Construction of a reading frame–independent yeast two-hybrid vector system for site-specific recombinational cloning and protein interaction screening
The yeast two-hybrid (Y2H) system is a powerful method to identify protein-protein inter-actions (PPI) in vivo, requiring minimal prior information of the putative interactors. The time and effort required for each experiment can be significantly reduced if the “bait” and the “prey” proteins are clo...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2008-09-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112897 |
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