Enhanced Molybdenum Recovery Achieved by a Complex of Porous Material-Immobilized Surface-Engineered Yeast in Development of a Sustainable Biosorption Technology

Molybdenum (Mo) is a critical industrial metal valued for its corrosion resistance and strength-enhancing properties. However, increasing demand necessitates more efficient and sustainable recovery methods. Bio-recovery of Mo by biosorption is a promising resolution, especially by the use of surface...

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Main Authors: Thiti Jittayasotorn, Kentaro Kojima, Audrey Stephanie, Kaho Nakamura, Hernando P. Bacosa, Kengo Kubota, Masanobu Kamitakahara, Chihiro Inoue, Mei-Fang Chien
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/5/1034
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author Thiti Jittayasotorn
Kentaro Kojima
Audrey Stephanie
Kaho Nakamura
Hernando P. Bacosa
Kengo Kubota
Masanobu Kamitakahara
Chihiro Inoue
Mei-Fang Chien
author_facet Thiti Jittayasotorn
Kentaro Kojima
Audrey Stephanie
Kaho Nakamura
Hernando P. Bacosa
Kengo Kubota
Masanobu Kamitakahara
Chihiro Inoue
Mei-Fang Chien
author_sort Thiti Jittayasotorn
collection DOAJ
description Molybdenum (Mo) is a critical industrial metal valued for its corrosion resistance and strength-enhancing properties. However, increasing demand necessitates more efficient and sustainable recovery methods. Bio-recovery of Mo by biosorption is a promising resolution, especially by the use of surface-engineered microbes that express metal binding proteins on its cell surface. This study investigates the potential of <i>Saccharomyces cerevisiae</i> strain ScBp6, which displays a molybdate-binding protein (ModE) on its cell surface, immobilized on porous materials. Our findings reveal that polyurethane sponges (PS) significantly outperform ceramic materials in yeast immobilization, entrapping 1.76 × 10<sup>7</sup> cells per sponge compared to 1.70 × 10<sup>6</sup> cells per ceramic cube. Furthermore, the yeast–PS complex demonstrated superior Mo adsorption, reaching 2.16 pg Mo per yeast cell under 10 ppm Mo conditions, comparable to free yeast cells (1.96 pg Mo per yeast cell). These results establish PS as an effective and scalable platform for Mo recovery, offering high biosorption efficiency, reusability, and potential for industrial wastewater treatment applications.
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series Microorganisms
spelling doaj-art-26ed746f6f1540a6b87fd6b2e034b3212025-08-20T01:56:35ZengMDPI AGMicroorganisms2076-26072025-04-01135103410.3390/microorganisms13051034Enhanced Molybdenum Recovery Achieved by a Complex of Porous Material-Immobilized Surface-Engineered Yeast in Development of a Sustainable Biosorption TechnologyThiti Jittayasotorn0Kentaro Kojima1Audrey Stephanie2Kaho Nakamura3Hernando P. Bacosa4Kengo Kubota5Masanobu Kamitakahara6Chihiro Inoue7Mei-Fang Chien8Department of Environmental Studies for Advanced Society, Graduate School of Environmental Studies, Tohoku University, 6-6-20 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, JapanDepartment of Environmental Studies for Advanced Society, Graduate School of Environmental Studies, Tohoku University, 6-6-20 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, JapanDepartment of Environmental Studies for Advanced Society, Graduate School of Environmental Studies, Tohoku University, 6-6-20 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, JapanDepartment of Environmental Studies for Advanced Society, Graduate School of Environmental Studies, Tohoku University, 6-6-20 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, JapanDepartment of Environmental Science, School of Interdisciplinary Studies, Mindanao State University-Iligan Institute of Technology, Andres Bonifacio Avenue, Iligan 9200, PhilippinesDepartment of Frontier Sciences for Advanced Environment, Graduate of Environmental Studies, and Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, JapanDepartment of Environmental Studies for Advanced Society, Graduate School of Environmental Studies, Tohoku University, 6-6-20 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, JapanDepartment of Environmental Studies for Advanced Society, Graduate School of Environmental Studies, Tohoku University, 6-6-20 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, JapanDepartment of Environmental Studies for Advanced Society, Graduate School of Environmental Studies, Tohoku University, 6-6-20 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, JapanMolybdenum (Mo) is a critical industrial metal valued for its corrosion resistance and strength-enhancing properties. However, increasing demand necessitates more efficient and sustainable recovery methods. Bio-recovery of Mo by biosorption is a promising resolution, especially by the use of surface-engineered microbes that express metal binding proteins on its cell surface. This study investigates the potential of <i>Saccharomyces cerevisiae</i> strain ScBp6, which displays a molybdate-binding protein (ModE) on its cell surface, immobilized on porous materials. Our findings reveal that polyurethane sponges (PS) significantly outperform ceramic materials in yeast immobilization, entrapping 1.76 × 10<sup>7</sup> cells per sponge compared to 1.70 × 10<sup>6</sup> cells per ceramic cube. Furthermore, the yeast–PS complex demonstrated superior Mo adsorption, reaching 2.16 pg Mo per yeast cell under 10 ppm Mo conditions, comparable to free yeast cells (1.96 pg Mo per yeast cell). These results establish PS as an effective and scalable platform for Mo recovery, offering high biosorption efficiency, reusability, and potential for industrial wastewater treatment applications.https://www.mdpi.com/2076-2607/13/5/1034bio-recoverymolybdaterare metal recoveryimmobilizationcell-surface display<i>Saccharomyces cerevisiae</i>
spellingShingle Thiti Jittayasotorn
Kentaro Kojima
Audrey Stephanie
Kaho Nakamura
Hernando P. Bacosa
Kengo Kubota
Masanobu Kamitakahara
Chihiro Inoue
Mei-Fang Chien
Enhanced Molybdenum Recovery Achieved by a Complex of Porous Material-Immobilized Surface-Engineered Yeast in Development of a Sustainable Biosorption Technology
Microorganisms
bio-recovery
molybdate
rare metal recovery
immobilization
cell-surface display
<i>Saccharomyces cerevisiae</i>
title Enhanced Molybdenum Recovery Achieved by a Complex of Porous Material-Immobilized Surface-Engineered Yeast in Development of a Sustainable Biosorption Technology
title_full Enhanced Molybdenum Recovery Achieved by a Complex of Porous Material-Immobilized Surface-Engineered Yeast in Development of a Sustainable Biosorption Technology
title_fullStr Enhanced Molybdenum Recovery Achieved by a Complex of Porous Material-Immobilized Surface-Engineered Yeast in Development of a Sustainable Biosorption Technology
title_full_unstemmed Enhanced Molybdenum Recovery Achieved by a Complex of Porous Material-Immobilized Surface-Engineered Yeast in Development of a Sustainable Biosorption Technology
title_short Enhanced Molybdenum Recovery Achieved by a Complex of Porous Material-Immobilized Surface-Engineered Yeast in Development of a Sustainable Biosorption Technology
title_sort enhanced molybdenum recovery achieved by a complex of porous material immobilized surface engineered yeast in development of a sustainable biosorption technology
topic bio-recovery
molybdate
rare metal recovery
immobilization
cell-surface display
<i>Saccharomyces cerevisiae</i>
url https://www.mdpi.com/2076-2607/13/5/1034
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