Changes in PD-1 expression on T lymphocyte subsets and related immune indicators before and after definitive chemoradiotherapy for esophageal squamous cell carcinoma
Objective This study aimed to observe the dynamic changes in the expression of T lymphocytes, natural killer (NK) cells, and PD-1 in patients with first-diagnosed esophageal squamous cell carcinoma (ESCC) before and after chemoradiotherapy (CRT) and evaluate the impact of PD-1 expression in peripher...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Annals of Medicine |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/07853890.2024.2445190 |
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| author | Xueling Shi Hongyu Zhao Jiaqi Yu Peng Cai Shixiang Zhou Ning Yang Duojie Li |
| author_facet | Xueling Shi Hongyu Zhao Jiaqi Yu Peng Cai Shixiang Zhou Ning Yang Duojie Li |
| author_sort | Xueling Shi |
| collection | DOAJ |
| description | Objective This study aimed to observe the dynamic changes in the expression of T lymphocytes, natural killer (NK) cells, and PD-1 in patients with first-diagnosed esophageal squamous cell carcinoma (ESCC) before and after chemoradiotherapy (CRT) and evaluate the impact of PD-1 expression in peripheral blood on the short-term outcome of patients with ESCC.Patients and Methods Seventy-three patients with ESCC who were treated with definitive CRT were enrolled. Before and after CRT, flow cytometry was used to detect thePD-1 expression in the peripheral blood and related immune indicators. Peripheral blood from 10 healthy individuals was used as control.Results The percentages of CD3+ (p = 0.018), CD4+ (p < 0.001), and CD8+ T cells (p < 0.001); NK cells (p = 0.009); and the CD4+/CD8+ ratio (p < 0.001), as well as PD-1+CD3+ (p < 0.001), PD-1+CD4+ (p < 0.001), and PD-1+CD8+ (p < 0.001) T cells, before CRT significantly differed from those in the post-CRT group. The percentages of PD-1+CD8+ T cells differed significantly between the radiotherapy alone and CRT groups (p < 0.05). PD-1 expression in CD3+, CD4+, and CD8+ T cells significantly decreased in patients achieving overall response rate (all p < 0.05). Compared with those in the incomplete response group, PD-1+CD8+ T cells significantly decreased in the CR group (p < 0.05).Conclusion CRT aggravated immunosuppression and increased PD-1 expression in T lymphocyte subsets in patients with ESCC, possibly related to the radiation field. PD-1 expression in T lymphocyte subsets can predict short-term outcomes in patients and provide a theoretical basis for the sequential application of PD-1 immunosuppressants after radiotherapy and chemotherapy. |
| format | Article |
| id | doaj-art-2b453b666bf84381b0ad90e9a82c8f1c |
| institution | Kabale University |
| issn | 0785-3890 1365-2060 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Annals of Medicine |
| spelling | doaj-art-2b453b666bf84381b0ad90e9a82c8f1c2025-01-24T14:18:00ZengTaylor & Francis GroupAnnals of Medicine0785-38901365-20602025-12-0157110.1080/07853890.2024.2445190Changes in PD-1 expression on T lymphocyte subsets and related immune indicators before and after definitive chemoradiotherapy for esophageal squamous cell carcinomaXueling Shi0Hongyu Zhao1Jiaqi Yu2Peng Cai3Shixiang Zhou4Ning Yang5Duojie Li6Department of Radiotherapy, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, ChinaDepartment of Radiotherapy, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, ChinaDepartment of Radiotherapy, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, ChinaDepartment of Radiotherapy, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, ChinaDepartment of Radiotherapy, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, ChinaDepartment of Radiotherapy, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, ChinaDepartment of Radiotherapy, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, ChinaObjective This study aimed to observe the dynamic changes in the expression of T lymphocytes, natural killer (NK) cells, and PD-1 in patients with first-diagnosed esophageal squamous cell carcinoma (ESCC) before and after chemoradiotherapy (CRT) and evaluate the impact of PD-1 expression in peripheral blood on the short-term outcome of patients with ESCC.Patients and Methods Seventy-three patients with ESCC who were treated with definitive CRT were enrolled. Before and after CRT, flow cytometry was used to detect thePD-1 expression in the peripheral blood and related immune indicators. Peripheral blood from 10 healthy individuals was used as control.Results The percentages of CD3+ (p = 0.018), CD4+ (p < 0.001), and CD8+ T cells (p < 0.001); NK cells (p = 0.009); and the CD4+/CD8+ ratio (p < 0.001), as well as PD-1+CD3+ (p < 0.001), PD-1+CD4+ (p < 0.001), and PD-1+CD8+ (p < 0.001) T cells, before CRT significantly differed from those in the post-CRT group. The percentages of PD-1+CD8+ T cells differed significantly between the radiotherapy alone and CRT groups (p < 0.05). PD-1 expression in CD3+, CD4+, and CD8+ T cells significantly decreased in patients achieving overall response rate (all p < 0.05). Compared with those in the incomplete response group, PD-1+CD8+ T cells significantly decreased in the CR group (p < 0.05).Conclusion CRT aggravated immunosuppression and increased PD-1 expression in T lymphocyte subsets in patients with ESCC, possibly related to the radiation field. PD-1 expression in T lymphocyte subsets can predict short-term outcomes in patients and provide a theoretical basis for the sequential application of PD-1 immunosuppressants after radiotherapy and chemotherapy.https://www.tandfonline.com/doi/10.1080/07853890.2024.2445190PD-1T lymphocyte subsetsNK cellsimmunotherapychemoradiotherapyesophageal squamous cell carcinoma |
| spellingShingle | Xueling Shi Hongyu Zhao Jiaqi Yu Peng Cai Shixiang Zhou Ning Yang Duojie Li Changes in PD-1 expression on T lymphocyte subsets and related immune indicators before and after definitive chemoradiotherapy for esophageal squamous cell carcinoma Annals of Medicine PD-1 T lymphocyte subsets NK cells immunotherapy chemoradiotherapy esophageal squamous cell carcinoma |
| title | Changes in PD-1 expression on T lymphocyte subsets and related immune indicators before and after definitive chemoradiotherapy for esophageal squamous cell carcinoma |
| title_full | Changes in PD-1 expression on T lymphocyte subsets and related immune indicators before and after definitive chemoradiotherapy for esophageal squamous cell carcinoma |
| title_fullStr | Changes in PD-1 expression on T lymphocyte subsets and related immune indicators before and after definitive chemoradiotherapy for esophageal squamous cell carcinoma |
| title_full_unstemmed | Changes in PD-1 expression on T lymphocyte subsets and related immune indicators before and after definitive chemoradiotherapy for esophageal squamous cell carcinoma |
| title_short | Changes in PD-1 expression on T lymphocyte subsets and related immune indicators before and after definitive chemoradiotherapy for esophageal squamous cell carcinoma |
| title_sort | changes in pd 1 expression on t lymphocyte subsets and related immune indicators before and after definitive chemoradiotherapy for esophageal squamous cell carcinoma |
| topic | PD-1 T lymphocyte subsets NK cells immunotherapy chemoradiotherapy esophageal squamous cell carcinoma |
| url | https://www.tandfonline.com/doi/10.1080/07853890.2024.2445190 |
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