Factors Influencing Oxidative Imbalance in Pulmonary Fibrosis: An Immunohistochemical Study
Background. Idiopathic Pulmonary Fibrosis (IPF) is a fatal lung disease of unknown etiology characterized by interstitial fibrosis determining irreversible distortion of pulmonary architecture. Reactive oxygen species (ROS) and markers of oxidative stress play a pivotal role in human IPF pathology,...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Pulmonary Medicine |
| Online Access: | http://dx.doi.org/10.1155/2011/421409 |
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| author | Simona Inghilleri Patrizia Morbini Ilaria Campo Michele Zorzetto Tiberio Oggionni Ernesto Pozzi Maurizio Luisetti |
| author_facet | Simona Inghilleri Patrizia Morbini Ilaria Campo Michele Zorzetto Tiberio Oggionni Ernesto Pozzi Maurizio Luisetti |
| author_sort | Simona Inghilleri |
| collection | DOAJ |
| description | Background. Idiopathic Pulmonary Fibrosis (IPF) is a fatal lung disease of unknown etiology characterized by interstitial fibrosis determining irreversible distortion of pulmonary architecture. Reactive oxygen species (ROS) and markers of oxidative stress play a pivotal role in human IPF pathology, possibly through induction of epithelial-mesenchymal transition (EMT). Methods. We investigated by immunohistochemistry, in UIP and COP tissue samples, the expression of most relevant markers of the molecular interplay involving RAGE, oxidant/antioxidant balance regulation, tissue nitrosylation, and mediators of EMT. Results. In both UIP and COP, the degree of RAGE expression was similarly high, while SODs and i-NOS, diffusely present in COP endoalveolar plugs, were almost absent in UIP fibroblast foci. A lower degree of tissue nitrosilation was observed in UIP than in COP. Conclusions. Fibroblast lesions of UIP and of COP share a similar degree of activation of RAGE, while antioxidant enzyme expression markedly reduced in UIP. |
| format | Article |
| id | doaj-art-2a3dfed024394594b3c4dd3b3cf8927e |
| institution | Kabale University |
| issn | 2090-1836 2090-1844 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Pulmonary Medicine |
| spelling | doaj-art-2a3dfed024394594b3c4dd3b3cf8927e2025-02-03T06:13:24ZengWileyPulmonary Medicine2090-18362090-18442011-01-01201110.1155/2011/421409421409Factors Influencing Oxidative Imbalance in Pulmonary Fibrosis: An Immunohistochemical StudySimona Inghilleri0Patrizia Morbini1Ilaria Campo2Michele Zorzetto3Tiberio Oggionni4Ernesto Pozzi5Maurizio Luisetti6Respiratory Disease, IRCCS Policlinico San Matteo Foundation, University of Pavia, Pavia, ItalyDepartment of Pathology, IRCCS Policlinico San Matteo Foundation, University of Pavia, Pavia, ItalyRespiratory Disease, IRCCS Policlinico San Matteo Foundation, University of Pavia, Pavia, ItalyRespiratory Disease, IRCCS Policlinico San Matteo Foundation, University of Pavia, Pavia, ItalyRespiratory Disease, IRCCS Policlinico San Matteo Foundation, University of Pavia, Pavia, ItalyRespiratory Disease, IRCCS Policlinico San Matteo Foundation, University of Pavia, Pavia, ItalyRespiratory Disease, IRCCS Policlinico San Matteo Foundation, University of Pavia, Pavia, ItalyBackground. Idiopathic Pulmonary Fibrosis (IPF) is a fatal lung disease of unknown etiology characterized by interstitial fibrosis determining irreversible distortion of pulmonary architecture. Reactive oxygen species (ROS) and markers of oxidative stress play a pivotal role in human IPF pathology, possibly through induction of epithelial-mesenchymal transition (EMT). Methods. We investigated by immunohistochemistry, in UIP and COP tissue samples, the expression of most relevant markers of the molecular interplay involving RAGE, oxidant/antioxidant balance regulation, tissue nitrosylation, and mediators of EMT. Results. In both UIP and COP, the degree of RAGE expression was similarly high, while SODs and i-NOS, diffusely present in COP endoalveolar plugs, were almost absent in UIP fibroblast foci. A lower degree of tissue nitrosilation was observed in UIP than in COP. Conclusions. Fibroblast lesions of UIP and of COP share a similar degree of activation of RAGE, while antioxidant enzyme expression markedly reduced in UIP.http://dx.doi.org/10.1155/2011/421409 |
| spellingShingle | Simona Inghilleri Patrizia Morbini Ilaria Campo Michele Zorzetto Tiberio Oggionni Ernesto Pozzi Maurizio Luisetti Factors Influencing Oxidative Imbalance in Pulmonary Fibrosis: An Immunohistochemical Study Pulmonary Medicine |
| title | Factors Influencing Oxidative Imbalance in Pulmonary Fibrosis: An Immunohistochemical Study |
| title_full | Factors Influencing Oxidative Imbalance in Pulmonary Fibrosis: An Immunohistochemical Study |
| title_fullStr | Factors Influencing Oxidative Imbalance in Pulmonary Fibrosis: An Immunohistochemical Study |
| title_full_unstemmed | Factors Influencing Oxidative Imbalance in Pulmonary Fibrosis: An Immunohistochemical Study |
| title_short | Factors Influencing Oxidative Imbalance in Pulmonary Fibrosis: An Immunohistochemical Study |
| title_sort | factors influencing oxidative imbalance in pulmonary fibrosis an immunohistochemical study |
| url | http://dx.doi.org/10.1155/2011/421409 |
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