Mitigation of the effect of variability in digital PCR assays through use of duplexed reference assays for normalization
Digital PCR has been promoted as a technique for obtaining absolute measures of the amount of nucleic acid target sequence in a sample, but still lacks standardization in data reporting. The initial method of representing data as copies per microliter produced inconsistent results and made inter-ass...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2018-08-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/btn-2018-0058 |
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