein via partially activating PI3K/Akt/Glut-4 signaling pathways in insulin-resistant HepG2 cells |
| title_short | Anti-diabetic potential of apigenin, luteolin, and baicalein via partially activating PI3K/Akt/Glut-4 signaling pathways in insulin-resistant HepG2 cells |
| title_sort | anti diabetic potential of apigenin luteolin and baicalein via partially activating pi3k akt glut 4 signaling pathways in insulin resistant hepg2 cells |
| topic | Apigenin Luteolin Baicalein Insulin-resistant HepG2 cells Signaling pathway reactive oxygen species (ROS) |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000733 |
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