Ameliorative effect of Lacticaseibacillus rhamnosus Fmb14 from Chinese yogurt on hyperuricemia
Hyperuricemia is a critical threat to human health, and a high inosine diet can increase the prevalence of it. Lacticaseibacillus rhamnosus Fmb14 was isolated from traditional fermented Chinese yogurt, and its inosine degradation rate reached 36.3 % at 109 CFU/mL for 24 h. LC-MS analysis revealed th...
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002506 |
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| author | Hongyuan Zhao Xiaoyu Chen Fanqiang Meng Libang Zhou Xinyi Pang Zhaoxin Lu Yingjian Lu |
| author_facet | Hongyuan Zhao Xiaoyu Chen Fanqiang Meng Libang Zhou Xinyi Pang Zhaoxin Lu Yingjian Lu |
| author_sort | Hongyuan Zhao |
| collection | DOAJ |
| description | Hyperuricemia is a critical threat to human health, and a high inosine diet can increase the prevalence of it. Lacticaseibacillus rhamnosus Fmb14 was isolated from traditional fermented Chinese yogurt, and its inosine degradation rate reached 36.3 % at 109 CFU/mL for 24 h. LC-MS analysis revealed that high concentrations of inosine could activate compensatory metabolic pathways of L. rhamnosus Fmb14 to catalyse inosine as an energy source and produce intracellular folic acid and riboflavin. The contents of folic acid and riboflavin were 6.0 and 4.3 fold increased after inosine treatment in the cell-free extracts (CFE). L. rhamnosus Fmb14 CFE treatment ameliorates hyperuricemia through xanthine oxidase (XOD) inhibition and ATP-binding cassette subfamily G member 2 (ABCG2) promotion, both of which are responsible for uric acid (UA) synthesis and secretion in HepG2 and Caco2 cells, respectively. The in vivo results showed that the serum UA level decreased from 236.28 to 149.28 μmol/L after 8 weeks of oral administration of L. rhamnosus Fmb14 in inosine-induced hyperuricemia model mice. Our results revealed that L. rhamnosus Fmb14 has a potential as a biological therapeutic agent in hyperuricemia prevention. |
| format | Article |
| id | doaj-art-28b107c39c2e49f5a7af4450a31071d5 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
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| series | Food Science and Human Wellness |
| spelling | doaj-art-28b107c39c2e49f5a7af4450a31071d52025-02-03T07:06:24ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112413791390Ameliorative effect of Lacticaseibacillus rhamnosus Fmb14 from Chinese yogurt on hyperuricemiaHongyuan Zhao0Xiaoyu Chen1Fanqiang Meng2Libang Zhou3Xinyi Pang4Zhaoxin Lu5Yingjian Lu6College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; Corresponding authors.College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China; Corresponding authors.Hyperuricemia is a critical threat to human health, and a high inosine diet can increase the prevalence of it. Lacticaseibacillus rhamnosus Fmb14 was isolated from traditional fermented Chinese yogurt, and its inosine degradation rate reached 36.3 % at 109 CFU/mL for 24 h. LC-MS analysis revealed that high concentrations of inosine could activate compensatory metabolic pathways of L. rhamnosus Fmb14 to catalyse inosine as an energy source and produce intracellular folic acid and riboflavin. The contents of folic acid and riboflavin were 6.0 and 4.3 fold increased after inosine treatment in the cell-free extracts (CFE). L. rhamnosus Fmb14 CFE treatment ameliorates hyperuricemia through xanthine oxidase (XOD) inhibition and ATP-binding cassette subfamily G member 2 (ABCG2) promotion, both of which are responsible for uric acid (UA) synthesis and secretion in HepG2 and Caco2 cells, respectively. The in vivo results showed that the serum UA level decreased from 236.28 to 149.28 μmol/L after 8 weeks of oral administration of L. rhamnosus Fmb14 in inosine-induced hyperuricemia model mice. Our results revealed that L. rhamnosus Fmb14 has a potential as a biological therapeutic agent in hyperuricemia prevention.http://www.sciencedirect.com/science/article/pii/S2213453022002506Lacticaseibacillus rhamnosus Fmb14,HyperuricemiaInosine degradationUric acid metabolism |
| spellingShingle | Hongyuan Zhao Xiaoyu Chen Fanqiang Meng Libang Zhou Xinyi Pang Zhaoxin Lu Yingjian Lu Ameliorative effect of Lacticaseibacillus rhamnosus Fmb14 from Chinese yogurt on hyperuricemia Food Science and Human Wellness Lacticaseibacillus rhamnosus Fmb14, Hyperuricemia Inosine degradation Uric acid metabolism |
| title | Ameliorative effect of Lacticaseibacillus rhamnosus Fmb14 from Chinese yogurt on hyperuricemia |
| title_full | Ameliorative effect of Lacticaseibacillus rhamnosus Fmb14 from Chinese yogurt on hyperuricemia |
| title_fullStr | Ameliorative effect of Lacticaseibacillus rhamnosus Fmb14 from Chinese yogurt on hyperuricemia |
| title_full_unstemmed | Ameliorative effect of Lacticaseibacillus rhamnosus Fmb14 from Chinese yogurt on hyperuricemia |
| title_short | Ameliorative effect of Lacticaseibacillus rhamnosus Fmb14 from Chinese yogurt on hyperuricemia |
| title_sort | ameliorative effect of lacticaseibacillus rhamnosus fmb14 from chinese yogurt on hyperuricemia |
| topic | Lacticaseibacillus rhamnosus Fmb14, Hyperuricemia Inosine degradation Uric acid metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002506 |
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