Analysis of the Reliability of Feather Sections for Corticosterone Measurement in Pekin Ducks
The aim of this study was to identify the feather section, among the whole feather, rachis, and vane, with the highest reliability for corticosterone measurement in 10 Pekin ducks aged 42 days. In total, 60 samples (i.e., 20 per section) were collected and each section was analyzed in duplicate. Cor...
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2025-01-01
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| author | Se-Jin Lim Chan Ho Kim Ka Young Yang Woo Do Lee Su Mi Kim Yang-Ho Choi Jung Hwan Jeon |
| author_facet | Se-Jin Lim Chan Ho Kim Ka Young Yang Woo Do Lee Su Mi Kim Yang-Ho Choi Jung Hwan Jeon |
| author_sort | Se-Jin Lim |
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| description | The aim of this study was to identify the feather section, among the whole feather, rachis, and vane, with the highest reliability for corticosterone measurement in 10 Pekin ducks aged 42 days. In total, 60 samples (i.e., 20 per section) were collected and each section was analyzed in duplicate. Corticosterone levels were measured using ELISA and statistical analyses were performed using ANOVA in SAS 9.2, and the intra-class correlation coefficient (ICC) was tested using IBM SPSS. The level in the whole feather (12.55 ± 4.41 pg/mg) was significantly lower (<i>p</i> < 0.001) than that in the rachis (18.12 ± 5.70 pg/mg). No significant differences were observed between the rachis and the vane (20.40 ± 3.04 pg/mg). ANOVA results confirmed substantial hormonal variability depending on the feather part analyzed. The ICCs for the whole feather, rachis, and vane were 0.923, 0.876, and 0.004, respectively. The vane section exhibited the highest concentration and lowest variance, whereas the whole feather exhibited the highest ICC. Although the whole feather had the lowest concentration, it exhibited greater consistency. Further research is necessary to improve the feather corticosterone analysis method for more accurate analysis. In conclusion, the whole feather provides the most reliable measure of corticosterone concentration among the three parts. |
| format | Article |
| id | doaj-art-2c206a01c19f4e85843d530d15a7d938 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-2c206a01c19f4e85843d530d15a7d9382025-01-24T13:17:43ZengMDPI AGAnimals2076-26152025-01-0115213810.3390/ani15020138Analysis of the Reliability of Feather Sections for Corticosterone Measurement in Pekin DucksSe-Jin Lim0Chan Ho Kim1Ka Young Yang2Woo Do Lee3Su Mi Kim4Yang-Ho Choi5Jung Hwan Jeon6Animal Welfare Research Team, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Republic of KoreaAnimal Welfare Research Team, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Republic of KoreaAnimal Welfare Research Team, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Republic of KoreaAnimal Welfare Research Team, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Republic of KoreaAnimal Welfare Research Team, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Republic of KoreaDepartment of Animal Science, Gyeongsang National University, Jinju 52828, Republic of KoreaAnimal Welfare Research Team, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Republic of KoreaThe aim of this study was to identify the feather section, among the whole feather, rachis, and vane, with the highest reliability for corticosterone measurement in 10 Pekin ducks aged 42 days. In total, 60 samples (i.e., 20 per section) were collected and each section was analyzed in duplicate. Corticosterone levels were measured using ELISA and statistical analyses were performed using ANOVA in SAS 9.2, and the intra-class correlation coefficient (ICC) was tested using IBM SPSS. The level in the whole feather (12.55 ± 4.41 pg/mg) was significantly lower (<i>p</i> < 0.001) than that in the rachis (18.12 ± 5.70 pg/mg). No significant differences were observed between the rachis and the vane (20.40 ± 3.04 pg/mg). ANOVA results confirmed substantial hormonal variability depending on the feather part analyzed. The ICCs for the whole feather, rachis, and vane were 0.923, 0.876, and 0.004, respectively. The vane section exhibited the highest concentration and lowest variance, whereas the whole feather exhibited the highest ICC. Although the whole feather had the lowest concentration, it exhibited greater consistency. Further research is necessary to improve the feather corticosterone analysis method for more accurate analysis. In conclusion, the whole feather provides the most reliable measure of corticosterone concentration among the three parts.https://www.mdpi.com/2076-2615/15/2/138feather analysiscorticosteroneICCmethodologyanimal welfarePekin ducks |
| spellingShingle | Se-Jin Lim Chan Ho Kim Ka Young Yang Woo Do Lee Su Mi Kim Yang-Ho Choi Jung Hwan Jeon Analysis of the Reliability of Feather Sections for Corticosterone Measurement in Pekin Ducks Animals feather analysis corticosterone ICC methodology animal welfare Pekin ducks |
| title | Analysis of the Reliability of Feather Sections for Corticosterone Measurement in Pekin Ducks |
| title_full | Analysis of the Reliability of Feather Sections for Corticosterone Measurement in Pekin Ducks |
| title_fullStr | Analysis of the Reliability of Feather Sections for Corticosterone Measurement in Pekin Ducks |
| title_full_unstemmed | Analysis of the Reliability of Feather Sections for Corticosterone Measurement in Pekin Ducks |
| title_short | Analysis of the Reliability of Feather Sections for Corticosterone Measurement in Pekin Ducks |
| title_sort | analysis of the reliability of feather sections for corticosterone measurement in pekin ducks |
| topic | feather analysis corticosterone ICC methodology animal welfare Pekin ducks |
| url | https://www.mdpi.com/2076-2615/15/2/138 |
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