Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium
Live attenuated bacterial vectors, such as Salmonella typhimurium, have shown promise as delivery vehicles for DNA. We have examined two new strains of S. typhimurium and their impact on dendritic cell maturation (CD12-sifA/aroC mutant and WT05-ssaV/aroC, both in TML background). Strain WT05 matured...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2010.37 |
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| _version_ | 1832563134747901952 |
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| author | Agnieszka Michael Justin John Brendan Meyer Hardev Pandha |
| author_facet | Agnieszka Michael Justin John Brendan Meyer Hardev Pandha |
| author_sort | Agnieszka Michael |
| collection | DOAJ |
| description | Live attenuated bacterial vectors, such as Salmonella typhimurium, have shown promise as delivery vehicles for DNA. We have examined two new strains of S. typhimurium and their impact on dendritic cell maturation (CD12-sifA/aroC mutant and WT05-ssaV/aroC, both in TML background). Strain WT05 matured dendritic cells in a more efficient way; caused higher release of cytokines TNF-α, IL-12, IL-1β; and was efficient for gene transfer. These findings suggest that the genetic background of the attenuation can influence the pattern of inflammatory immune response to Salmonella infection. |
| format | Article |
| id | doaj-art-27c5ace6b4914e9ba6c429bf2c97940b |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-27c5ace6b4914e9ba6c429bf2c97940b2025-02-03T01:20:56ZengWileyThe Scientific World Journal1537-744X2010-01-011039340110.1100/tsw.2010.37Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimuriumAgnieszka Michael0Justin John1Brendan Meyer2Hardev Pandha3Postgraduate Medical School – Oncology, University of Surrey, Manor Park, Guildford, UKOncology Department, St George's University of London, United KingdomOncology Department, St George's University of London, United KingdomPostgraduate Medical School – Oncology, University of Surrey, Manor Park, Guildford, UKLive attenuated bacterial vectors, such as Salmonella typhimurium, have shown promise as delivery vehicles for DNA. We have examined two new strains of S. typhimurium and their impact on dendritic cell maturation (CD12-sifA/aroC mutant and WT05-ssaV/aroC, both in TML background). Strain WT05 matured dendritic cells in a more efficient way; caused higher release of cytokines TNF-α, IL-12, IL-1β; and was efficient for gene transfer. These findings suggest that the genetic background of the attenuation can influence the pattern of inflammatory immune response to Salmonella infection.http://dx.doi.org/10.1100/tsw.2010.37 |
| spellingShingle | Agnieszka Michael Justin John Brendan Meyer Hardev Pandha Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium The Scientific World Journal |
| title | Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium |
| title_full | Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium |
| title_fullStr | Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium |
| title_full_unstemmed | Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium |
| title_short | Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium |
| title_sort | activation and genetic modification of human monocyte derived dendritic cells using attenuated salmonella typhimurium |
| url | http://dx.doi.org/10.1100/tsw.2010.37 |
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