Protocol for the simultaneous activation and lentiviral transduction of primary human T cells with artificial T cell receptors

Protocol for the simultaneous activation and lentiviral transduction of primary human T cells with artificial T cell receptors

Summary: T cell-based therapies hold immense promise, but their production remains time-consuming and technically complex. Here, we present a protocol that streamlines the activation and lentiviral transduction of primary human T cells with artificial receptors. We describe steps for T cell isolatio...

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Bibliographic Details
Main Authors: Jordan Yong Ming Tan, Jia Chi Tan, Chuqi Wang, Ling Wu, Nicholas R.J. Gascoigne, Shruti Bhatt
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
Cell culture
Cancer
Immunology
Molecular biology
Gene expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725000917
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http://www.sciencedirect.com/science/article/pii/S2666166725000917

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