Chick Early Amniotic Fluid Alleviates Dextran-Sulfate-Sodium-Induced Colitis in Mice via T-Cell Receptor Pathway
Ulcerative colitis (UC) is a chronic immune disease that is difficult to cure. We recently found that chick early amniotic fluid (ceAF) has notable anti-inflammatory and antioxidative properties, through its active components. This study demonstrates the potential of ceAF as a protective agent again...
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MDPI AG
2025-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/14/1/51 |
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| author | Fan Chen Yining Zhao Yanfa Dai Ning Sun Xuezheng Gao Jiajun Yin Zhenhe Zhou Ke-jia Wu |
| author_facet | Fan Chen Yining Zhao Yanfa Dai Ning Sun Xuezheng Gao Jiajun Yin Zhenhe Zhou Ke-jia Wu |
| author_sort | Fan Chen |
| collection | DOAJ |
| description | Ulcerative colitis (UC) is a chronic immune disease that is difficult to cure. We recently found that chick early amniotic fluid (ceAF) has notable anti-inflammatory and antioxidative properties, through its active components. This study demonstrates the potential of ceAF as a protective agent against UC. UPLC-MS mass spectrometry identified key components of ceAF, including various fatty acids and nucleosides. In vitro, ceAF improved viability in DSS-induced Caco-2 cells, reduced pro-inflammatory cytokines IL-1β and TNF-α, and increased the anti-inflammatory cytokine IL-10. It also upregulated the tight junction proteins ZO-1 and occludin. In DSS-induced UC mice, ceAF treatment alleviated weight loss, colon shortening, and disease activity, while improving histopathology, crypt depth, and colonic fibrosis. Mechanistically, ceAF’s anti-inflammatory effects are mediated by inhibiting the overactivation of TCR signaling through the LCK/ZAP70/LAT pathway. Our findings suggest that ceAF could be a valuable nutritional intervention for UC, potentially enhancing existing functional foods aimed at managing this condition. |
| format | Article |
| id | doaj-art-2a1cffac670f47c0948c96523f0d3251 |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj-art-2a1cffac670f47c0948c96523f0d32512025-01-24T13:19:19ZengMDPI AGAntioxidants2076-39212025-01-011415110.3390/antiox14010051Chick Early Amniotic Fluid Alleviates Dextran-Sulfate-Sodium-Induced Colitis in Mice via T-Cell Receptor PathwayFan Chen0Yining Zhao1Yanfa Dai2Ning Sun3Xuezheng Gao4Jiajun Yin5Zhenhe Zhou6Ke-jia Wu7Department of Psychiatry, The Affiliated Wuxi Mental Health Center of Jiangnan University, Wuxi 214151, ChinaWuxi School of Medicine, Jiangnan University, Wuxi 214082, ChinaWuxi School of Medicine, Jiangnan University, Wuxi 214082, ChinaWuxi School of Medicine, Jiangnan University, Wuxi 214082, ChinaDepartment of Psychiatry, The Affiliated Wuxi Mental Health Center of Jiangnan University, Wuxi 214151, ChinaDepartment of Psychiatry, The Affiliated Wuxi Mental Health Center of Jiangnan University, Wuxi 214151, ChinaDepartment of Psychiatry, The Affiliated Wuxi Mental Health Center of Jiangnan University, Wuxi 214151, ChinaDepartment of Psychiatry, The Affiliated Wuxi Mental Health Center of Jiangnan University, Wuxi 214151, ChinaUlcerative colitis (UC) is a chronic immune disease that is difficult to cure. We recently found that chick early amniotic fluid (ceAF) has notable anti-inflammatory and antioxidative properties, through its active components. This study demonstrates the potential of ceAF as a protective agent against UC. UPLC-MS mass spectrometry identified key components of ceAF, including various fatty acids and nucleosides. In vitro, ceAF improved viability in DSS-induced Caco-2 cells, reduced pro-inflammatory cytokines IL-1β and TNF-α, and increased the anti-inflammatory cytokine IL-10. It also upregulated the tight junction proteins ZO-1 and occludin. In DSS-induced UC mice, ceAF treatment alleviated weight loss, colon shortening, and disease activity, while improving histopathology, crypt depth, and colonic fibrosis. Mechanistically, ceAF’s anti-inflammatory effects are mediated by inhibiting the overactivation of TCR signaling through the LCK/ZAP70/LAT pathway. Our findings suggest that ceAF could be a valuable nutritional intervention for UC, potentially enhancing existing functional foods aimed at managing this condition.https://www.mdpi.com/2076-3921/14/1/51ulcerative colitisinflammatory bowel diseasechick early amniotic fluidTCR signaling pathwaytyrosine protein kinase |
| spellingShingle | Fan Chen Yining Zhao Yanfa Dai Ning Sun Xuezheng Gao Jiajun Yin Zhenhe Zhou Ke-jia Wu Chick Early Amniotic Fluid Alleviates Dextran-Sulfate-Sodium-Induced Colitis in Mice via T-Cell Receptor Pathway Antioxidants ulcerative colitis inflammatory bowel disease chick early amniotic fluid TCR signaling pathway tyrosine protein kinase |
| title | Chick Early Amniotic Fluid Alleviates Dextran-Sulfate-Sodium-Induced Colitis in Mice via T-Cell Receptor Pathway |
| title_full | Chick Early Amniotic Fluid Alleviates Dextran-Sulfate-Sodium-Induced Colitis in Mice via T-Cell Receptor Pathway |
| title_fullStr | Chick Early Amniotic Fluid Alleviates Dextran-Sulfate-Sodium-Induced Colitis in Mice via T-Cell Receptor Pathway |
| title_full_unstemmed | Chick Early Amniotic Fluid Alleviates Dextran-Sulfate-Sodium-Induced Colitis in Mice via T-Cell Receptor Pathway |
| title_short | Chick Early Amniotic Fluid Alleviates Dextran-Sulfate-Sodium-Induced Colitis in Mice via T-Cell Receptor Pathway |
| title_sort | chick early amniotic fluid alleviates dextran sulfate sodium induced colitis in mice via t cell receptor pathway |
| topic | ulcerative colitis inflammatory bowel disease chick early amniotic fluid TCR signaling pathway tyrosine protein kinase |
| url | https://www.mdpi.com/2076-3921/14/1/51 |
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