Protocol for assessing T cell receptor-mediated human T cell cytotoxicity

Summary: Measuring the cytotoxicity of human T cells is a valuable but challenging task. We present a protocol for assessing the ability of polyclonal human T cells to kill target cells upon T cell receptor (TCR) triggering. We describe steps for preparing L and T cells for the assay and incubation...

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Bibliographic Details
Main Authors: Kathryne E. Marks, Ifeoluwakiisi Adejoorin, John Sowerby, Deepak A. Rao
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724007068
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