HCV定量RT-PCR竞争性参比标准的构建
摘 要 将克隆的HCV 5’ NCR序列反向插入pS P72的Eco RV 切点, 构建一种能转录HCV -RN A的质粒 pSnc-2。以RT-PCR从抗HEV IgM阳性病人血浆中扩增一段238 bp的HCV-cDN A,反向插入pSnc-2中克隆的 5’ NC R序列的NcoⅠ 切点,构建插入突变型HCV-cDN A重组体p Snc-e。应用限制性酶切和PCR对这两种重组 质粒进行了鉴定,为HCV-RN A的定量PCR提供有用的内参照模板。...
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| Main Author: | |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Sun Yat-sen University
1997-01-01
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| Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
| Subjects: | |
| Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/43589832/ |
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