Dichotomous Effect of Caffeine, Curcumin, and Naringenin on Genomic DNA of Normal and Diabetic Subjects
Nutraceutical compounds show antioxidant and prooxidant properties under stress conditions like cancer, diabetes, and other diseases. The objective of this study is to find the dichotomic behavior of caffeine, curcumin, and naringenin on DNA of diabetic and normal subjects in the presence and absenc...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2014/649261 |
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| author | Debarati Chattopadhyay Ashok Somaiah Divya Raghunathan Kavitha Thirumurugan |
| author_facet | Debarati Chattopadhyay Ashok Somaiah Divya Raghunathan Kavitha Thirumurugan |
| author_sort | Debarati Chattopadhyay |
| collection | DOAJ |
| description | Nutraceutical compounds show antioxidant and prooxidant properties under stress conditions like cancer, diabetes, and other diseases. The objective of this study is to find the dichotomic behavior of caffeine, curcumin, and naringenin on DNA of diabetic and normal subjects in the presence and absence of copper, hydrogen peroxide, and complex of copper-hydrogen peroxide. Hydrogen peroxide releases hydroxyl free radicals (•OH) on oxidation of Cu (I) to Cu (II) through Fenton-type reaction to cause DNA damage. In the results, agarose gel electrophoretic pattern speculates the prooxidant effect of caffeine and antioxidant effect of curcumin on DNA in the presence of copper and hydrogen peroxide. UV-Vis spectral analysis shows hyperchromism on addition of DNA to caffeine, hypochromism with curcumin, and subtle changes with naringenin. The chosen nutraceuticals act as inducers and quenchers of oxidative free radicals arising from diabetes. |
| format | Article |
| id | doaj-art-28de088b057149d390823d1de695fab6 |
| institution | Kabale University |
| issn | 2090-908X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Scientifica |
| spelling | doaj-art-28de088b057149d390823d1de695fab62025-02-03T01:31:23ZengWileyScientifica2090-908X2014-01-01201410.1155/2014/649261649261Dichotomous Effect of Caffeine, Curcumin, and Naringenin on Genomic DNA of Normal and Diabetic SubjectsDebarati Chattopadhyay0Ashok Somaiah1Divya Raghunathan2Kavitha Thirumurugan3Structural Biology Lab, Centre for Biomedical Research, School of Bio Sciences & Technology, VIT University, Vellore, Tamil Nadu 632014, IndiaStructural Biology Lab, Centre for Biomedical Research, School of Bio Sciences & Technology, VIT University, Vellore, Tamil Nadu 632014, IndiaStructural Biology Lab, Centre for Biomedical Research, School of Bio Sciences & Technology, VIT University, Vellore, Tamil Nadu 632014, IndiaStructural Biology Lab, Centre for Biomedical Research, School of Bio Sciences & Technology, VIT University, Vellore, Tamil Nadu 632014, IndiaNutraceutical compounds show antioxidant and prooxidant properties under stress conditions like cancer, diabetes, and other diseases. The objective of this study is to find the dichotomic behavior of caffeine, curcumin, and naringenin on DNA of diabetic and normal subjects in the presence and absence of copper, hydrogen peroxide, and complex of copper-hydrogen peroxide. Hydrogen peroxide releases hydroxyl free radicals (•OH) on oxidation of Cu (I) to Cu (II) through Fenton-type reaction to cause DNA damage. In the results, agarose gel electrophoretic pattern speculates the prooxidant effect of caffeine and antioxidant effect of curcumin on DNA in the presence of copper and hydrogen peroxide. UV-Vis spectral analysis shows hyperchromism on addition of DNA to caffeine, hypochromism with curcumin, and subtle changes with naringenin. The chosen nutraceuticals act as inducers and quenchers of oxidative free radicals arising from diabetes.http://dx.doi.org/10.1155/2014/649261 |
| spellingShingle | Debarati Chattopadhyay Ashok Somaiah Divya Raghunathan Kavitha Thirumurugan Dichotomous Effect of Caffeine, Curcumin, and Naringenin on Genomic DNA of Normal and Diabetic Subjects Scientifica |
| title | Dichotomous Effect of Caffeine, Curcumin, and Naringenin on Genomic DNA of Normal and Diabetic Subjects |
| title_full | Dichotomous Effect of Caffeine, Curcumin, and Naringenin on Genomic DNA of Normal and Diabetic Subjects |
| title_fullStr | Dichotomous Effect of Caffeine, Curcumin, and Naringenin on Genomic DNA of Normal and Diabetic Subjects |
| title_full_unstemmed | Dichotomous Effect of Caffeine, Curcumin, and Naringenin on Genomic DNA of Normal and Diabetic Subjects |
| title_short | Dichotomous Effect of Caffeine, Curcumin, and Naringenin on Genomic DNA of Normal and Diabetic Subjects |
| title_sort | dichotomous effect of caffeine curcumin and naringenin on genomic dna of normal and diabetic subjects |
| url | http://dx.doi.org/10.1155/2014/649261 |
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