Lipidomics-based association study reveals genomic signatures of anti-cancer qualities of pigmented rice sprouts
IntroductionThe genetic wealth present in pigmented rice varieties offer abundant variation in different sources of antioxidants to meet nutritional security targets among rice-consuming communities. There is limited knowledge of the dynamic changes in the lipidome of rice during germination and the...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1533442/full |
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| author | Rhowell Navarro Tiozon Rhowell Navarro Tiozon Erstelle Pasion-Uy Saleh Alseekh Saleh Alseekh Kristel June D. Sartagoda Shem Gempesaw Joel H. G. Tolentino Alisdair R. Fernie Alisdair R. Fernie Nese Sreenivasulu |
| author_facet | Rhowell Navarro Tiozon Rhowell Navarro Tiozon Erstelle Pasion-Uy Saleh Alseekh Saleh Alseekh Kristel June D. Sartagoda Shem Gempesaw Joel H. G. Tolentino Alisdair R. Fernie Alisdair R. Fernie Nese Sreenivasulu |
| author_sort | Rhowell Navarro Tiozon |
| collection | DOAJ |
| description | IntroductionThe genetic wealth present in pigmented rice varieties offer abundant variation in different sources of antioxidants to meet nutritional security targets among rice-consuming communities. There is limited knowledge of the dynamic changes in the lipidome of rice during germination and the corresponding genes associated with the antioxidant and anti-cancerous properties of lipophilic fractions of pigmented rice sprouts (PRS).MethodsIn this study, we profiled the lipidome of diverse pigmented rice collections of germinated sprouts. Further, we employed Genome-wide association studies (GWAS), gene-set analysis, and targeted association analysis to identify the candidate genes linked to these lipids.ResultsThe genetic analyses revealed 72 candidate genes involved in the regulation of these accumulating lipids in PRS. Marker trait associations (MTA) analysis shown that the combination GGTAAC/ACAAGCTGGGCCC was associated with increased levels of unsaturated lipids and carotenoids, which likely underlie these beneficial effects. This superior MTA combination exhibited potent inhibitory activity against HCT116 and A549 cell lines, with average 1/IC50 values of 0.03 and 0.02 (mL/μg), respectively, compared to the inferior MTAs.DiscussionCollectively, our findings demonstrate that MTAs linked to selected GDSL esterase/lipase (GELP) genes, OsACP1, and lecithin-cholesterol acyltransferase significantly enhance antioxidant and anti-cancer properties, potentially through the mobilization of unsaturated lipids and carotenoids during germination. This study offers valuable insights into the health-promoting potential of germinated rice sprouts as a rich dietary source of antioxidants beneficial to human health. |
| format | Article |
| id | doaj-art-2092c2bfb49e43daa08bafa9cd5cd06f |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-2092c2bfb49e43daa08bafa9cd5cd06f2025-01-28T06:41:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011610.3389/fpls.2025.15334421533442Lipidomics-based association study reveals genomic signatures of anti-cancer qualities of pigmented rice sproutsRhowell Navarro Tiozon0Rhowell Navarro Tiozon1Erstelle Pasion-Uy2Saleh Alseekh3Saleh Alseekh4Kristel June D. Sartagoda5Shem Gempesaw6Joel H. G. Tolentino7Alisdair R. Fernie8Alisdair R. Fernie9Nese Sreenivasulu10Consumer-driven Grain Quality and Nutrition Center, Strategic Innovation Platform, International Rice Research Institute, Los Baños, PhilippinesMax-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, GermanyConsumer-driven Grain Quality and Nutrition Center, Strategic Innovation Platform, International Rice Research Institute, Los Baños, PhilippinesMax-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, GermanyCenter of Plant Systems Biology and Biotechnology, Plovdiv, BulgariaConsumer-driven Grain Quality and Nutrition Center, Strategic Innovation Platform, International Rice Research Institute, Los Baños, PhilippinesDepartment of Food Science and Chemistry, College of Science and Mathematics, University of the Philippines Mindanao, Davao City, PhilippinesDepartment of Food Science and Chemistry, College of Science and Mathematics, University of the Philippines Mindanao, Davao City, PhilippinesMax-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, GermanyCenter of Plant Systems Biology and Biotechnology, Plovdiv, BulgariaConsumer-driven Grain Quality and Nutrition Center, Strategic Innovation Platform, International Rice Research Institute, Los Baños, PhilippinesIntroductionThe genetic wealth present in pigmented rice varieties offer abundant variation in different sources of antioxidants to meet nutritional security targets among rice-consuming communities. There is limited knowledge of the dynamic changes in the lipidome of rice during germination and the corresponding genes associated with the antioxidant and anti-cancerous properties of lipophilic fractions of pigmented rice sprouts (PRS).MethodsIn this study, we profiled the lipidome of diverse pigmented rice collections of germinated sprouts. Further, we employed Genome-wide association studies (GWAS), gene-set analysis, and targeted association analysis to identify the candidate genes linked to these lipids.ResultsThe genetic analyses revealed 72 candidate genes involved in the regulation of these accumulating lipids in PRS. Marker trait associations (MTA) analysis shown that the combination GGTAAC/ACAAGCTGGGCCC was associated with increased levels of unsaturated lipids and carotenoids, which likely underlie these beneficial effects. This superior MTA combination exhibited potent inhibitory activity against HCT116 and A549 cell lines, with average 1/IC50 values of 0.03 and 0.02 (mL/μg), respectively, compared to the inferior MTAs.DiscussionCollectively, our findings demonstrate that MTAs linked to selected GDSL esterase/lipase (GELP) genes, OsACP1, and lecithin-cholesterol acyltransferase significantly enhance antioxidant and anti-cancer properties, potentially through the mobilization of unsaturated lipids and carotenoids during germination. This study offers valuable insights into the health-promoting potential of germinated rice sprouts as a rich dietary source of antioxidants beneficial to human health.https://www.frontiersin.org/articles/10.3389/fpls.2025.1533442/fullantioxidantsanticancerlipidomicslipasepigmented rice |
| spellingShingle | Rhowell Navarro Tiozon Rhowell Navarro Tiozon Erstelle Pasion-Uy Saleh Alseekh Saleh Alseekh Kristel June D. Sartagoda Shem Gempesaw Joel H. G. Tolentino Alisdair R. Fernie Alisdair R. Fernie Nese Sreenivasulu Lipidomics-based association study reveals genomic signatures of anti-cancer qualities of pigmented rice sprouts Frontiers in Plant Science antioxidants anticancer lipidomics lipase pigmented rice |
| title | Lipidomics-based association study reveals genomic signatures of anti-cancer qualities of pigmented rice sprouts |
| title_full | Lipidomics-based association study reveals genomic signatures of anti-cancer qualities of pigmented rice sprouts |
| title_fullStr | Lipidomics-based association study reveals genomic signatures of anti-cancer qualities of pigmented rice sprouts |
| title_full_unstemmed | Lipidomics-based association study reveals genomic signatures of anti-cancer qualities of pigmented rice sprouts |
| title_short | Lipidomics-based association study reveals genomic signatures of anti-cancer qualities of pigmented rice sprouts |
| title_sort | lipidomics based association study reveals genomic signatures of anti cancer qualities of pigmented rice sprouts |
| topic | antioxidants anticancer lipidomics lipase pigmented rice |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1533442/full |
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