Hydrogel Containing Biogenic Silver Nanoparticles and <i>Origanum vulgare</i> Essential Oil for Burn Wounds: Antimicrobial Efficacy Using Ex Vivo and In Vivo Methods Against Multidrug-Resistant Microorganisms

Hydrogel Containing Biogenic Silver Nanoparticles and <i>Origanum vulgare</i> Essential Oil for Burn Wounds: Antimicrobial Efficacy Using Ex Vivo and In Vivo Methods Against Multidrug-Resistant Microorganisms

<b>Background/Objectives:</b> Wounds from burns are susceptible to infections, allowing multidrug-resistant microorganisms to complicate treatments and patient recovery. This highlights the development of new strategies to control these microorganisms. This work evaluated the antibacteri...

Full description

Saved in:
Bibliographic Details
Main Authors: Angela Hitomi Kimura, Débora Dahmer, Luana Ayumi Isawa, Ana Beatriz Olivetti da Silva, Lucas Marcelino dos Santos Souza, Pedro Henrique Takata, Sara Scandorieiro, Anastácia Nikolaos Deonas, Jennifer Germiniani-Cardozo, Eliana Carolina Vespero, Marcia Regina Eches Perugini, Nilton Lincopan, Audrey Alesandra Stinghen Garcia Lonni, Gerson Nakazato, Renata Katsuko Takayama Kobayashi
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Pharmaceutics
Subjects:
<i>Galleria mellonella</i>
porcine skin
<i>Staphylococcus aureus</i>
<i>Klebsiella pneumoniae</i>
<i>Pseudomonas aeruginosa</i>
green nanotechnology
Online Access:https://www.mdpi.com/1999-4923/17/4/503
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:<b>Background/Objectives:</b> Wounds from burns are susceptible to infections, allowing multidrug-resistant microorganisms to complicate treatments and patient recovery. This highlights the development of new strategies to control these microorganisms. This work evaluated the antibacterial activity of hydrogels containing biogenic silver nanoparticles (bio-AgNP) and <i>Origanum vulgare</i> essential oil (OEO) against multidrug-resistant bacteria. <b>Methods:</b> The formulations were subjected to organoleptic, pharmacotechnical, and stability characterization and antimicrobial activity assessment by time–kill tests and alternative methods, an ex vivo model using porcine skin, and an in vivo model using <i>Galleria mellonella</i>. <b>Results:</b> All hydrogels maintained their stability after the thermal stress. The hydrogel containing bio-AgNP + OEO 1% (HAgNP + OEO1) presented bactericidal effectiveness, within 2 h, against both Gram-positive and Gram-negative multidrug-resistant bacteria in the time–kill test. For alternative testing, HAgNP + OEO1 was compared with 1% silver sulfadiazine (SS) and the base formulation. In the ex vivo test, both HAgNP + OEO1 and SS treatments showed a similar reduction in superficial washing of the burn for <i>S. aureus</i> 999, while for <i>P. aeruginosa</i>, the reduction was more expressive for SS treatment. In the burn tissue, HAgNP + OEO1 treatment was more effective against <i>S. aureus</i> 999, while for <i>P. aeruginosa</i> 1461, both formulations were similarly effective. In the <i>Galleria mellonella</i> test, survival rates after 48 h were 84% for the control group (base) and 50% for both HAgNP + OEO1 and SS treatment groups. <b>Conclusions:</b> This study demonstrates that the hydrogel combining antimicrobials is effective against multidrug-resistant microorganisms, offering a promising alternative for the treatment of infected burns.
ISSN:1999-4923

Similar Items