Green Tea Extract Supplementation Induces the Lipolytic Pathway, Attenuates Obesity, and Reduces Low-Grade Inflammation in Mice Fed a High-Fat Diet
The aim of this study was to evaluate the effects of green tea Camellia sinensis extract on proinflammatory molecules and lipolytic protein levels in adipose tissue of diet-induced obese mice. Animals were randomized into four groups: CW (chow diet and water); CG (chow diet and water + green tea ext...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/635470 |
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| author | Cláudio A. Cunha Fábio S. Lira José C. Rosa Neto Gustavo D. Pimentel Gabriel I. H. Souza Camila Morais Gonçalves da Silva Cláudio T. de Souza Eliane B. Ribeiro Alexandra Christine Helena Frankland Sawaya Cláudia M. Oller do Nascimento Bruno Rodrigues Patrícia de Oliveira Carvalho Lila M. Oyama |
| author_facet | Cláudio A. Cunha Fábio S. Lira José C. Rosa Neto Gustavo D. Pimentel Gabriel I. H. Souza Camila Morais Gonçalves da Silva Cláudio T. de Souza Eliane B. Ribeiro Alexandra Christine Helena Frankland Sawaya Cláudia M. Oller do Nascimento Bruno Rodrigues Patrícia de Oliveira Carvalho Lila M. Oyama |
| author_sort | Cláudio A. Cunha |
| collection | DOAJ |
| description | The aim of this study was to evaluate the effects of green tea Camellia sinensis extract on proinflammatory molecules and lipolytic protein levels in adipose tissue of diet-induced obese mice. Animals were randomized into four groups: CW (chow diet and water); CG (chow diet and water + green tea extract); HW (high-fat diet and water); HG (high-fat diet and water + green tea extract). The mice were fed ad libitum with chow or high-fat diet and concomitantly supplemented (oral gavage) with 400 mg/kg body weight/day of green tea extract (CG and HG, resp.). The treatments were performed for eight weeks. UPLC showed that in 10 mg/mL green tea extract, there were 15 μg/mg epigallocatechin, 95 μg/mg epigallocatechin gallate, 20.8 μg/mg epicatechin gallate, and 4.9 μg/mg gallocatechin gallate. Green tea administered concomitantly with a high-fat diet increased HSL, ABHD5, and perilipin in mesenteric adipose tissue, and this was associated with reduced body weight and adipose tissue gain. Further, we observed that green tea supplementation reduced inflammatory cytokine TNFα levels, as well as TLR4, MYD88, and TRAF6 proinflammatory signalling. Our results show that green tea increases the lipolytic pathway and reduces adipose tissue, and this may explain the attenuation of low-grade inflammation in obese mice. |
| format | Article |
| id | doaj-art-292ad09f109048c2a9f85d85eaddb39c |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-292ad09f109048c2a9f85d85eaddb39c2025-02-03T06:12:56ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/635470635470Green Tea Extract Supplementation Induces the Lipolytic Pathway, Attenuates Obesity, and Reduces Low-Grade Inflammation in Mice Fed a High-Fat DietCláudio A. Cunha0Fábio S. Lira1José C. Rosa Neto2Gustavo D. Pimentel3Gabriel I. H. Souza4Camila Morais Gonçalves da Silva5Cláudio T. de Souza6Eliane B. Ribeiro7Alexandra Christine Helena Frankland Sawaya8Cláudia M. Oller do Nascimento9Bruno Rodrigues10Patrícia de Oliveira Carvalho11Lila M. Oyama12Departamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo (UNIFESP), 04023-060 São Paulo, SP, BrazilLaboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, University of Southern Santa Catarina, 88806-000 Criciúma, SC, BrazilDepartamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo (UNIFESP), 04023-060 São Paulo, SP, BrazilDepartment of Internal Medicine, State University of Campinas, 13083-887 Campinas, SP, BrazilDepartamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo (UNIFESP), 04023-060 São Paulo, SP, BrazilLaboratory