Peptide nucleic acid (PNA) clamps reduce amplification of host chloroplast and mitochondria rRNA gene sequences and increase detected diversity in 16S rRNA gene profiling analysis of oak-associated microbiota

Peptide nucleic acid (PNA) clamps reduce amplification of host chloroplast and mitochondria rRNA gene sequences and increase detected diversity in 16S rRNA gene profiling analysis of oak-associated microbiota

Abstract Background Acquiring representative bacterial 16S rRNA gene community profiles in plant microbiome studies can be challenging due to the excessive co-amplification of host chloroplast and mitochondrial rRNA gene sequences that reduce counts of plant-associated bacterial sequences. Peptide N...

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Bibliographic Details
Main Authors: Usman Hussain, Jim Downie, Amy Ellison, Sandra Denman, James McDonald, Marine C. Cambon
Format: Article
Language:English
Published: BMC 2025-01-01
Series:Environmental Microbiome
Subjects:
Chloroplast
Mitochondria
Microbiome
16S rRNA gene sequencing
PCR
qPCR
Online Access:https://doi.org/10.1186/s40793-025-00674-w
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https://doi.org/10.1186/s40793-025-00674-w

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