Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO)
Although many studies have shown that administration of stem cells after focal cerebral ischemia improves brain damage, very little data are available concerning the damage induced by global cerebral ischemia. The latter causes neuronal death in selectively vulnerable areas, including the hippocampa...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2010/534925 |
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| _version_ | 1832558506213900288 |
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| author | Luisa Perasso Carla Emilia Cogo Debora Giunti Carlo Gandolfo Piero Ruggeri Antonio Uccelli Maurizio Balestrino |
| author_facet | Luisa Perasso Carla Emilia Cogo Debora Giunti Carlo Gandolfo Piero Ruggeri Antonio Uccelli Maurizio Balestrino |
| author_sort | Luisa Perasso |
| collection | DOAJ |
| description | Although many studies have shown that administration of stem cells after focal cerebral ischemia improves brain damage, very little data are available concerning the damage induced by global cerebral ischemia. The latter causes neuronal death in selectively vulnerable areas, including the hippocampal CA1 region. We tested the hypothesis that intravenous infusion of bone marrowderived stromal cells (mesenchimal stem cells, MSC) reduce brain damage after transient global ischemia. In adult male Sprague-Dawley rats transient global ischemia was induced using bilateral common carotid artery occlusion for 20 min in addition to controlled hypotension. Five days after, the animals were anaesthetized with urethane and the brain was fixed, sectioned and stained with hematoxylin-eosin to investigate histological damage. MSC did not fully protect against ischemic damage, as the number of viable neurons in this group was lower than in normal (sham-operated) rats. However, in MSC-treated rats the number of viable CA1 pyramidal neurons was significally higher than in rats that had been subjected to ischemia but not treated with MSC. We conclude that intravenous administration of MSC after transient global ischemia reduces hippocampal damage. |
| format | Article |
| id | doaj-art-252b510184d34ab7b95e4ada14019c0f |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-252b510184d34ab7b95e4ada14019c0f2025-02-03T01:32:12ZengWileyNeural Plasticity2090-59041687-54432010-01-01201010.1155/2010/534925534925Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO)Luisa Perasso0Carla Emilia Cogo1Debora Giunti2Carlo Gandolfo3Piero Ruggeri4Antonio Uccelli5Maurizio Balestrino6Department of Neuroscience, Ophthalmology and Genetics, University of Genova, Via De Toni 5, 16132 Genova, ItalyDepartment of Experimental Medicine, University of Genova, Viale Benedetto XV 3, 16132 Genova, ItalyDepartment of Neuroscience, Ophthalmology and Genetics, University of Genova, Via De Toni 5, 16132 Genova, ItalyDepartment of Neuroscience, Ophthalmology and Genetics, University of Genova, Via De Toni 5, 16132 Genova, ItalyDepartment of Experimental Medicine, University of Genova, Viale Benedetto XV 3, 16132 Genova, ItalyDepartment of Neuroscience, Ophthalmology and Genetics, University of Genova, Via De Toni 5, 16132 Genova, ItalyDepartment of Neuroscience, Ophthalmology and Genetics, University of Genova, Via De Toni 5, 16132 Genova, ItalyAlthough many studies have shown that administration of stem cells after focal cerebral ischemia improves brain damage, very little data are available concerning the damage induced by global cerebral ischemia. The latter causes neuronal death in selectively vulnerable areas, including the hippocampal CA1 region. We tested the hypothesis that intravenous infusion of bone marrowderived stromal cells (mesenchimal stem cells, MSC) reduce brain damage after transient global ischemia. In adult male Sprague-Dawley rats transient global ischemia was induced using bilateral common carotid artery occlusion for 20 min in addition to controlled hypotension. Five days after, the animals were anaesthetized with urethane and the brain was fixed, sectioned and stained with hematoxylin-eosin to investigate histological damage. MSC did not fully protect against ischemic damage, as the number of viable neurons in this group was lower than in normal (sham-operated) rats. However, in MSC-treated rats the number of viable CA1 pyramidal neurons was significally higher than in rats that had been subjected to ischemia but not treated with MSC. We conclude that intravenous administration of MSC after transient global ischemia reduces hippocampal damage.http://dx.doi.org/10.1155/2010/534925 |
| spellingShingle | Luisa Perasso Carla Emilia Cogo Debora Giunti Carlo Gandolfo Piero Ruggeri Antonio Uccelli Maurizio Balestrino Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO) Neural Plasticity |
| title | Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO) |
| title_full | Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO) |
| title_fullStr | Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO) |
| title_full_unstemmed | Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO) |
| title_short | Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO) |
| title_sort | systemic administration of mesenchymal stem cells increases neuron survival after global cerebral ischemia in vivo 2vo |
| url | http://dx.doi.org/10.1155/2010/534925 |
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