Systematic qualitative proteome-wide analysis of lysine malonylation profiling in Platycodon grandiflorus
Abstract In recent years, it was found that lysine malonylation modification can affect biological metabolism and play an important role in plant life activities. Platycodon grandiflorus, an economic crop and medicinal plant, had no reports on malonylation in the related literature. This study quali...
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Springer
2025-01-01
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| Series: | Amino Acids |
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| Online Access: | https://doi.org/10.1007/s00726-024-03432-3 |
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| author | Qingshan Yang Shaowei Xu Weimin Jiang Fei Meng Shuting Wang Zongping Sun Na Chen Daiyin Peng Juan Liu Shihai Xing |
| author_facet | Qingshan Yang Shaowei Xu Weimin Jiang Fei Meng Shuting Wang Zongping Sun Na Chen Daiyin Peng Juan Liu Shihai Xing |
| author_sort | Qingshan Yang |
| collection | DOAJ |
| description | Abstract In recent years, it was found that lysine malonylation modification can affect biological metabolism and play an important role in plant life activities. Platycodon grandiflorus, an economic crop and medicinal plant, had no reports on malonylation in the related literature. This study qualitatively introduces lysine malonylation in P. grandiflorus. A total of 888 lysine malonylation-modified proteins in P. grandiflorus were identified, with a total of 1755 modification sites. According to the functional annotation, malonylated proteins were closely related to catalysis, binding, and other reactions. Subcellular localization showed that related proteins were enriched in chloroplasts, cytoplasm, and nuclei, indicating that this modification could regulate various metabolic processes. Motif analysis showed the enrichment of Alanine (A), Cysteine (C), Glycine (G), and Valine (V) amino acids surrounding malonylated lysine residues. Metabolic pathway and protein-protein interaction network analyses suggested these modifications are mainly involved in plant photosynthesis. Moreover, malonylated proteins are also involved in stress and defense responses. This study shows that lysine malonylation can affect a variety of biological processes and metabolic pathways, and the contents are reported for the first time in P. grandiflorus, which can provide important information for further research on P. grandiflorus and lysine malonylation’s role in environment stress, photosynthesis, and secondary metabolites enrichment. |
| format | Article |
| id | doaj-art-1ef0f4fea53e4a76a800ee0717a5d5e1 |
| institution | Kabale University |
| issn | 1438-2199 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Amino Acids |
| spelling | doaj-art-1ef0f4fea53e4a76a800ee0717a5d5e12025-01-19T12:28:17ZengSpringerAmino Acids1438-21992025-01-0157111410.1007/s00726-024-03432-3Systematic qualitative proteome-wide analysis of lysine malonylation profiling in Platycodon grandiflorusQingshan Yang0Shaowei Xu1Weimin Jiang2Fei Meng3Shuting Wang4Zongping Sun5Na Chen6Daiyin Peng7Juan Liu8Shihai Xing9College of Pharmacy, Anhui University of Chinese MedicineCollege of Pharmacy, Anhui University of Chinese MedicineHunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, College of Life Sciences and Environment, Hengyang Normal UniversityCollege of Pharmacy, Anhui University of Chinese MedicineCollege of Pharmacy, Anhui University of Chinese MedicineEngineering Technology Research Center of Anti-Aging, Chinese Herbal Medicine, Fuyang Normal UniversityJoint Research Center for Chinese Herbal Medicine of Anhui of IHM, Hefei Comprehensive National Science CenterCollege of Pharmacy, Anhui University of Chinese MedicineCollege of Pharmacy, Anhui University of Chinese MedicineCollege of Pharmacy, Anhui University of Chinese MedicineAbstract In recent years, it was found that lysine malonylation modification can affect biological metabolism and play an important role in plant life activities. Platycodon grandiflorus, an economic crop and medicinal plant, had no reports on malonylation in the related literature. This study qualitatively introduces lysine malonylation in P. grandiflorus. A total of 888 lysine malonylation-modified proteins in P. grandiflorus were identified, with a total of 1755 modification sites. According to the functional annotation, malonylated proteins were closely related to catalysis, binding, and other reactions. Subcellular localization showed that related proteins were enriched in chloroplasts, cytoplasm, and nuclei, indicating that this modification could regulate various metabolic processes. Motif analysis showed the enrichment of Alanine (A), Cysteine (C), Glycine (G), and Valine (V) amino acids surrounding malonylated lysine residues. Metabolic pathway and protein-protein interaction network analyses suggested these modifications are mainly involved in plant photosynthesis. Moreover, malonylated proteins are also involved in stress and defense responses. This study shows that lysine malonylation can affect a variety of biological processes and metabolic pathways, and the contents are reported for the first time in P. grandiflorus, which can provide important information for further research on P. grandiflorus and lysine malonylation’s role in environment stress, photosynthesis, and secondary metabolites enrichment.https://doi.org/10.1007/s00726-024-03432-3Platycodon grandiflorusLysine malonylationSaponin biosynthesisPolysaccharide biosynthesisHeavily-malonylated |
| spellingShingle | Qingshan Yang Shaowei Xu Weimin Jiang Fei Meng Shuting Wang Zongping Sun Na Chen Daiyin Peng Juan Liu Shihai Xing Systematic qualitative proteome-wide analysis of lysine malonylation profiling in Platycodon grandiflorus Amino Acids Platycodon grandiflorus Lysine malonylation Saponin biosynthesis Polysaccharide biosynthesis Heavily-malonylated |
| title | Systematic qualitative proteome-wide analysis of lysine malonylation profiling in Platycodon grandiflorus |
| title_full | Systematic qualitative proteome-wide analysis of lysine malonylation profiling in Platycodon grandiflorus |
| title_fullStr | Systematic qualitative proteome-wide analysis of lysine malonylation profiling in Platycodon grandiflorus |
| title_full_unstemmed | Systematic qualitative proteome-wide analysis of lysine malonylation profiling in Platycodon grandiflorus |
| title_short | Systematic qualitative proteome-wide analysis of lysine malonylation profiling in Platycodon grandiflorus |
| title_sort | systematic qualitative proteome wide analysis of lysine malonylation profiling in platycodon grandiflorus |
| topic | Platycodon grandiflorus Lysine malonylation Saponin biosynthesis Polysaccharide biosynthesis Heavily-malonylated |
| url | https://doi.org/10.1007/s00726-024-03432-3 |
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