Tripterygium Glycosides Tablet Ameliorates Renal Tubulointerstitial Fibrosis via the Toll-Like Receptor 4/Nuclear Factor Kappa B Signaling Pathway in High-Fat Diet Fed and Streptozotocin-Induced Diabetic Rats
Tripterygium glycosides tablet (TGT) is a Chinese traditional medicine that has been shown to protect podocytes from injury and reduce the proteinuria. The aim of this study was to assess the effect of TGT on renal tubulointerstitial fibrosis and its potential mechanism in high-fat diet fed and STZ-...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2015/390428 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551308312182784 |
|---|---|
| author | Ze-jun Ma Xiao-na Zhang Li Li Wei Yang Shan-shan Wang Xin Guo Pei Sun Li-ming Chen |
| author_facet | Ze-jun Ma Xiao-na Zhang Li Li Wei Yang Shan-shan Wang Xin Guo Pei Sun Li-ming Chen |
| author_sort | Ze-jun Ma |
| collection | DOAJ |
| description | Tripterygium glycosides tablet (TGT) is a Chinese traditional medicine that has been shown to protect podocytes from injury and reduce the proteinuria. The aim of this study was to assess the effect of TGT on renal tubulointerstitial fibrosis and its potential mechanism in high-fat diet fed and STZ-induced diabetic rats. Rats were randomly divided into normal control rats (NC group), diabetic rats without drug treatment (DM group), and diabetic rats treated with TGT (1, 3, or 6 mg/kg/day, respectively) for 8 weeks. The results showed that 24 h proteinuria and urinary N-acetyl-glucosaminidase (NAG) in diabetic rats were decreased by TGT treatment without affecting blood glucose. Masson’s trichrome stains showed that apparent renal tubulointerstitial fibrosis was found in DM group, which was ameliorated by TGT treatment. The expression of α-SMA was significantly decreased, accompanied by increased expression of E-cadherin in TGT-treated rats, but not in untreated DM rats. Further studies showed that TGT administration markedly reduced expression of TLR4, NF-κB, IL-1β, and MCP-1 in TGT-treated diabetic rats. These results showed that TGT could ameliorate renal tubulointerstitial fibrosis, the mechanism which may be at least partly associated with the amelioration of EMT through suppression of the TLR4/NF-κB pathway. |
| format | Article |
| id | doaj-art-245c9aac9e72414c973fd3eccc6fd27b |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-245c9aac9e72414c973fd3eccc6fd27b2025-02-03T06:01:48ZengWileyJournal of Diabetes Research2314-67452314-67532015-01-01201510.1155/2015/390428390428Tripterygium Glycosides Tablet Ameliorates Renal Tubulointerstitial Fibrosis via the Toll-Like Receptor 4/Nuclear Factor Kappa B Signaling Pathway in High-Fat Diet Fed and Streptozotocin-Induced Diabetic RatsZe-jun Ma0Xiao-na Zhang1Li Li2Wei Yang3Shan-shan Wang4Xin Guo5Pei Sun6Li-ming Chen72011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Key Laboratory of Hormone and Development (Ministry of Health), Metabolic Disease Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, China2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Key Laboratory of Hormone and Development (Ministry of Health), Metabolic Disease Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, China2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Key Laboratory of Hormone and Development (Ministry of Health), Metabolic Disease Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, China2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Key Laboratory of Hormone and Development (Ministry of Health), Metabolic Disease Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, China2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Key Laboratory of Hormone and Development (Ministry of Health), Metabolic Disease Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, China2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Key Laboratory of Hormone and Development (Ministry of Health), Metabolic Disease Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, China2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Key Laboratory of Hormone and Development (Ministry of Health), Metabolic Disease Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, China2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Key Laboratory of Hormone and Development (Ministry of Health), Metabolic Disease Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, ChinaTripterygium glycosides tablet (TGT) is a Chinese traditional medicine that has been shown to protect podocytes from injury and reduce the proteinuria. The