STORAGE OF MESENCHYMAL STEM CELLS IN DIFFERENT SOLUTIONS AND TEMPERATURES
Objective: Mesenchymal Stem Cells (MSCs) have the ability to self-renew and differentiate into various cell types. Their use in regenerative medicine is widespread today. However, for successful clinical application, the viability and potency of MSCs must be maintained during pretransplant preparati...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Istanbul University Press
2022-02-01
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| Series: | Sabiad |
| Subjects: | |
| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/85B97F6980FD468A812B0CDC39F0E08E |
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| Summary: | Objective: Mesenchymal Stem Cells (MSCs) have the ability to self-renew and differentiate into various cell types. Their use in regenerative medicine is widespread today. However, for successful clinical application, the viability and potency of MSCs must be maintained during pretransplant preparation and transportation. The aim of this study (an area which has not been adequately researched), is to contribute to the literature on storage conditions, which is important for maintaining the effectiveness of MSCs. Material and Method: The viability of MSCs and CD73, CD90, CD105, CD19 surface antigens were evaluated in short-term in vitro storage in Phosphate Buffered Saline (PBS) and Serum Physiological solution at 4°C and room temperature (24°C). Conclusion: Although MSCs are similar in terms of viability and surface antigens in PBS and SF solution at 4°C and 24°C, higher results were obtained in cells kept in PBS at 4°C. Further research can be conducted by analyzing the differentiation, aging and proliferation capacities of MSCs under different environmental conditions. |
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| ISSN: | 2651-4060 |