Research Note: Establishment of vector system harboring duck RNA polymerase I promoter for avian influenza virus
Reverse genetics (RG) systems are extensively utilized to investigate the characteristics of influenza viruses and develop vaccines, predominantly relying on human RNA polymerase I (pol I). However, the efficiency of RG systems for avian-origin influenza viruses may be compromised due to potential s...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124011489 |
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| author | Yunyueng Jang Yoon-Gi Baek Yu-Na Lee Ra Mi Cha Yun-Chan Choi Min-Ji Park Youn-Jeong Lee Eun-Kyoung Lee |
| author_facet | Yunyueng Jang Yoon-Gi Baek Yu-Na Lee Ra Mi Cha Yun-Chan Choi Min-Ji Park Youn-Jeong Lee Eun-Kyoung Lee |
| author_sort | Yunyueng Jang |
| collection | DOAJ |
| description | Reverse genetics (RG) systems are extensively utilized to investigate the characteristics of influenza viruses and develop vaccines, predominantly relying on human RNA polymerase I (pol I). However, the efficiency of RG systems for avian-origin influenza viruses may be compromised due to potential species-specific interactions of RNA pol I. In this study, we reported the polymerase activities of the duck RNA pol I promoter in avian cells and the generation of recombinant avian-derived influenza viruses using a novel vector system containing the duck RNA pol I promoter region to enhance the rescue efficiency of the RG system in avian cells. Initially, we explored a putative duck promoter region and identified the optimal size to improve the existing system. Subsequently, we established an RG system incorporating the duck RNA pol I promoter and compared its rescue efficiency with the human pol I system by generating recombinant influenza viruses in several cell lines. Notably, the 250-bp duck RNA pol I promoter demonstrated effective functionality in avian cells, exhibiting higher polymerase activity in a minigenome assay. The newly constructed RG system was significantly improved, enabling the rescue of influenza viruses in 293T, DF-1, and CCL141 cells. Furthermore, HPAI viruses were successfully rescued in DF-1 cells, a result that had not been achieved in previous experiments. In conclusion, our novel RG system harboring duck RNA pol I offers an additional tool for researching influenza viruses and may facilitate the development of vaccines for poultry. |
| format | Article |
| id | doaj-art-22799df5de0a4695bf7f93e4d6bca7cb |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-22799df5de0a4695bf7f93e4d6bca7cb2025-01-22T05:40:31ZengElsevierPoultry Science0032-57912025-01-011041104570Research Note: Establishment of vector system harboring duck RNA polymerase I promoter for avian influenza virusYunyueng Jang0Yoon-Gi Baek1Yu-Na Lee2Ra Mi Cha3Yun-Chan Choi4Min-Ji Park5Youn-Jeong Lee6Eun-Kyoung Lee7Avian Influenza Research & Diagnostic Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Republic of KoreaAvian Influenza Research & Diagnostic Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Republic of KoreaAvian Influenza Research & Diagnostic Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Republic of KoreaAvian Influenza Research & Diagnostic Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Republic of KoreaAvian Influenza Research & Diagnostic Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Republic of KoreaAvian Influenza Research & Diagnostic Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Republic of KoreaAvian Influenza Research & Diagnostic Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Republic of KoreaCorresponding author.; Avian Influenza Research & Diagnostic Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Republic of KoreaReverse genetics (RG) systems are extensively utilized to investigate the characteristics of influenza viruses and develop vaccines, predominantly relying on human RNA polymerase I (pol I). However, the efficiency of RG systems for avian-origin influenza viruses may be compromised due to potential species-specific interactions of RNA pol I. In this study, we reported the polymerase activities of the duck RNA pol I promoter in avian cells and the generation of recombinant avian-derived influenza viruses using a novel vector system containing the duck RNA pol I promoter region to enhance the rescue efficiency of the RG system in avian cells. Initially, we explored a putative duck promoter region and identified the optimal size to improve the existing system. Subsequently, we established an RG system incorporating the duck RNA pol I promoter and compared its rescue efficiency with the human pol I system by generating recombinant influenza viruses in several cell lines. Notably, the 250-bp duck RNA pol I promoter demonstrated effective functionality in avian cells, exhibiting higher polymerase activity in a minigenome assay. The newly constructed RG system was significantly improved, enabling the rescue of influenza viruses in 293T, DF-1, and CCL141 cells. Furthermore, HPAI viruses were successfully rescued in DF-1 cells, a result that had not been achieved in previous experiments. In conclusion, our novel RG system harboring duck RNA pol I offers an additional tool for researching influenza viruses and may facilitate the development of vaccines for poultry.http://www.sciencedirect.com/science/article/pii/S0032579124011489Duck RNA polymerase IAvian influenzaReverse genetics |
| spellingShingle | Yunyueng Jang Yoon-Gi Baek Yu-Na Lee Ra Mi Cha Yun-Chan Choi Min-Ji Park Youn-Jeong Lee Eun-Kyoung Lee Research Note: Establishment of vector system harboring duck RNA polymerase I promoter for avian influenza virus Poultry Science Duck RNA polymerase I Avian influenza Reverse genetics |
| title | Research Note: Establishment of vector system harboring duck RNA polymerase I promoter for avian influenza virus |
| title_full | Research Note: Establishment of vector system harboring duck RNA polymerase I promoter for avian influenza virus |
| title_fullStr | Research Note: Establishment of vector system harboring duck RNA polymerase I promoter for avian influenza virus |
| title_full_unstemmed | Research Note: Establishment of vector system harboring duck RNA polymerase I promoter for avian influenza virus |
| title_short | Research Note: Establishment of vector system harboring duck RNA polymerase I promoter for avian influenza virus |
| title_sort | research note establishment of vector system harboring duck rna polymerase i promoter for avian influenza virus |
| topic | Duck RNA polymerase I Avian influenza Reverse genetics |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124011489 |
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