Differences in the Antigens of Helicobacter pylori Strains Influence on the Innate Immune Response in the In Vitro Experiments
The immune response to Helicobacter pylori importantly determines the pathogenesis of infection as well as the success of antibiotic eradication of the bacteria. Strains of H. pylori were gathered from 14 patients who failed to eradicate H. pylori infection with antibiotics—therapy resistant strains...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/287531 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832564673033011200 |
|---|---|
| author | Miha Skvarc Andreja Natasa Kopitar Janko Kos Natasa Obermajer Bojan Tepes |
| author_facet | Miha Skvarc Andreja Natasa Kopitar Janko Kos Natasa Obermajer Bojan Tepes |
| author_sort | Miha Skvarc |
| collection | DOAJ |
| description | The immune response to Helicobacter pylori importantly determines the pathogenesis of infection as well as the success of antibiotic eradication of the bacteria. Strains of H. pylori were gathered from 14 patients who failed to eradicate H. pylori infection with antibiotics—therapy resistant strains (TRS)—or from patients who were able to eradicate H. pylori infection—therapy susceptible strains (TSS). The THP-1 cells were stimulated with H. pylori antigens. Cathepsin X expression on THP-1 cells and concentration of cytokines in the supernatant of THP-1 cells were measured with a flow cytometer.
TSS H. pylori antigens increased the proportion of cathepsin X positive cells compared to TRS H. pylori antigens. TSS H. pylori antigens induced higher secretion of IL-12 and IL-6 compared to TRS H. pylori antigens (P<0.001; 0.02). Polymyxin B, a lipid A inhibitor, lowered the secretion of IL-12 and IL-6 in TRS and TSS.
We demonstrated a H. pylori strain-dependent cathepsin X and cytokine expression that can be associated with H. pylori resistance to eradication due to lack of effective immune response. Differences in lipid A of H. pylori might have an influence on the insufficient immune response, especially on phagocytosis. |
| format | Article |
| id | doaj-art-2cdec8a457c24e5bae57163aaf8fc102 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-2cdec8a457c24e5bae57163aaf8fc1022025-02-03T01:10:22ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/287531287531Differences in the Antigens of Helicobacter pylori Strains Influence on the Innate Immune Response in the In Vitro ExperimentsMiha Skvarc0Andreja Natasa Kopitar1Janko Kos2Natasa Obermajer3Bojan Tepes4Institute of Microbiology and Immunology, Medical Faculty Ljubljana, University of Ljubljana, Zaloska 4, 1000 Ljubljana, SloveniaInstitute of Microbiology and Immunology, Medical Faculty Ljubljana, University of Ljubljana, Zaloska 4, 1000 Ljubljana, SloveniaDepartment of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000 Ljubljana, SloveniaDepartment of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000 Ljubljana, SloveniaAM-Diagnostic Centre Rogaska, Prvomajska ulica 29A, 3250 Rogaska Slatina, SloveniaThe immune response to Helicobacter pylori importantly determines the pathogenesis of infection as well as the success of antibiotic eradication of the bacteria. Strains of H. pylori were gathered from 14 patients who failed to eradicate H. pylori infection with antibiotics—therapy resistant strains (TRS)—or from patients who were able to eradicate H. pylori infection—therapy susceptible strains (TSS). The THP-1 cells were stimulated with H. pylori antigens. Cathepsin X expression on THP-1 cells and concentration of cytokines in the supernatant of THP-1 cells were measured with a flow cytometer. TSS H. pylori antigens increased the proportion of cathepsin X positive cells compared to TRS H. pylori antigens. TSS H. pylori antigens induced higher secretion of IL-12 and IL-6 compared to TRS H. pylori antigens (P<0.001; 0.02). Polymyxin B, a lipid A inhibitor, lowered the secretion of IL-12 and IL-6 in TRS and TSS. We demonstrated a H. pylori strain-dependent cathepsin X and cytokine expression that can be associated with H. pylori resistance to eradication due to lack of effective immune response. Differences in lipid A of H. pylori might have an influence on the insufficient immune response, especially on phagocytosis.http://dx.doi.org/10.1155/2014/287531 |
| spellingShingle | Miha Skvarc Andreja Natasa Kopitar Janko Kos Natasa Obermajer Bojan Tepes Differences in the Antigens of Helicobacter pylori Strains Influence on the Innate Immune Response in the In Vitro Experiments Mediators of Inflammation |
| title | Differences in the Antigens of Helicobacter pylori Strains Influence on the Innate Immune Response in the In Vitro Experiments |
| title_full | Differences in the Antigens of Helicobacter pylori Strains Influence on the Innate Immune Response in the In Vitro Experiments |
| title_fullStr | Differences in the Antigens of Helicobacter pylori Strains Influence on the Innate Immune Response in the In Vitro Experiments |
| title_full_unstemmed | Differences in the Antigens of Helicobacter pylori Strains Influence on the Innate Immune Response in the In Vitro Experiments |
| title_short | Differences in the Antigens of Helicobacter pylori Strains Influence on the Innate Immune Response in the In Vitro Experiments |
| title_sort | differences in the antigens of helicobacter pylori strains influence on the innate immune response in the in vitro experiments |
| url | http://dx.doi.org/10.1155/2014/287531 |
| work_keys_str_mv | AT mihaskvarc differencesintheantigensofhelicobacterpyloristrainsinfluenceontheinnateimmuneresponseintheinvitroexperiments AT andrejanatasakopitar differencesintheantigensofhelicobacterpyloristrainsinfluenceontheinnateimmuneresponseintheinvitroexperiments AT jankokos differencesintheantigensofhelicobacterpyloristrainsinfluenceontheinnateimmuneresponseintheinvitroexperiments AT natasaobermajer differencesintheantigensofhelicobacterpyloristrainsinfluenceontheinnateimmuneresponseintheinvitroexperiments AT bojantepes differencesintheantigensofhelicobacterpyloristrainsinfluenceontheinnateimmuneresponseintheinvitroexperiments |