Peroxidase-like activity of citrate-functionalized magnetite nanoparticles augmented its antibacterial potency
The classic utilization of nanomaterials has now transformed to serve enzyme-like activities (nanozymes) that found different applications, particularly as antibacterial agents. Here, we report the synthesis of citrate-functionalized iron oxide nanoparticles (IOMNPs) and the subsequent utilization o...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715625000281 |
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| author | Haitham Al-Madhagi Abd Alraouf Tarabishi Fatima Al-Zahra Hamsho Zaher Tahan Lama Alchab Wassim Abdelwahed |
| author_facet | Haitham Al-Madhagi Abd Alraouf Tarabishi Fatima Al-Zahra Hamsho Zaher Tahan Lama Alchab Wassim Abdelwahed |
| author_sort | Haitham Al-Madhagi |
| collection | DOAJ |
| description | The classic utilization of nanomaterials has now transformed to serve enzyme-like activities (nanozymes) that found different applications, particularly as antibacterial agents. Here, we report the synthesis of citrate-functionalized iron oxide nanoparticles (IOMNPs) and the subsequent utilization of its peroxidase-like activity as antibacterial agent. The nanoparticles were prepared using coprecipitation method and characterized using UV–visible spectrophotometry, Fourier-transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), zeta potential, magnetism and atomic force microscopy (AFM). The catalytic activity was assessed by its ability to oxidize pyrogallol. Finally, this nanozyme properties were harnessed for potential antibacterial potency. Gram-positive bacterium Staphylococcus aureus, and Gram-negative bacterium, Escherichia coli, using the Kirby-Bauer disk diffusion method. DLS analysis demonstrated a diameter of 93 nm of the synthesized nanoparticles and the structure revealed by AFM proved the nanooval appearance. The prepared nanozymes exhibited a zeta potential of −27.8 mV, strong magnetic behavior, and prominent peroxidase-like activity. Their testing as antibacterial agents showed enhanced efficacy when hydrogen peroxide was introduced at a volumetric ratio of 25 µL:75 µL against both strains. In conclusion, citrate-decorated IOMNPs deserves further examination on antibiotic resistant strains and subsequent in vivo models as a novel antibacterial agent. |
| format | Article |
| id | doaj-art-1e96a1f6697348afa6a3a198b3eafb92 |
| institution | Kabale University |
| issn | 2211-7156 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj-art-1e96a1f6697348afa6a3a198b3eafb922025-01-29T05:01:07ZengElsevierResults in Chemistry2211-71562025-01-0113102045Peroxidase-like activity of citrate-functionalized magnetite nanoparticles augmented its antibacterial potencyHaitham Al-Madhagi0Abd Alraouf Tarabishi1Fatima Al-Zahra Hamsho2Zaher Tahan3Lama Alchab4Wassim Abdelwahed5Biochemical Technology Program, Faculty of Applied Sciences, Dhamar University, Yemen; Corresponding author.Chemistry Department, Faculty of Sciences, Aleppo University, Syrian Arab RepublicMicrobiology Department, Faculty of Sciences, Aleppo University, Syrian Arab RepublicMicrobiology Department, Faculty of Sciences, Aleppo University, Syrian Arab RepublicPhysics Department, Faculty of Sciences, Aleppo University, Syrian Arab RepublicPharmaceutics and Pharmaceutical Technology Department, Faculty of Pharmacy, Aleppo University, Syrian Arab RepublicThe classic utilization of nanomaterials has now transformed to serve enzyme-like activities (nanozymes) that found different applications, particularly as antibacterial agents. Here, we report the synthesis of citrate-functionalized iron oxide nanoparticles (IOMNPs) and the subsequent utilization of its peroxidase-like activity as antibacterial agent. The nanoparticles were prepared using coprecipitation method and characterized using UV–visible spectrophotometry, Fourier-transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), zeta potential, magnetism and atomic force microscopy (AFM). The catalytic activity was assessed by its ability to oxidize pyrogallol. Finally, this nanozyme properties were harnessed for potential antibacterial potency. Gram-positive bacterium Staphylococcus aureus, and Gram-negative bacterium, Escherichia coli, using the Kirby-Bauer disk diffusion method. DLS analysis demonstrated a diameter of 93 nm of the synthesized nanoparticles and the structure revealed by AFM proved the nanooval appearance. The prepared nanozymes exhibited a zeta potential of −27.8 mV, strong magnetic behavior, and prominent peroxidase-like activity. Their testing as antibacterial agents showed enhanced efficacy when hydrogen peroxide was introduced at a volumetric ratio of 25 µL:75 µL against both strains. In conclusion, citrate-decorated IOMNPs deserves further examination on antibiotic resistant strains and subsequent in vivo models as a novel antibacterial agent.http://www.sciencedirect.com/science/article/pii/S2211715625000281NanotechnologyIron oxide nanoparticlesNanozymeAntibacterial agents |
| spellingShingle | Haitham Al-Madhagi Abd Alraouf Tarabishi Fatima Al-Zahra Hamsho Zaher Tahan Lama Alchab Wassim Abdelwahed Peroxidase-like activity of citrate-functionalized magnetite nanoparticles augmented its antibacterial potency Results in Chemistry Nanotechnology Iron oxide nanoparticles Nanozyme Antibacterial agents |
| title | Peroxidase-like activity of citrate-functionalized magnetite nanoparticles augmented its antibacterial potency |
| title_full | Peroxidase-like activity of citrate-functionalized magnetite nanoparticles augmented its antibacterial potency |
| title_fullStr | Peroxidase-like activity of citrate-functionalized magnetite nanoparticles augmented its antibacterial potency |
| title_full_unstemmed | Peroxidase-like activity of citrate-functionalized magnetite nanoparticles augmented its antibacterial potency |
| title_short | Peroxidase-like activity of citrate-functionalized magnetite nanoparticles augmented its antibacterial potency |
| title_sort | peroxidase like activity of citrate functionalized magnetite nanoparticles augmented its antibacterial potency |
| topic | Nanotechnology Iron oxide nanoparticles Nanozyme Antibacterial agents |
| url | http://www.sciencedirect.com/science/article/pii/S2211715625000281 |
| work_keys_str_mv | AT haithamalmadhagi peroxidaselikeactivityofcitratefunctionalizedmagnetitenanoparticlesaugmenteditsantibacterialpotency AT abdalraouftarabishi peroxidaselikeactivityofcitratefunctionalizedmagnetitenanoparticlesaugmenteditsantibacterialpotency AT fatimaalzahrahamsho peroxidaselikeactivityofcitratefunctionalizedmagnetitenanoparticlesaugmenteditsantibacterialpotency AT zahertahan peroxidaselikeactivityofcitratefunctionalizedmagnetitenanoparticlesaugmenteditsantibacterialpotency AT lamaalchab peroxidaselikeactivityofcitratefunctionalizedmagnetitenanoparticlesaugmenteditsantibacterialpotency AT wassimabdelwahed peroxidaselikeactivityofcitratefunctionalizedmagnetitenanoparticlesaugmenteditsantibacterialpotency |