Gibberellin-Induced Transcription Factor <i>Sm</i>MYB71 Negatively Regulates Salvianolic Acid Biosynthesis in <i>Salvia miltiorrhiza</i>

Gibberellin-Induced Transcription Factor <i>Sm</i>MYB71 Negatively Regulates Salvianolic Acid Biosynthesis in <i>Salvia miltiorrhiza</i>

<i>Salvia miltiorrhiza</i>, the valuable traditional Chinese medicinal plant, has been used in clinics for thousands of years. The water-soluble salvianolic acid compounds are bioactive substances used in treating many diseases. Gibberellins (GAs) are growth-promoting phytohormones that...

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Main Authors: Cuicui Han, Xingwen Dong, Xiaowen Xing, Yun Wang, Xiaobing Feng, Wenjuan Sang, Yifei Feng, Luyao Yu, Mengxuan Chen, Hongyuan Hao, Taohong Huang, Bailin Li, Wenhui Wu, Zheng Zhou, Ying He
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Molecules
Subjects:
salvianolic acid
<i>Sm</i>MYB71
gibberellins
<i>Sm</i>ERF115
biosynthesis
Online Access:https://www.mdpi.com/1420-3049/29/24/5892
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Summary:<i>Salvia miltiorrhiza</i>, the valuable traditional Chinese medicinal plant, has been used in clinics for thousands of years. The water-soluble salvianolic acid compounds are bioactive substances used in treating many diseases. Gibberellins (GAs) are growth-promoting phytohormones that regulate plant growth and development. Previous studies have demonstrated that GAs can promote salvianolic acid accumulation in <i>S. miltiorrhiza</i>; however, the underlying mechanism requires further investigation. Here, we identified a GA-induced R2R3MYB transcription factor (TF), <i>Sm</i>MYB71, from a transcriptome library of GA-treated <i>S. miltiorrhiza</i>. <i>Sm</i>MYB71 was highly expressed in the root of <i>S. miltiorrhiza</i> and localized to the nucleus. <i>Sm</i>MYB71-knockout hairy roots showed higher salvianolic acid accumulation compared to wild lines. Overexpressing <i>Sm</i>MYB71 in <i>S. miltiorrhiza</i> hairy roots significantly decreased the content of salvianolic acid by downregulating key salvianolic acid biosynthesis enzymes such as <i>Sm</i>RAS and <i>Sm</i>CYP98A14. The GCC box in the promoter of <i>Sm</i>MYB71 can bind with <i>Sm</i>ERF115, suggesting that <i>Sm</i>MYB71 is regulated by <i>Sm</i>ERF115 in salvianolic acid biosynthesis. These findings demonstrate a novel regulatory role of <i>Sm</i>MYB71 in GA-mediated phenolic acid biosynthesis. With the development of CRISPR/Cas9-based genome editing technology, the <i>Sm</i>MYB71 regulation mechanism of salvianolic acid biosynthesis provides a potential target gene for metabolic engineering to increase the quality of <i>S. miltiorrhiza</i>.
ISSN:1420-3049

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