Unhealthy Phenotype as Indicated by Salivary Biomarkers: Glucose, Insulin, VEGF-A, and IL-12p70 in Obese Kuwaiti Adolescents
Objective. Here, we investigated the relationships between obesity and the salivary concentrations of insulin, glucose, and 20 metabolic biomarkers in Kuwaiti adolescents. Previously, we have shown that certain salivary metabolic markers can act as surrogates for blood concentrations. Methods. Saliv...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Obesity |
| Online Access: | http://dx.doi.org/10.1155/2016/6860240 |
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| author | Mor-Li Hartman J. Max Goodson Ping Shi Jorel Vargas Tina Yaskell Danielle Stephens Maryann Cugini Hatice Hasturk Roula Barake Osama Alsmadi Sabiha Al-Mutawa Jitendra Ariga Pramod Soparkar Jawad Behbehani Kazem Behbehani Francine Welty |
| author_facet | Mor-Li Hartman J. Max Goodson Ping Shi Jorel Vargas Tina Yaskell Danielle Stephens Maryann Cugini Hatice Hasturk Roula Barake Osama Alsmadi Sabiha Al-Mutawa Jitendra Ariga Pramod Soparkar Jawad Behbehani Kazem Behbehani Francine Welty |
| author_sort | Mor-Li Hartman |
| collection | DOAJ |
| description | Objective. Here, we investigated the relationships between obesity and the salivary concentrations of insulin, glucose, and 20 metabolic biomarkers in Kuwaiti adolescents. Previously, we have shown that certain salivary metabolic markers can act as surrogates for blood concentrations. Methods. Salivary samples of whole saliva were collected from 8,317 adolescents. Salivary glucose concentration was measured by a high-sensitivity glucose oxidase method implemented on a robotic chemical analyzer. The concentration of salivary insulin and 20 other metabolic biomarkers was assayed in 744 randomly selected saliva samples by multiplexed bead-based immunoassay. Results. Obesity was seen in 26.5% of the adolescents. Salivary insulin predicting hyperinsulinemia occurred in 4.3% of normal-weight adolescents, 8.3% of overweight adolescents, and 25.7% of obese adolescents (p<0.0001). Salivary glucose predicting hyperglycemia was found in only 3% of obese children and was not predictive (p=0.89). Elevated salivary glucose and insulin occurring together was associated with elevated vascular endothelial growth factor and reduced salivary interleukin-12. Conclusion. Considering the surrogate nature of salivary insulin and glucose, this study suggests that elevated insulin may be a dominant sign of metabolic disease in adolescent populations. It also appears that a proangiogenic environment may accompany elevated glucose in obese adolescents. |
| format | Article |
| id | doaj-art-1ffb954ab6ac45b7b5607666645b16fe |
| institution | Kabale University |
| issn | 2090-0708 2090-0716 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Obesity |
| spelling | doaj-art-1ffb954ab6ac45b7b5607666645b16fe2025-02-03T01:11:03ZengWileyJournal of Obesity2090-07082090-07162016-01-01201610.1155/2016/68602406860240Unhealthy Phenotype as Indicated by Salivary Biomarkers: Glucose, Insulin, VEGF-A, and IL-12p70 in Obese Kuwaiti AdolescentsMor-Li Hartman0J. Max Goodson1Ping Shi2Jorel Vargas3Tina Yaskell4Danielle Stephens5Maryann Cugini6Hatice Hasturk7Roula Barake8Osama Alsmadi9Sabiha Al-Mutawa10Jitendra Ariga11Pramod Soparkar12Jawad Behbehani13Kazem Behbehani14Francine Welty15Department of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA 02142, USADepartment of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA 02142, USADepartment of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA 02142, USADepartment of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA 02142, USADepartment of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA 02142, USADepartment of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA 02142, USADepartment of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA 02142, USADepartment of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA 02142, USADepartment of Nutrition, Dasman Diabetes Institute, 15462 Dasman, KuwaitGenome Center, The Dasman Diabetes Institute, 15462 Dasman, KuwaitMinistry of Health, 13001 Safat, KuwaitMinistry of Health, 13001 Safat, KuwaitDepartment of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA 02142, USAFaculty of Dentistry, Kuwait University, 13060 Safat, KuwaitDasman Diabetes Institute, 15462 Dasman, KuwaitDivision of Cardiology, Beth Israel Deaconess Medical Center, Boston, MA 02215, USAObjective. Here, we investigated the relationships between obesity and the salivary concentrations of insulin, glucose, and 20 metabolic biomarkers in Kuwaiti adolescents. Previously, we have shown that certain salivary metabolic markers can act as surrogates for blood concentrations. Methods. Salivary samples of whole saliva were collected from 8,317 adolescents. Salivary glucose concentration was measured by a high-sensitivity glucose oxidase method implemented on a robotic chemical analyzer. The concentration of salivary insulin and 20 other metabolic biomarkers was assayed in 744 randomly selected saliva samples by multiplexed bead-based immunoassay. Results. Obesity was seen in 26.5% of the adolescents. Salivary insulin predicting hyperinsulinemia occurred in 4.3% of normal-weight adolescents, 8.3% of overweight adolescents, and 25.7% of obese adolescents (p<0.0001). Salivary glucose predicting hyperglycemia was found in only 3% of obese children and was not predictive (p=0.89). Elevated salivary glucose and insulin occurring together was associated with elevated vascular endothelial growth factor and reduced salivary interleukin-12. Conclusion. Considering the surrogate nature of salivary insulin and glucose, this study suggests that elevated insulin may be a dominant sign of metabolic disease in adolescent populations. It also appears that a proangiogenic environment may accompany elevated glucose in obese adolescents.http://dx.doi.org/10.1155/2016/6860240 |
| spellingShingle | Mor-Li Hartman J. Max Goodson Ping Shi Jorel Vargas Tina Yaskell Danielle Stephens Maryann Cugini Hatice Hasturk Roula Barake Osama Alsmadi Sabiha Al-Mutawa Jitendra Ariga Pramod Soparkar Jawad Behbehani Kazem Behbehani Francine Welty Unhealthy Phenotype as Indicated by Salivary Biomarkers: Glucose, Insulin, VEGF-A, and IL-12p70 in Obese Kuwaiti Adolescents Journal of Obesity |
| title | Unhealthy Phenotype as Indicated by Salivary Biomarkers: Glucose, Insulin, VEGF-A, and IL-12p70 in Obese Kuwaiti Adolescents |
| title_full | Unhealthy Phenotype as Indicated by Salivary Biomarkers: Glucose, Insulin, VEGF-A, and IL-12p70 in Obese Kuwaiti Adolescents |
| title_fullStr | Unhealthy Phenotype as Indicated by Salivary Biomarkers: Glucose, Insulin, VEGF-A, and IL-12p70 in Obese Kuwaiti Adolescents |
| title_full_unstemmed | Unhealthy Phenotype as Indicated by Salivary Biomarkers: Glucose, Insulin, VEGF-A, and IL-12p70 in Obese Kuwaiti Adolescents |
| title_short | Unhealthy Phenotype as Indicated by Salivary Biomarkers: Glucose, Insulin, VEGF-A, and IL-12p70 in Obese Kuwaiti Adolescents |
| title_sort | unhealthy phenotype as indicated by salivary biomarkers glucose insulin vegf a and il 12p70 in obese kuwaiti adolescents |
| url | http://dx.doi.org/10.1155/2016/6860240 |
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