Development and Validation of Multiplex-PCR Assay for <i>β-Carotene hydroxylase</i> and <i>γ-Tocopherol methyl transferase</i> Genes Governing Enhanced Multivitamins in Maize for Its Application in Genomics-Assisted Breeding

Development and Validation of Multiplex-PCR Assay for <i>β-Carotene hydroxylase</i> and <i>γ-Tocopherol methyl transferase</i> Genes Governing Enhanced Multivitamins in Maize for Its Application in Genomics-Assisted Breeding

Traditional maize possesses low concentrations of provitamin-A and vitamin-E, leading to various health concerns. Mutant alleles of <i>crtRB1</i> and <i>vte4</i> that enhance β-carotene (provitamin-A) and α-tocopherol (vitamin-E), respectively, in maize kernels have been expl...

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Main Authors: Munegowda Manoj Gowda, Vignesh Muthusamy, Rashmi Chhabra, Hriipulou Duo, Saikat Pal, Nisrita Gain, Ashvinkumar Katral, Ravindra K. Kasana, Rajkumar U. Zunjare, Firoz Hossain
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Plants
Subjects:
maize
uniplex- and multiplex-PCR
cost and time
genomics-assisted breeding
biofortification
Online Access:https://www.mdpi.com/2223-7747/14/1/142
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