Genetic Background and Phenotypic Aspects of Light-Induced Carotenoid Biosynthesis in <i>Isosphaeraceae</i> Planctomycetes
Nearly half of the currently described planctomycetes display pink, red, or orange pigmentation. Until recently, however, carotenoid biosynthesis in these bacteria remained largely unexplored. We examined the carotenogenesis and its genetic background in a novel planctomycete of the family <i>...
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2024-12-01
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| author | Anastasia A. Ivanova Igor Y. Oshkin Aleksandr A. Ashikhmin Olga V. Danilova Svetlana N. Dedysh |
| author_facet | Anastasia A. Ivanova Igor Y. Oshkin Aleksandr A. Ashikhmin Olga V. Danilova Svetlana N. Dedysh |
| author_sort | Anastasia A. Ivanova |
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| description | Nearly half of the currently described planctomycetes display pink, red, or orange pigmentation. Until recently, however, carotenoid biosynthesis in these bacteria remained largely unexplored. We examined the carotenogenesis and its genetic background in a novel planctomycete of the family <i>Isosphaeraceae</i>, <i>Singulisphaera</i> sp. Ch08. This bacterium changed its color from white to pink when exposed to light during cultivation. Major components of the pigment extract from strain Ch08 were derivatives of the C30 carotenoid 4,4′-diapolycopene. The genes involved in the carotenoid biosynthetic pathways of strain Ch08, a non-pigmented planctomycete <i>Singulisphaera acidiphila</i> MOB10<sup>T</sup>, and all described <i>Isosphaeraceae</i> members with determined genome sequences were analyzed. The biosynthesis of C30 carotenoids via squalene, as evidenced by the presence of <i>hpnCDE</i> and <i>crtNPQO</i> gene clusters, was encoded in genomes of all pigmented <i>Singulisphaera</i>, <i>Aquisphaera</i>, and <i>Paludisphaera</i> species. Non-pigmented <i>Singulisphaera acidiphila</i> MOB10<sup>T</sup> and <i>Tautonia sociabilis</i> GM2012<sup>T</sup> lacked full sets of genes required for carotenoid biosynthesis. <i>Isosphaera pallida</i> IS1B<sup>T</sup> and pigmented <i>Tautonia</i> species possessed <i>crtB</i> and <i>crtI</i> genes, enabling production of C40 carotenoids, but the key genes associated with C30 carotenoid biosynthesis were absent. Notably, some <i>Isosphaeraceae</i> genomes, including that of <i>Singulisphaera</i> sp. Ch08, harbor the putative <i>carH</i> gene, which is involved in B<sub>12</sub>-dependent photoregulation of carotenogenesis. |
| format | Article |
| id | doaj-art-23b29b0f8c414007839c1d0a38274f71 |
| institution | Kabale University |
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| spelling | doaj-art-23b29b0f8c414007839c1d0a38274f712025-01-24T13:41:42ZengMDPI AGMicrobiology Research2036-74812024-12-01161510.3390/microbiolres16010005Genetic Background and Phenotypic Aspects of Light-Induced Carotenoid Biosynthesis in <i>Isosphaeraceae</i> PlanctomycetesAnastasia A. Ivanova0Igor Y. Oshkin1Aleksandr A. Ashikhmin2Olga V. Danilova3Svetlana N. Dedysh4Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, RussiaInstitute of Basic Biological Problems, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino 142290, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, RussiaNearly half of the currently described planctomycetes display pink, red, or orange pigmentation. Until recently, however, carotenoid biosynthesis in these bacteria remained largely unexplored. We examined the carotenogenesis and its genetic background in a novel planctomycete of the family <i>Isosphaeraceae</i>, <i>Singulisphaera</i> sp. Ch08. This bacterium changed its color from white to pink when exposed to light during cultivation. Major components of the pigment extract from strain Ch08 were derivatives of the C30 carotenoid 4,4′-diapolycopene. The genes involved in the carotenoid biosynthetic pathways of strain Ch08, a non-pigmented planctomycete <i>Singulisphaera acidiphila</i> MOB10<sup>T</sup>, and all described <i>Isosphaeraceae</i> members with determined genome sequences were analyzed. The biosynthesis of C30 carotenoids via squalene, as evidenced by the presence of <i>hpnCDE</i> and <i>crtNPQO</i> gene clusters, was encoded in genomes of all pigmented <i>Singulisphaera</i>, <i>Aquisphaera</i>, and <i>Paludisphaera</i> species. Non-pigmented <i>Singulisphaera acidiphila</i> MOB10<sup>T</sup> and <i>Tautonia sociabilis</i> GM2012<sup>T</sup> lacked full sets of genes required for carotenoid biosynthesis. <i>Isosphaera pallida</i> IS1B<sup>T</sup> and pigmented <i>Tautonia</i> species possessed <i>crtB</i> and <i>crtI</i> genes, enabling production of C40 carotenoids, but the key genes associated with C30 carotenoid biosynthesis were absent. Notably, some <i>Isosphaeraceae</i> genomes, including that of <i>Singulisphaera</i> sp. Ch08, harbor the putative <i>carH</i> gene, which is involved in B<sub>12</sub>-dependent photoregulation of carotenogenesis.https://www.mdpi.com/2036-7481/16/1/5C30 carotenoidslight-induced carotenogenesisB<sub>12</sub>-dependent carotenogenesisgenus <i>Singulisphaera</i>family <i>Isosphaeraceae</i><i>Planctomycetes</i> |
| spellingShingle | Anastasia A. Ivanova Igor Y. Oshkin Aleksandr A. Ashikhmin Olga V. Danilova Svetlana N. Dedysh Genetic Background and Phenotypic Aspects of Light-Induced Carotenoid Biosynthesis in <i>Isosphaeraceae</i> Planctomycetes Microbiology Research C30 carotenoids light-induced carotenogenesis B<sub>12</sub>-dependent carotenogenesis genus <i>Singulisphaera</i> family <i>Isosphaeraceae</i> <i>Planctomycetes</i> |
| title | Genetic Background and Phenotypic Aspects of Light-Induced Carotenoid Biosynthesis in <i>Isosphaeraceae</i> Planctomycetes |
| title_full | Genetic Background and Phenotypic Aspects of Light-Induced Carotenoid Biosynthesis in <i>Isosphaeraceae</i> Planctomycetes |
| title_fullStr | Genetic Background and Phenotypic Aspects of Light-Induced Carotenoid Biosynthesis in <i>Isosphaeraceae</i> Planctomycetes |
| title_full_unstemmed | Genetic Background and Phenotypic Aspects of Light-Induced Carotenoid Biosynthesis in <i>Isosphaeraceae</i> Planctomycetes |
| title_short | Genetic Background and Phenotypic Aspects of Light-Induced Carotenoid Biosynthesis in <i>Isosphaeraceae</i> Planctomycetes |
| title_sort | genetic background and phenotypic aspects of light induced carotenoid biosynthesis in i isosphaeraceae i planctomycetes |
| topic | C30 carotenoids light-induced carotenogenesis B<sub>12</sub>-dependent carotenogenesis genus <i>Singulisphaera</i> family <i>Isosphaeraceae</i> <i>Planctomycetes</i> |
| url | https://www.mdpi.com/2036-7481/16/1/5 |
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