Deciphering the pseudouridine nucleobase modification in human diseases: From molecular mechanisms to clinical perspectives
Abstract RNA pseudouridylation, a dynamic and reversible post‐transcriptional modification found in diverse RNA species, is crucial for various biological processes, including tRNA homeostasis, tRNA transport, translation initiation regulation, pre‐mRNA splicing, enhancement of mRNA translation, and...
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Wiley
2025-01-01
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| Series: | Clinical and Translational Medicine |
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| Online Access: | https://doi.org/10.1002/ctm2.70190 |
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| author | Shiheng Jia Xue Yu Na Deng Chen Zheng Mingguang Ju Fanglin Wang Yixiao Zhang Ziming Gao Yanshu Li Heng Zhou Kai Li |
| author_facet | Shiheng Jia Xue Yu Na Deng Chen Zheng Mingguang Ju Fanglin Wang Yixiao Zhang Ziming Gao Yanshu Li Heng Zhou Kai Li |
| author_sort | Shiheng Jia |
| collection | DOAJ |
| description | Abstract RNA pseudouridylation, a dynamic and reversible post‐transcriptional modification found in diverse RNA species, is crucial for various biological processes, including tRNA homeostasis, tRNA transport, translation initiation regulation, pre‐mRNA splicing, enhancement of mRNA translation, and translational fidelity. Disruption of pseudouridylation impairs cellular homeostasis, contributing to pathological alterations. Recent studies have highlighted its regulatory role in human diseases, particularly in tumourigenesis. Cellular stresses trigger RNA pseudouridylation in organisms, suggesting that pseudouridylation‐mediated epigenetic reprogramming is essential for maintaining cellular viability and responding to stress. This review examines the regulatory mechanisms and pathological implications of pseudouridylation in human diseases, with a focus on its involvement in tumourigenesis. Additionally, it explores the therapeutic potential of targeting pseudouridylation, presenting novel strategies for disease treatment. Highlights Methods to detect pseudouridine were introduced from classic mass spectrometry‐based methods to newer approaches such as nanopore‐based technologies and BID sequencing, each with its advantages and limitations. RNA pseudouridylation is crucial for various biological processes, including tRNA homeostasis, tRNA transport, translation initiation regulation, pre‐mRNA splicing, enhancement of mRNA translation, and translational fidelity. Increased pseudouridylation is frequently associated with tumour initiation, progression, and poor prognosis, whereas its reduction is predominantly implicated in non‐tumour diseases. A comprehensive understanding of the inducing factors for RNA pseudouridylation will be essential for elucidating its role in diseases. Such insights can provide robust evidence for how pseudouridylation influences disease progression and offer new avenues for therapeutic strategies targeting pseudouridylation dysregulation. The therapeutic potential of RNA pseudouridylation in diseases is enormous, including inhibitors targeting pseudouridine synthases, the application of RNA pseudouridylation in RNA therapeutics, and its role as a biological marker. |
| format | Article |
| id | doaj-art-2275f0eca6dc4503bda1dae919a059e3 |
| institution | Kabale University |
| issn | 2001-1326 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Translational Medicine |
| spelling | doaj-art-2275f0eca6dc4503bda1dae919a059e32025-01-25T04:00:38ZengWileyClinical and Translational Medicine2001-13262025-01-01151n/an/a10.1002/ctm2.70190Deciphering the pseudouridine nucleobase modification in human diseases: From molecular mechanisms to clinical perspectivesShiheng Jia0Xue Yu1Na Deng2Chen Zheng3Mingguang Ju4Fanglin Wang5Yixiao Zhang6Ziming Gao7Yanshu Li8Heng Zhou9Kai Li10Department of Surgical Oncology and General Surgery The First Hospital of China Medical University Shenyang Liaoning ChinaDepartment of Surgical Oncology and General Surgery The First Hospital of China Medical University Shenyang Liaoning ChinaDepartment of Hematology The Fourth Affiliated Hospital of China Medical University Shenyang Liaoning ChinaDepartment of Surgical Oncology and General Surgery The First Hospital of China Medical University Shenyang Liaoning ChinaDepartment of Surgical Oncology and General Surgery The First Hospital of China Medical University Shenyang Liaoning ChinaDepartment of