The Collection of Zoosporic Eufungi at the University of Michigan (CZEUM): introducing a new repository of barcoded Chytridiomyceta and Blastocladiomycota cultures
Abstract We formed the Collection of Zoosporic Eufungi at the University of Michigan (CZEUM) in 2018 as a cryopreserved fungal collection consolidating the University of Maine Culture Collection (UMCC, or JEL), the University of Alabama Chytrid Culture Collection (UACCC), and additional zoosporic eu...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-10-01
|
| Series: | IMA Fungus |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s43008-020-00041-z |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832569510506266624 |
|---|---|
| author | D. Rabern Simmons Anne E. Bonds Buck T. Castillo Rebecca A. Clemons Alex D. Glasco Jillian M. Myers Natasha Thapa Peter M. Letcher Martha J. Powell Joyce E. Longcore Timothy Y. James |
| author_facet | D. Rabern Simmons Anne E. Bonds Buck T. Castillo Rebecca A. Clemons Alex D. Glasco Jillian M. Myers Natasha Thapa Peter M. Letcher Martha J. Powell Joyce E. Longcore Timothy Y. James |
| author_sort | D. Rabern Simmons |
| collection | DOAJ |
| description | Abstract We formed the Collection of Zoosporic Eufungi at the University of Michigan (CZEUM) in 2018 as a cryopreserved fungal collection consolidating the University of Maine Culture Collection (UMCC, or JEL), the University of Alabama Chytrid Culture Collection (UACCC), and additional zoosporic eufungal accessions. The CZEUM is established as a community resource containing 1045 cryopreserved cultures of Chytridiomycota, Monoblepharidomycota, and Blastocladiomycota, with 52 cultures being ex-type strains. We molecularly characterized 431 cultures by amplifying the majority of the rDNA operon in a single reaction, yielding an average fragment length of 4739 bp. We sequenced multiplexed samples with an Oxford Nanopore Technology MinION device and software, and demonstrate the method is accurate by producing sequences identical to published Sanger sequences. With these data, we generated a phylogeny of 882 zoosporic eufungi strains to produce the most comprehensive phylogeny of these taxa to date. The CZEUM is thus largely characterized by molecular data, which can guide instructors and researchers on future studies of these organisms. Cultures from the CZEUM can be purchased through an online portal. |
| format | Article |
| id | doaj-art-2a58f524db114096965b6983bfd5b49a |
| institution | Kabale University |
| issn | 2210-6359 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | BMC |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj-art-2a58f524db114096965b6983bfd5b49a2025-02-02T20:52:47ZengBMCIMA Fungus2210-63592020-10-0111112210.1186/s43008-020-00041-zThe Collection of Zoosporic Eufungi at the University of Michigan (CZEUM): introducing a new repository of barcoded Chytridiomyceta and Blastocladiomycota culturesD. Rabern Simmons0Anne E. Bonds1Buck T. Castillo2Rebecca A. Clemons3Alex D. Glasco4Jillian M. Myers5Natasha Thapa6Peter M. Letcher7Martha J. Powell8Joyce E. Longcore9Timothy Y. James10Department of Ecology and Evolutionary Biology, University of MichiganDepartment of Ecology and Evolutionary Biology, University of MichiganDepartment of Ecology and Evolutionary Biology, University of MichiganDepartment of Ecology and Evolutionary Biology, University of MichiganDepartment of Ecology and Evolutionary Biology, University of MichiganDepartment of Ecology and Evolutionary Biology, University of MichiganDepartment of Ecology and Evolutionary Biology, University of MichiganDepartment of Biological Sciences, The University of AlabamaDepartment of Biological Sciences, The University of AlabamaSchool of Biology & Ecology, University of MaineDepartment of Ecology and Evolutionary Biology, University of MichiganAbstract We formed the Collection of Zoosporic Eufungi at the University of Michigan (CZEUM) in 2018 as a cryopreserved fungal collection consolidating the University of Maine Culture Collection (UMCC, or JEL), the University of Alabama Chytrid Culture Collection (UACCC), and additional zoosporic eufungal accessions. The CZEUM is established as a community resource containing 1045 cryopreserved cultures of Chytridiomycota, Monoblepharidomycota, and Blastocladiomycota, with 52 cultures being ex-type strains. We molecularly characterized 431 cultures by amplifying the majority of the rDNA operon in a single reaction, yielding an average fragment length of 4739 bp. We sequenced multiplexed samples with an Oxford Nanopore Technology MinION device and software, and demonstrate the method is accurate by producing sequences identical to published Sanger sequences. With these data, we generated a phylogeny of 882 zoosporic eufungi strains to produce the most comprehensive phylogeny of these taxa to date. The CZEUM is thus largely characterized by molecular data, which can guide instructors and researchers on future studies of these organisms. Cultures from the CZEUM can be purchased through an online portal.http://link.springer.com/article/10.1186/s43008-020-00041-zCryopreservationOxford NanoporePhylogeny |
| spellingShingle | D. Rabern Simmons Anne E. Bonds Buck T. Castillo Rebecca A. Clemons Alex D. Glasco Jillian M. Myers Natasha Thapa Peter M. Letcher Martha J. Powell Joyce E. Longcore Timothy Y. James The Collection of Zoosporic Eufungi at the University of Michigan (CZEUM): introducing a new repository of barcoded Chytridiomyceta and Blastocladiomycota cultures IMA Fungus Cryopreservation Oxford Nanopore Phylogeny |
| title | The Collection of Zoosporic Eufungi at the University of Michigan (CZEUM): introducing a new repository of barcoded Chytridiomyceta and Blastocladiomycota cultures |
| title_full | The Collection of Zoosporic Eufungi at the University of Michigan (CZEUM): introducing a new repository of barcoded Chytridiomyceta and Blastocladiomycota cultures |
| title_fullStr | The Collection of Zoosporic Eufungi at the University of Michigan (CZEUM): introducing a new repository of barcoded Chytridiomyceta and Blastocladiomycota cultures |
| title_full_unstemmed | The Collection of Zoosporic Eufungi at the University of Michigan (CZEUM): introducing a new repository of barcoded Chytridiomyceta and Blastocladiomycota cultures |
| title_short | The Collection of Zoosporic Eufungi at the University of Michigan (CZEUM): introducing a new repository of barcoded Chytridiomyceta and Blastocladiomycota cultures |
| title_sort | collection of zoosporic eufungi at the university of michigan czeum introducing a new repository of barcoded chytridiomyceta and blastocladiomycota cultures |
| topic | Cryopreservation Oxford Nanopore Phylogeny |
| url | http://link.springer.com/article/10.1186/s43008-020-00041-z |
| work_keys_str_mv | AT drabernsimmons thecollectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT anneebonds thecollectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT bucktcastillo thecollectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT rebeccaaclemons thecollectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT alexdglasco thecollectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT jillianmmyers thecollectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT natashathapa thecollectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT petermletcher thecollectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT marthajpowell thecollectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT joyceelongcore thecollectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT timothyyjames thecollectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT drabernsimmons collectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT anneebonds collectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT bucktcastillo collectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT rebeccaaclemons collectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT alexdglasco collectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT jillianmmyers collectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT natashathapa collectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT petermletcher collectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT marthajpowell collectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT joyceelongcore collectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures AT timothyyjames collectionofzoosporiceufungiattheuniversityofmichiganczeumintroducinganewrepositoryofbarcodedchytridiomycetaandblastocladiomycotacultures |