The haploid induction ability analysis of various mutation of OsMATL and OsDMPs in rice
Abstract Background The high-frequency induction rate of haploid is crucial for double haploid (DH) breeding. The combination of multiple haploid-induced genes, such as ZmPLA1/MATL/NLD and ZmDMP, can synergistically enhance the haploid induction rate (HIR) in maize. However, the potential synergisti...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s12915-025-02140-y |
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| author | Siyi Liang Qin Wen Wenyu Lu Guili Yang Yingyun Yao Hairong Cai Jiafeng Wang Ming Huang Hui Wang Tao Guo |
| author_facet | Siyi Liang Qin Wen Wenyu Lu Guili Yang Yingyun Yao Hairong Cai Jiafeng Wang Ming Huang Hui Wang Tao Guo |
| author_sort | Siyi Liang |
| collection | DOAJ |
| description | Abstract Background The high-frequency induction rate of haploid is crucial for double haploid (DH) breeding. The combination of multiple haploid-induced genes, such as ZmPLA1/MATL/NLD and ZmDMP, can synergistically enhance the haploid induction rate (HIR) in maize. However, the potential synergistic effects between OsMATL and OsDMP genes in rice remain unclear. Results Knocking out OsMATL in both HuaHang No.48 (HH48) and Nipponbare (NIP) cultivars resulted in reduced seed setting rate (SSR) and haploid induction (HI). Notably, in this study, the HI capacity of OsMATL knockout mutants in indica rice surpassed that of japonica rice knockout mutants, with the proton active site in the third exon exhibiting a higher HIR compared to the first and fourth exons. Furthermore, when OsDMP1 or OsDMP3 was combined with OsMATL, they increased HIR, and an antagonistic relationship was observed between HIR and SSR in HH48 matl4dmp1 and HH48 matl4dmp3 . Conclusions In rice, the proton active site in the third exon of OsMATL exhibited higher induction efficiency, and OsDMP1 or OsDMP3 exerted a synergistic effect with OsMATL. These findings provide a foundation for further research on DH breeding in rice. |
| format | Article |
| id | doaj-art-2201fc3f647c45b6b98ebb463577fdf9 |
| institution | Kabale University |
| issn | 1741-7007 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biology |
| spelling | doaj-art-2201fc3f647c45b6b98ebb463577fdf92025-02-02T12:42:38ZengBMCBMC Biology1741-70072025-01-0123111210.1186/s12915-025-02140-yThe haploid induction ability analysis of various mutation of OsMATL and OsDMPs in riceSiyi Liang0Qin Wen1Wenyu Lu2Guili Yang3Yingyun Yao4Hairong Cai5Jiafeng Wang6Ming Huang7Hui Wang8Tao Guo9National Engineering Research Center of Plant Space Breeding, South China Agricultural UniversityThe People’s Government of Leidashi Town, Tianyuan DistrictNational Engineering Research Center of Plant Space Breeding, South China Agricultural UniversityNational Engineering Research Center of Plant Space Breeding, South China Agricultural UniversityNational Engineering Research Center of Plant Space Breeding, South China Agricultural UniversityNational Engineering Research Center of Plant Space Breeding, South China Agricultural UniversityNational Engineering Research Center of Plant Space Breeding, South China Agricultural UniversityNational Engineering Research Center of Plant Space Breeding, South China Agricultural UniversityNational Engineering Research Center of Plant Space Breeding, South China Agricultural UniversityNational Engineering Research Center of Plant Space Breeding, South China Agricultural UniversityAbstract Background The high-frequency induction rate of haploid is crucial for double haploid (DH) breeding. The combination of multiple haploid-induced genes, such as ZmPLA1/MATL/NLD and ZmDMP, can synergistically enhance the haploid induction rate (HIR) in maize. However, the potential synergistic effects between OsMATL and OsDMP genes in rice remain unclear. Results Knocking out OsMATL in both HuaHang No.48 (HH48) and Nipponbare (NIP) cultivars resulted in reduced seed setting rate (SSR) and haploid induction (HI). Notably, in this study, the HI capacity of OsMATL knockout mutants in indica rice surpassed that of japonica rice knockout mutants, with the proton active site in the third exon exhibiting a higher HIR compared to the first and fourth exons. Furthermore, when OsDMP1 or OsDMP3 was combined with OsMATL, they increased HIR, and an antagonistic relationship was observed between HIR and SSR in HH48 matl4dmp1 and HH48 matl4dmp3 . Conclusions In rice, the proton active site in the third exon of OsMATL exhibited higher induction efficiency, and OsDMP1 or OsDMP3 exerted a synergistic effect with OsMATL. These findings provide a foundation for further research on DH breeding in rice.https://doi.org/10.1186/s12915-025-02140-yRiceHaploid inductionDouble haploid breedingOsMATLOsDMPs |
| spellingShingle | Siyi Liang Qin Wen Wenyu Lu Guili Yang Yingyun Yao Hairong Cai Jiafeng Wang Ming Huang Hui Wang Tao Guo The haploid induction ability analysis of various mutation of OsMATL and OsDMPs in rice BMC Biology Rice Haploid induction Double haploid breeding OsMATL OsDMPs |
| title | The haploid induction ability analysis of various mutation of OsMATL and OsDMPs in rice |
| title_full | The haploid induction ability analysis of various mutation of OsMATL and OsDMPs in rice |
| title_fullStr | The haploid induction ability analysis of various mutation of OsMATL and OsDMPs in rice |
| title_full_unstemmed | The haploid induction ability analysis of various mutation of OsMATL and OsDMPs in rice |
| title_short | The haploid induction ability analysis of various mutation of OsMATL and OsDMPs in rice |
| title_sort | haploid induction ability analysis of various mutation of osmatl and osdmps in rice |
| topic | Rice Haploid induction Double haploid breeding OsMATL OsDMPs |
| url | https://doi.org/10.1186/s12915-025-02140-y |
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