Effects of anti-inflammatory drugs on fever and neutrophilia induced by Clostridium difficile toxin B
This study investigated the ability of Clostridium difficile toxin B, isolated from the VPI 10463 strain, to induce fever and neutrophilia in rats. Intravenous injection of toxin B (0.005–0.5 μg/kg) evoked a dose-dependent increase in body temperature. The febrile response to 0.5 μg/kg of the toxin...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1996-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935196000245 |
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| _version_ | 1832558160011853824 |
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| author | R. A. Cardoso A. A. Melo Filho M. C. C. Melo D. M. Lyerly T. D. Wilkins A. A. M. Lima R. A. Ribeiro G. E. P. Souza |
| author_facet | R. A. Cardoso A. A. Melo Filho M. C. C. Melo D. M. Lyerly T. D. Wilkins A. A. M. Lima R. A. Ribeiro G. E. P. Souza |
| author_sort | R. A. Cardoso |
| collection | DOAJ |
| description | This study investigated the ability of Clostridium difficile toxin B, isolated from the VPI 10463 strain, to induce fever and neutrophilia in rats. Intravenous injection of toxin B (0.005–0.5 μg/kg) evoked a dose-dependent increase in body temperature. The febrile response to 0.5 μg/kg of the toxin started in 2.5 h, peaked at 5 h, and subsided fully within 24 h. Toxin B also induced a dosedependent neutrophilia. Pretreatment with indomethacin (2 mg/kg, i.p.) did not affect the neutrophilia induced by toxin B, but significantly reduced the febrile response measured 4 to 8 h after toxin B injection. Dexamethasone (0.5 mg/ kg) also markedly diminished the febrile response induced by toxin B. These results show that Clostridium difficile toxin B induced a febrile response susceptible to inhibition by dexamethasone and indomethacin. Furthermore, they suggest that prostaglandins are not involved in the neutrophilia caused by this toxin. |
| format | Article |
| id | doaj-art-25e18328760a4675a6b67a97a49816fc |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1996-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-25e18328760a4675a6b67a97a49816fc2025-02-03T01:33:02ZengWileyMediators of Inflammation0962-93511466-18611996-01-015318318710.1155/S0962935196000245Effects of anti-inflammatory drugs on fever and neutrophilia induced by Clostridium difficile toxin BR. A. Cardoso0A. A. Melo Filho1M. C. C. Melo2D. M. Lyerly3T. D. Wilkins4A. A. M. Lima5R. A. Ribeiro6G. E. P. Souza7Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, USP Campus-14040-903, Ribeirão Preto, S.P., BrazilDepartment of Physiology and Pharmacology and Division of Infectious Diseases, Clinical Research Unit, Federal University of Ceará Fortaleza, C.E., BrazilLaboratory of Pharmacology, Faculty of Pharmaceutical Sciences, USP Campus-14040-903, Ribeirão Preto, S.P., BrazilDepartment of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USADepartment of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USADepartment of Physiology and Pharmacology and Division of Infectious Diseases, Clinical Research Unit, Federal University of Ceará Fortaleza, C.E., BrazilDepartment of Physiology and Pharmacology and Division of Infectious Diseases, Clinical Research Unit, Federal University of Ceará Fortaleza, C.E., BrazilLaboratory of Pharmacology, Faculty of Pharmaceutical Sciences, USP Campus-14040-903, Ribeirão Preto, S.P., BrazilThis study investigated the ability of Clostridium difficile toxin B, isolated from the VPI 10463 strain, to induce fever and neutrophilia in rats. Intravenous injection of toxin B (0.005–0.5 μg/kg) evoked a dose-dependent increase in body temperature. The febrile response to 0.5 μg/kg of the toxin started in 2.5 h, peaked at 5 h, and subsided fully within 24 h. Toxin B also induced a dosedependent neutrophilia. Pretreatment with indomethacin (2 mg/kg, i.p.) did not affect the neutrophilia induced by toxin B, but significantly reduced the febrile response measured 4 to 8 h after toxin B injection. Dexamethasone (0.5 mg/ kg) also markedly diminished the febrile response induced by toxin B. These results show that Clostridium difficile toxin B induced a febrile response susceptible to inhibition by dexamethasone and indomethacin. Furthermore, they suggest that prostaglandins are not involved in the neutrophilia caused by this toxin.http://dx.doi.org/10.1155/S0962935196000245 |
| spellingShingle | R. A. Cardoso A. A. Melo Filho M. C. C. Melo D. M. Lyerly T. D. Wilkins A. A. M. Lima R. A. Ribeiro G. E. P. Souza Effects of anti-inflammatory drugs on fever and neutrophilia induced by Clostridium difficile toxin B Mediators of Inflammation |
| title | Effects of anti-inflammatory drugs on fever and neutrophilia induced by Clostridium difficile toxin B |
| title_full | Effects of anti-inflammatory drugs on fever and neutrophilia induced by Clostridium difficile toxin B |
| title_fullStr | Effects of anti-inflammatory drugs on fever and neutrophilia induced by Clostridium difficile toxin B |
| title_full_unstemmed | Effects of anti-inflammatory drugs on fever and neutrophilia induced by Clostridium difficile toxin B |
| title_short | Effects of anti-inflammatory drugs on fever and neutrophilia induced by Clostridium difficile toxin B |
| title_sort | effects of anti inflammatory drugs on fever and neutrophilia induced by clostridium difficile toxin b |
| url | http://dx.doi.org/10.1155/S0962935196000245 |
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