aim of this study was to assess the effect of TGT on renal tubulointerstitial fibrosis and its potential mechanism in high-fat diet fed and STZ-induced diabetic rats. Rats were randomly divided into normal control rats (NC group), diabetic rats without drug treatment (DM group), and diabetic rats treated with TGT (1, 3, or 6 mg/kg/day, respectively) for 8 weeks. The results showed that 24 h proteinuria and urinary N-acetyl-glucosaminidase (NAG) in diabetic rats were decreased by TGT treatment without affecting blood glucose. Masson’s trichrome stains showed that apparent renal tubulointerstitial fibrosis was found in DM group, which was ameliorated by TGT treatment. The expression of α-SMA was significantly decreased, accompanied by increased expression of E-cadherin in TGT-treated rats, but not in untreated DM rats. Further studies showed that TGT administration markedly reduced expression of TLR4, NF-κB, IL-1β, and MCP-1 in TGT-treated diabetic rats. These results showed that TGT could ameliorate renal tubulointerstitial fibrosis, the mechanism which may be at least partly associated with the amelioration of EMT through suppression of the TLR4/NF-κB pathway.http://dx.doi.org/10.1155/2015/390428 |
| spellingShingle | Ze-jun Ma Xiao-na Zhang Li Li Wei Yang Shan-shan Wang Xin Guo Pei Sun Li-ming Chen Tripterygium Glycosides Tablet Ameliorates Renal Tubulointerstitial Fibrosis via the Toll-Like Receptor 4/Nuclear Factor Kappa B Signaling Pathway in High-Fat Diet Fed and Streptozotocin-Induced Diabetic Rats Journal of Diabetes Research |
| title | Tripterygium Glycosides Tablet Ameliorates Renal Tubulointerstitial Fibrosis via the Toll-Like Receptor 4/Nuclear Factor Kappa B Signaling Pathway in High-Fat Diet Fed and Streptozotocin-Induced Diabetic Rats |
| title_full | Tripterygium Glycosides Tablet Ameliorates Renal Tubulointerstitial Fibrosis via the Toll-Like Receptor 4/Nuclear Factor Kappa B Signaling Pathway in High-Fat Diet Fed and Streptozotocin-Induced Diabetic Rats |
| title_fullStr | Tripterygium Glycosides Tablet Ameliorates Renal Tubulointerstitial Fibrosis via the Toll-Like Receptor 4/Nuclear Factor Kappa B Signaling Pathway in High-Fat Diet Fed and Streptozotocin-Induced Diabetic Rats |
| title_full_unstemmed | Tripterygium Glycosides Tablet Ameliorates Renal Tubulointerstitial Fibrosis via the Toll-Like Receptor 4/Nuclear Factor Kappa B Signaling Pathway in High-Fat Diet Fed and Streptozotocin-Induced Diabetic Rats |
| title_short | Tripterygium Glycosides Tablet Ameliorates Renal Tubulointerstitial Fibrosis via the Toll-Like Receptor 4/Nuclear Factor Kappa B Signaling Pathway in High-Fat Diet Fed and Streptozotocin-Induced Diabetic Rats |
| title_sort | tripterygium glycosides tablet ameliorates renal tubulointerstitial fibrosis via the toll like receptor 4 nuclear factor kappa b signaling pathway in high fat diet fed and streptozotocin induced diabetic rats |
| url | http://dx.doi.org/10.1155/2015/390428 |
| work_keys_str_mv | AT zejunma tripterygiumglycosidestabletamelioratesrenaltubulointerstitialfibrosisviathetolllikereceptor4nuclearfactorkappabsignalingpathwayinhighfatdietfedandstreptozotocininduceddiabeticrats AT xiaonazhang tripterygiumglycosidestabletamelioratesrenaltubulointerstitialfibrosisviathetolllikereceptor4nuclearfactorkappabsignalingpathwayinhighfatdietfedandstreptozotocininduceddiabeticrats AT lili tripterygiumglycosidestabletamelioratesrenaltubulointerstitialfibrosisviathetolllikereceptor4nuclearfactorkappabsignalingpathwayinhighfatdietfedandstreptozotocininduceddiabeticrats AT weiyang tripterygiumglycosidestabletamelioratesrenaltubulointerstitialfibrosisviathetolllikereceptor4nuclearfactorkappabsignalingpathwayinhighfatdietfedandstreptozotocininduceddiabeticrats AT shanshanwang tripterygiumglycosidestabletamelioratesrenaltubulointerstitialfibrosisviathetolllikereceptor4nuclearfactorkappabsignalingpathwayinhighfatdietfedandstreptozotocininduceddiabeticrats AT xinguo tripterygiumglycosidestabletamelioratesrenaltubulointerstitialfibrosisviathetolllikereceptor4nuclearfactorkappabsignalingpathwayinhighfatdietfedandstreptozotocininduceddiabeticrats AT peisun tripterygiumglycosidestabletamelioratesrenaltubulointerstitialfibrosisviathetolllikereceptor4nuclearfactorkappabsignalingpathwayinhighfatdietfedandstreptozotocininduceddiabeticrats AT limingchen tripterygiumglycosidestabletamelioratesrenaltubulointerstitialfibrosisviathetolllikereceptor4nuclearfactorkappabsignalingpathwayinhighfatdietfedandstreptozotocininduceddiabeticrats |