Downregulation of Bcl-2 Expression by miR-34a Mediates Palmitate-Induced Min6 Cells Apoptosis
Recent studies have demonstrated that the expression of miR-34a is significantly upregulated and associated with cell apoptosis in pancreatic β-cell treated with palmitate. Nevertheless, the underlying detailed mechanism is largely unknown. Here, we showed that miR-34a was significantly induced in M...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2014/258695 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832553667090186240 |
|---|---|
| author | Xiaojie Lin Hongyu Guan Zhimin Huang Juan Liu Hai Li Guohong Wei Xiaopei Cao Yanbing Li |
| author_facet | Xiaojie Lin Hongyu Guan Zhimin Huang Juan Liu Hai Li Guohong Wei Xiaopei Cao Yanbing Li |
| author_sort | Xiaojie Lin |
| collection | DOAJ |
| description | Recent studies have demonstrated that the expression of miR-34a is significantly upregulated and associated with cell apoptosis in pancreatic β-cell treated with palmitate. Nevertheless, the underlying detailed mechanism is largely unknown. Here, we showed that miR-34a was significantly induced in Min6 pancreatic β-cell upon palmitate treatment. Elevated miR-34a promoted Min6 cell apoptosis. Intriguingly, ectopic expression of miR-34a lowered the expression of Bcl-2, an antiapoptotic protein. Luciferase reporter assay indicated the direct interaction of miR-34a with the Bcl-2 3′-UTR. Moreover, downregulated expression of Bcl-2 induced by palmitate could be restored by inhibition of miR-34a. We conclude that direct suppression of Bcl-2 by miR-34a accounts for palmitate-induced increased apoptosis rate in pancreatic β-cell. |
| format | Article |
| id | doaj-art-26dad0961eda42d68ac1c7faee22d3d9 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-26dad0961eda42d68ac1c7faee22d3d92025-02-03T05:53:31ZengWileyJournal of Diabetes Research2314-67452314-67532014-01-01201410.1155/2014/258695258695Downregulation of Bcl-2 Expression by miR-34a Mediates Palmitate-Induced Min6 Cells ApoptosisXiaojie Lin0Hongyu Guan1Zhimin Huang2Juan Liu3Hai Li4Guohong Wei5Xiaopei Cao6Yanbing Li7Department of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, Guangdong 510080, ChinaDepartment of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, Guangdong 510080, ChinaDepartment of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, Guangdong 510080, ChinaDepartment of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, Guangdong 510080, ChinaDepartment of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, Guangdong 510080, ChinaDepartment of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, Guangdong 510080, ChinaDepartment of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, Guangdong 510080, ChinaDepartment of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, Guangdong 510080, ChinaRecent studies have demonstrated that the expression of miR-34a is significantly upregulated and associated with cell apoptosis in pancreatic β-cell treated with palmitate. Nevertheless, the underlying detailed mechanism is largely unknown. Here, we showed that miR-34a was significantly induced in Min6 pancreatic β-cell upon palmitate treatment. Elevated miR-34a promoted Min6 cell apoptosis. Intriguingly, ectopic expression of miR-34a lowered the expression of Bcl-2, an antiapoptotic protein. Luciferase reporter assay indicated the direct interaction of miR-34a with the Bcl-2 3′-UTR. Moreover, downregulated expression of Bcl-2 induced by palmitate could be restored by inhibition of miR-34a. We conclude that direct suppression of Bcl-2 by miR-34a accounts for palmitate-induced increased apoptosis rate in pancreatic β-cell.http://dx.doi.org/10.1155/2014/258695 |
| spellingShingle | Xiaojie Lin Hongyu Guan Zhimin Huang Juan Liu Hai Li Guohong Wei Xiaopei Cao Yanbing Li Downregulation of Bcl-2 Expression by miR-34a Mediates Palmitate-Induced Min6 Cells Apoptosis Journal of Diabetes Research |
| title | Downregulation of Bcl-2 Expression by miR-34a Mediates Palmitate-Induced Min6 Cells Apoptosis |
| title_full | Downregulation of Bcl-2 Expression by miR-34a Mediates Palmitate-Induced Min6 Cells Apoptosis |
| title_fullStr | Downregulation of Bcl-2 Expression by miR-34a Mediates Palmitate-Induced Min6 Cells Apoptosis |
| title_full_unstemmed | Downregulation of Bcl-2 Expression by miR-34a Mediates Palmitate-Induced Min6 Cells Apoptosis |
| title_short | Downregulation of Bcl-2 Expression by miR-34a Mediates Palmitate-Induced Min6 Cells Apoptosis |
| title_sort | downregulation of bcl 2 expression by mir 34a mediates palmitate induced min6 cells apoptosis |
| url | http://dx.doi.org/10.1155/2014/258695 |
| work_keys_str_mv | AT xiaojielin downregulationofbcl2expressionbymir34amediatespalmitateinducedmin6cellsapoptosis AT hongyuguan downregulationofbcl2expressionbymir34amediatespalmitateinducedmin6cellsapoptosis AT zhiminhuang downregulationofbcl2expressionbymir34amediatespalmitateinducedmin6cellsapoptosis AT juanliu downregulationofbcl2expressionbymir34amediatespalmitateinducedmin6cellsapoptosis AT haili downregulationofbcl2expressionbymir34amediatespalmitateinducedmin6cellsapoptosis AT guohongwei downregulationofbcl2expressionbymir34amediatespalmitateinducedmin6cellsapoptosis AT xiaopeicao downregulationofbcl2expressionbymir34amediatespalmitateinducedmin6cellsapoptosis AT yanbingli downregulationofbcl2expressionbymir34amediatespalmitateinducedmin6cellsapoptosis |