Relationships Between <i>Candida auris</i> and the Rest of the <i>Candida</i> World—Analysis of Dual-Species Biofilms and Infections
In this study, we investigated the interactions between <i>Candida auris</i> and <i>C. albicans</i>, <i>C. tropicalis</i>, <i>C. glabrata</i>, and <i>C. krusei</i> in mixed infections. Initially, these interactions were studied qualitativel...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/14/1/40 |
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| Summary: | In this study, we investigated the interactions between <i>Candida auris</i> and <i>C. albicans</i>, <i>C. tropicalis</i>, <i>C. glabrata</i>, and <i>C. krusei</i> in mixed infections. Initially, these interactions were studied qualitatively and quantitatively in dual-species biofilms formed in vitro. The MTT assays, determination of the total CFU/mL, and SEM analysis showed that <i>C. auris</i> interacted differentially with the other <i>Candida</i> spp. during the dual-species biofilm formation. Depending on the stage of the biofilm development, <i>C. auris</i> was found to be a particularly dominant species during its interaction with the <i>C. krusei</i> biofilms but significantly submissive in the <i>C. auris-C. albicans</i> biofilms. These studies were then extended to in vivo host models of experimental candidiasis. <i>G. mellonella</i> larvae were inoculated with monotypic and heterotypic suspensions of <i>Candida</i>. The survival rates and quantification of fungal cells in the hemolymph showed that the highest mortality was exhibited by larvae in the <i>C. auris-C. albicans</i> co-infection (100% mortality after 36 h). The CFU/mL values of <i>C. auris</i> from the larval hemolymph were lower in the interactive groups compared to the mono-species group. As a newly emerging species, <i>C. auris</i> persists in environments in the presence of other <i>Candida</i> species and is involved in both competitive and noncompetitive interactions with other <i>Candida</i> species during biofilm formation and development of experimental candidiasis. |
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| ISSN: | 2076-0817 |