Enhanced MICP for Soil Improvement and Heavy Metal Remediation: Insights from Landfill Leachate-Derived Ureolytic Bacterial Consortium
This study investigates the potential of microbial-induced calcium carbonate precipitation (MICP) for soil stabilization and heavy metal immobilization, utilizing landfill leachate-derived ureolytic consortium. Experimental conditions identified yeast extract-based media as most effective for bacter...
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2025-01-01
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| author | Armstrong Ighodalo Omoregie Fock-Kui Kan Hazlami Fikri Basri Muhammad Oliver Ensor Silini Adharsh Rajasekar |
| author_facet | Armstrong Ighodalo Omoregie Fock-Kui Kan Hazlami Fikri Basri Muhammad Oliver Ensor Silini Adharsh Rajasekar |
| author_sort | Armstrong Ighodalo Omoregie |
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| description | This study investigates the potential of microbial-induced calcium carbonate precipitation (MICP) for soil stabilization and heavy metal immobilization, utilizing landfill leachate-derived ureolytic consortium. Experimental conditions identified yeast extract-based media as most effective for bacterial growth, urease activity, and calcite formation compared to nutrient broth and brown sugar media. Optimal MICP conditions, at pH 8–9 and 30 °C, supported the most efficient biomineralization. The process facilitated the removal of Cd<sup>2+</sup> (99.10%) and Ni<sup>2+</sup> (78.33%) while producing stable calcite crystals that enhanced soil strength. Thermal analyses (thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC)) confirmed the successful production of CaCO<sub>3</sub> and its role in improving soil stability. DSC analysis revealed endothermic and exothermic peaks, including a significant exothermic peak at 444 °C, corresponding to the thermal decomposition of CaCO<sub>3</sub> into CO<sub>2</sub> and CaO, confirming calcite formation. TGA results showed steady weight loss, consistent with the breakdown of CaCO<sub>3</sub>, supporting the formation of stable carbonates. The MICP treatment significantly increased soil strength, with the highest surface strength observed at 440 psi, correlating with the highest CaCO<sub>3</sub> content (18.83%). These findings underscore the effectiveness of MICP in soil stabilization, pollutant removal, and improving geotechnical properties. |
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| institution | Kabale University |
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| series | Microorganisms |
| spelling | doaj-art-2bd2ec56fe794b64812c6b2f32c7affa2025-01-24T13:42:56ZengMDPI AGMicroorganisms2076-26072025-01-0113117410.3390/microorganisms13010174Enhanced MICP for Soil Improvement and Heavy Metal Remediation: Insights from Landfill Leachate-Derived Ureolytic Bacterial ConsortiumArmstrong Ighodalo Omoregie0Fock-Kui Kan1Hazlami Fikri Basri2Muhammad Oliver Ensor Silini3Adharsh Rajasekar4Research Centre for Borneo Regionalism and Conservation, School of Built Environment, University of Technology Sarawak, No. 1 Jalan University, Sibu 96000, MalaysiaResearch Centre for Borneo Regionalism and Conservation, School of Built Environment, University of Technology Sarawak, No. 1 Jalan University, Sibu 96000, MalaysiaDepartment of Water and Environmental Engineering, Faculty of Civil Engineering, Universiti Teknologi Malaysia, Johor Bahru 81310, MalaysiaResearch Centre for Borneo Regionalism and Conservation, School of Built Environment, University of Technology Sarawak, No. 1 Jalan University, Sibu 96000, MalaysiaKey Laboratory of Meteorological Disaster, Ministry of Education (KLME)/Joint International Research Laboratory of Climate and Environmental Change (ILCEC)/Collaborative Innovation Centre on Forecast and Evaluation of Meteorological Disasters (CIC-FEMD), Nanjing University of Information Science & Technology, Nanjing 210044, ChinaThis study investigates the potential of microbial-induced calcium carbonate precipitation (MICP) for soil stabilization and heavy metal immobilization, utilizing landfill leachate-derived ureolytic consortium. Experimental conditions identified yeast extract-based media as most effective for bacterial growth, urease activity, and calcite formation compared to nutrient broth and brown sugar media. Optimal MICP conditions, at pH 8–9 and 30 °C, supported the most efficient biomineralization. The process facilitated the removal of Cd<sup>2+</sup> (99.10%) and Ni<sup>2+</sup> (78.33%) while producing stable calcite crystals that enhanced soil strength. Thermal analyses (thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC)) confirmed the successful production of CaCO<sub>3</sub> and its role in improving soil stability. DSC analysis revealed endothermic and exothermic peaks, including a significant exothermic peak at 444 °C, corresponding to the thermal decomposition of CaCO<sub>3</sub> into CO<sub>2</sub> and CaO, confirming calcite formation. TGA results showed steady weight loss, consistent with the breakdown of CaCO<sub>3</sub>, supporting the formation of stable carbonates. The MICP treatment significantly increased soil strength, with the highest surface strength observed at 440 psi, correlating with the highest CaCO<sub>3</sub> content (18.83%). These findings underscore the effectiveness of MICP in soil stabilization, pollutant removal, and improving geotechnical properties.https://www.mdpi.com/2076-2607/13/1/174microbial-induced calcium carbonate precipitationsoil stabilizationheavy metal removalureolytic bacterialandfill leachate |
| spellingShingle | Armstrong Ighodalo Omoregie Fock-Kui Kan Hazlami Fikri Basri Muhammad Oliver Ensor Silini Adharsh Rajasekar Enhanced MICP for Soil Improvement and Heavy Metal Remediation: Insights from Landfill Leachate-Derived Ureolytic Bacterial Consortium Microorganisms microbial-induced calcium carbonate precipitation soil stabilization heavy metal removal ureolytic bacteria landfill leachate |
| title | Enhanced MICP for Soil Improvement and Heavy Metal Remediation: Insights from Landfill Leachate-Derived Ureolytic Bacterial Consortium |
| title_full | Enhanced MICP for Soil Improvement and Heavy Metal Remediation: Insights from Landfill Leachate-Derived Ureolytic Bacterial Consortium |
| title_fullStr | Enhanced MICP for Soil Improvement and Heavy Metal Remediation: Insights from Landfill Leachate-Derived Ureolytic Bacterial Consortium |
| title_full_unstemmed | Enhanced MICP for Soil Improvement and Heavy Metal Remediation: Insights from Landfill Leachate-Derived Ureolytic Bacterial Consortium |
| title_short | Enhanced MICP for Soil Improvement and Heavy Metal Remediation: Insights from Landfill Leachate-Derived Ureolytic Bacterial Consortium |
| title_sort | enhanced micp for soil improvement and heavy metal remediation insights from landfill leachate derived ureolytic bacterial consortium |
| topic | microbial-induced calcium carbonate precipitation soil stabilization heavy metal removal ureolytic bacteria landfill leachate |
| url | https://www.mdpi.com/2076-2607/13/1/174 |
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