The Compound Expression of HSP90 and INOS in the Testis of Diabetic Rats as Cellular and Pathologic Adverse Effects of Diabetes
Introduction. Diabetes is increasingly prevalent at global level and associated with various impacts including the male reproductive system. Aims. This research is aimed at investigating the influence of diabetes on the localization and expression of HSP90 and iNOS in the testicular tissue of diabet...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2020/3906583 |
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| author | Ali Alsarhan Kawther Faisal Amawi Inas Saleh Al-Mazari Hashem Abu Hurirah Ahed J. Alkhatib |
| author_facet | Ali Alsarhan Kawther Faisal Amawi Inas Saleh Al-Mazari Hashem Abu Hurirah Ahed J. Alkhatib |
| author_sort | Ali Alsarhan |
| collection | DOAJ |
| description | Introduction. Diabetes is increasingly prevalent at global level and associated with various impacts including the male reproductive system. Aims. This research is aimed at investigating the influence of diabetes on the localization and expression of HSP90 and iNOS in the testicular tissue of diabetic rats. Methods. A diabetic model was developed through a single injection of alloxan monohydrate intraperitoneally (purchased from Sigma-Aldrich) 120 mg/kg body weight following fasting for 12 hrs. The experiment involved two groups, the control and diabetic groups with 10 albino rats in each group. Diabetes was considered if glucose concentration was ≥200 mg/dl. The experiment duration was for one month. After the experiment had finished, all rats were terminated and prepared for routine histological and immunohistochemical examination. Results. The results revealed that diabetes caused morphological changes at histological level in testicular tissue. Immunohistochemical examination showed that diabetes significantly upregulated the expression of both HSP90 and iNOS in the testicular tissue of diabetic rats as compared with that of the control group (p<0.001). Conclusion. Diabetes may induce adverse health effects on the male reproduction through upregulation of HSP90 and iNOS in the testicular tissue of diabetic rats. |
| format | Article |
| id | doaj-art-24d5128c271647949bb75347c99c9ba9 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-24d5128c271647949bb75347c99c9ba92025-02-03T05:53:52ZengWileyAnalytical Cellular Pathology2210-71772210-71852020-01-01202010.1155/2020/39065833906583The Compound Expression of HSP90 and INOS in the Testis of Diabetic Rats as Cellular and Pathologic Adverse Effects of DiabetesAli Alsarhan0Kawther Faisal Amawi1Inas Saleh Al-Mazari2Hashem Abu Hurirah3Ahed J. Alkhatib4Department of Pharmaceutical Sciences, Faculty of Pharmacy, Jadara University, Irbid, JordanFaculty of Pharmacy, Zarqa University, JordanFaculty of Pharmacy, Zarqa University, JordanFaculty of Pharmacy, Zarqa University, JordanDepartment of Legal Medicine, Toxicology of Forensic Science and Toxicology, School of Medicine, Jordan University of Science and Technology, JordanIntroduction. Diabetes is increasingly prevalent at global level and associated with various impacts including the male reproductive system. Aims. This research is aimed at investigating the influence of diabetes on the localization and expression of HSP90 and iNOS in the testicular tissue of diabetic rats. Methods. A diabetic model was developed through a single injection of alloxan monohydrate intraperitoneally (purchased from Sigma-Aldrich) 120 mg/kg body weight following fasting for 12 hrs. The experiment involved two groups, the control and diabetic groups with 10 albino rats in each group. Diabetes was considered if glucose concentration was ≥200 mg/dl. The experiment duration was for one month. After the experiment had finished, all rats were terminated and prepared for routine histological and immunohistochemical examination. Results. The results revealed that diabetes caused morphological changes at histological level in testicular tissue. Immunohistochemical examination showed that diabetes significantly upregulated the expression of both HSP90 and iNOS in the testicular tissue of diabetic rats as compared with that of the control group (p<0.001). Conclusion. Diabetes may induce adverse health effects on the male reproduction through upregulation of HSP90 and iNOS in the testicular tissue of diabetic rats.http://dx.doi.org/10.1155/2020/3906583 |
| spellingShingle | Ali Alsarhan Kawther Faisal Amawi Inas Saleh Al-Mazari Hashem Abu Hurirah Ahed J. Alkhatib The Compound Expression of HSP90 and INOS in the Testis of Diabetic Rats as Cellular and Pathologic Adverse Effects of Diabetes Analytical Cellular Pathology |
| title | The Compound Expression of HSP90 and INOS in the Testis of Diabetic Rats as Cellular and Pathologic Adverse Effects of Diabetes |
| title_full | The Compound Expression of HSP90 and INOS in the Testis of Diabetic Rats as Cellular and Pathologic Adverse Effects of Diabetes |
| title_fullStr | The Compound Expression of HSP90 and INOS in the Testis of Diabetic Rats as Cellular and Pathologic Adverse Effects of Diabetes |
| title_full_unstemmed | The Compound Expression of HSP90 and INOS in the Testis of Diabetic Rats as Cellular and Pathologic Adverse Effects of Diabetes |
| title_short | The Compound Expression of HSP90 and INOS in the Testis of Diabetic Rats as Cellular and Pathologic Adverse Effects of Diabetes |
| title_sort | compound expression of hsp90 and inos in the testis of diabetic rats as cellular and pathologic adverse effects of diabetes |
| url | http://dx.doi.org/10.1155/2020/3906583 |
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