A Mg2+-dependent high-yield method for extracting high-molecular-weight genomic DNA from a single planarian specimen

A Mg2+-dependent high-yield method for extracting high-molecular-weight genomic DNA from a single planarian specimen

Abstract Background The isolation of intact, high-molecular-weight genomic DNA (HMW gDNA) is essential for achieving complete genome assemblies. However, extracting HMW gDNA from a single individual of Dugesia japonica remains a technical challenge using the standard protocol, probably due to the pr...

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Bibliographic Details
Main Authors: Ao Li, Bingrui Sun, Ying Zhang, Ping Yu, Jicheng Qu, Hongkuan Deng, Qiuxiang Pang, Fengtang Yang
Format: Article
Language:English
Published: BMC 2025-05-01
Series:BMC Genomics
Subjects:
High-molecular-weight genomic DNA extraction
High yield
Planarian
Deoxyribonuclease II
Mg2+
Online Access:https://doi.org/10.1186/s12864-025-11731-6
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