Surgical Oncology and General Surgery The First Hospital of China Medical University Shenyang Liaoning ChinaDepartment of Surgical Oncology and General Surgery The First Hospital of China Medical University Shenyang Liaoning ChinaDepartment of Surgical Oncology and General Surgery The First Hospital of China Medical University Shenyang Liaoning ChinaDepartment of Cell Biology Key Laboratory of Cell Biology National Health Commission of the PRC and Key Laboratory of Medical Cell Biology Ministry of Education of the PRC China Medical University Shenyang Liaoning ChinaDepartment of Surgical Oncology and General Surgery The First Hospital of China Medical University Shenyang Liaoning ChinaDepartment of Surgical Oncology and General Surgery The First Hospital of China Medical University Shenyang Liaoning ChinaAbstract RNA pseudouridylation, a dynamic and reversible post‐transcriptional modification found in diverse RNA species, is crucial for various biological processes, including tRNA homeostasis, tRNA transport, translation initiation regulation, pre‐mRNA splicing, enhancement of mRNA translation, and translational fidelity. Disruption of pseudouridylation impairs cellular homeostasis, contributing to pathological alterations. Recent studies have highlighted its regulatory role in human diseases, particularly in tumourigenesis. Cellular stresses trigger RNA pseudouridylation in organisms, suggesting that pseudouridylation‐mediated epigenetic reprogramming is essential for maintaining cellular viability and responding to stress. This review examines the regulatory mechanisms and pathological implications of pseudouridylation in human diseases, with a focus on its involvement in tumourigenesis. Additionally, it explores the therapeutic potential of targeting pseudouridylation, presenting novel strategies for disease treatment. Highlights Methods to detect pseudouridine were introduced from classic mass spectrometry‐based methods to newer approaches such as nanopore‐based technologies and BID sequencing, each with its advantages and limitations. RNA pseudouridylation is crucial for various biological processes, including tRNA homeostasis, tRNA transport, translation initiation regulation, pre‐mRNA splicing, enhancement of mRNA translation, and translational fidelity. Increased pseudouridylation is frequently associated with tumour initiation, progression, and poor prognosis, whereas its reduction is predominantly implicated in non‐tumour diseases. A comprehensive understanding of the inducing factors for RNA pseudouridylation will be essential for elucidating its role in diseases. Such insights can provide robust evidence for how pseudouridylation influences disease progression and offer new avenues for therapeutic strategies targeting pseudouridylation dysregulation. The therapeutic potential of RNA pseudouridylation in diseases is enormous, including inhibitors targeting pseudouridine synthases, the application of RNA pseudouridylation in RNA therapeutics, and its role as a biological marker.https://doi.org/10.1002/ctm2.70190cancerdiseasepost‐transcriptional modificationpseudouridylation |
| spellingShingle | Shiheng Jia Xue Yu Na Deng Chen Zheng Mingguang Ju Fanglin Wang Yixiao Zhang Ziming Gao Yanshu Li Heng Zhou Kai Li Deciphering the pseudouridine nucleobase modification in human diseases: From molecular mechanisms to clinical perspectives Clinical and Translational Medicine cancer disease post‐transcriptional modification pseudouridylation |
| title | Deciphering the pseudouridine nucleobase modification in human diseases: From molecular mechanisms to clinical perspectives |
| title_full | Deciphering the pseudouridine nucleobase modification in human diseases: From molecular mechanisms to clinical perspectives |
| title_fullStr | Deciphering the pseudouridine nucleobase modification in human diseases: From molecular mechanisms to clinical perspectives |
| title_full_unstemmed | Deciphering the pseudouridine nucleobase modification in human diseases: From molecular mechanisms to clinical perspectives |
| title_short | Deciphering the pseudouridine nucleobase modification in human diseases: From molecular mechanisms to clinical perspectives |
| title_sort | deciphering the pseudouridine nucleobase modification in human diseases from molecular mechanisms to clinical perspectives |
| topic | cancer disease post‐transcriptional modification pseudouridylation |
| url | https://doi.org/10.1002/ctm2.70190 |
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