of Multidisciplinary Research, São Francisco University (USF), 12916-900 Bragança Paulista, SP, BrazilLaboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, University of Southern Santa Catarina, 88806-000 Criciúma, SC, BrazilDepartamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo (UNIFESP), 04023-060 São Paulo, SP, BrazilDepartment of Plant Biology, Institute of Biology, State University of Campinas (UNICAMP), 13083-970 Campinas, SP, BrazilDepartamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo (UNIFESP), 04023-060 São Paulo, SP, BrazilHuman Moviment Laboratory, São Judas Tadeu University, 05503-001 São Paulo, SP, BrazilLaboratory of Multidisciplinary Research, São Francisco University (USF), 12916-900 Bragança Paulista, SP, BrazilDepartamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo (UNIFESP), 04023-060 São Paulo, SP, BrazilThe aim of this study was to evaluate the effects of green tea Camellia sinensis extract on proinflammatory molecules and lipolytic protein levels in adipose tissue of diet-induced obese mice. Animals were randomized into four groups: CW (chow diet and water); CG (chow diet and water + green tea extract); HW (high-fat diet and water); HG (high-fat diet and water + green tea extract). The mice were fed ad libitum with chow or high-fat diet and concomitantly supplemented (oral gavage) with 400 mg/kg body weight/day of green tea extract (CG and HG, resp.). The treatments were performed for eight weeks. UPLC showed that in 10 mg/mL green tea extract, there were 15 μg/mg epigallocatechin, 95 μg/mg epigallocatechin gallate, 20.8 μg/mg epicatechin gallate, and 4.9 μg/mg gallocatechin gallate. Green tea administered concomitantly with a high-fat diet increased HSL, ABHD5, and perilipin in mesenteric adipose tissue, and this was associated with reduced body weight and adipose tissue gain. Further, we observed that green tea supplementation reduced inflammatory cytokine TNFα levels, as well as TLR4, MYD88, and TRAF6 proinflammatory signalling. Our results show that green tea increases the lipolytic pathway and reduces adipose tissue, and this may explain the attenuation of low-grade inflammation in obese mice.http://dx.doi.org/10.1155/2013/635470 |
| spellingShingle | Cláudio A. Cunha Fábio S. Lira José C. Rosa Neto Gustavo D. Pimentel Gabriel I. H. Souza Camila Morais Gonçalves da Silva Cláudio T. de Souza Eliane B. Ribeiro Alexandra Christine Helena Frankland Sawaya Cláudia M. Oller do Nascimento Bruno Rodrigues Patrícia de Oliveira Carvalho Lila M. Oyama Green Tea Extract Supplementation Induces the Lipolytic Pathway, Attenuates Obesity, and Reduces Low-Grade Inflammation in Mice Fed a High-Fat Diet Mediators of Inflammation |
| title | Green Tea Extract Supplementation Induces the Lipolytic Pathway, Attenuates Obesity, and Reduces Low-Grade Inflammation in Mice Fed a High-Fat Diet |
| title_full | Green Tea Extract Supplementation Induces the Lipolytic Pathway, Attenuates Obesity, and Reduces Low-Grade Inflammation in Mice Fed a High-Fat Diet |
| title_fullStr | Green Tea Extract Supplementation Induces the Lipolytic Pathway, Attenuates Obesity, and Reduces Low-Grade Inflammation in Mice Fed a High-Fat Diet |
| title_full_unstemmed | Green Tea Extract Supplementation Induces the Lipolytic Pathway, Attenuates Obesity, and Reduces Low-Grade Inflammation in Mice Fed a High-Fat Diet |
| title_short | Green Tea Extract Supplementation Induces the Lipolytic Pathway, Attenuates Obesity, and Reduces Low-Grade Inflammation in Mice Fed a High-Fat Diet |
| title_sort | green tea extract supplementation induces the lipolytic pathway attenuates obesity and reduces low grade inflammation in mice fed a high fat diet |
| url | http://dx.doi.org/10.1155/2013/635470 |
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