Peningkatan akumulasi tiamin pada kultur Escherichia coli melalui peningkatan oksigenasi dan penghilangan rpoS
Microorganisms as cell factories in producing a product are strongly influenced by their metabolism. engineering microbial metabolism by increasing coenzymes that play a role in enzyme activity. Thiamine diphosphate is one of the coenzymes that are commonly found in the metabolic pathways of microbi...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Trunojoyo Madura
2025-01-01
|
| Series: | Agrointek |
| Subjects: | |
| Online Access: | https://journal.trunojoyo.ac.id/agrointek/article/view/11110 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832584844676169728 |
|---|---|
| author | Nuraliah Rusman Prayoga Suryadarma Nisa Rachmania Mubarik |
| author_facet | Nuraliah Rusman Prayoga Suryadarma Nisa Rachmania Mubarik |
| author_sort | Nuraliah Rusman |
| collection | DOAJ |
| description | Microorganisms as cell factories in producing a product are strongly influenced by their metabolism. engineering microbial metabolism by increasing coenzymes that play a role in enzyme activity. Thiamine diphosphate is one of the coenzymes that are commonly found in the metabolic pathways of microbial cells. Increasing the thiamine formation pathway as a precursor in the thiamine metabolic pathway is a strategy in this study. Enhancing thiamine accumulation in Escherichia coli culture by inducing oxygenation level and deleting rpoS was investigated. The investigation was conducted by comparing the cultivation of Escherichia coli BW25113 (parent strain) and BW25113-∆rpoS (rpoS mutant) cultures under aerobic conditions at different shaking rotation rates of 150 and 200 rpm. The measured parameters were thiamine accumulation, dry cell weight, acetate concentration and glucose consumption of the cultures. Inducing oxygenation level of parent strain cultures through increasing shaking rotation rate from 150 to 200 rpm induced 40 % thiamine accumulation. Meanwhile, deletion of rpoS increased 9,5 % thiamine accumulation compared to parent strain culture |
| format | Article |
| id | doaj-art-1ae225b6484d4f578e3895ad1475a8df |
| institution | Kabale University |
| issn | 1907-8056 2527-5410 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Universitas Trunojoyo Madura |
| record_format | Article |
| series | Agrointek |
| spelling | doaj-art-1ae225b6484d4f578e3895ad1475a8df2025-01-27T10:43:11ZengUniversitas Trunojoyo MaduraAgrointek1907-80562527-54102025-01-01191717910.21107/agrointek.v19i1.111108901Peningkatan akumulasi tiamin pada kultur Escherichia coli melalui peningkatan oksigenasi dan penghilangan rpoSNuraliah Rusman0Prayoga Suryadarma1Nisa Rachmania Mubarik2Program Studi Bioteknologi, Sekolah Pascasarjana, Institut Pertanian BogorProgram Studi Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Institut Pertanian BogorDepartemen Biologi, Fakultas MIPA, Institut Pertanian BogorMicroorganisms as cell factories in producing a product are strongly influenced by their metabolism. engineering microbial metabolism by increasing coenzymes that play a role in enzyme activity. Thiamine diphosphate is one of the coenzymes that are commonly found in the metabolic pathways of microbial cells. Increasing the thiamine formation pathway as a precursor in the thiamine metabolic pathway is a strategy in this study. Enhancing thiamine accumulation in Escherichia coli culture by inducing oxygenation level and deleting rpoS was investigated. The investigation was conducted by comparing the cultivation of Escherichia coli BW25113 (parent strain) and BW25113-∆rpoS (rpoS mutant) cultures under aerobic conditions at different shaking rotation rates of 150 and 200 rpm. The measured parameters were thiamine accumulation, dry cell weight, acetate concentration and glucose consumption of the cultures. Inducing oxygenation level of parent strain cultures through increasing shaking rotation rate from 150 to 200 rpm induced 40 % thiamine accumulation. Meanwhile, deletion of rpoS increased 9,5 % thiamine accumulation compared to parent strain culturehttps://journal.trunojoyo.ac.id/agrointek/article/view/11110escherichia colithiamine accumulationdeletion rposoxygenation treatment |
| spellingShingle | Nuraliah Rusman Prayoga Suryadarma Nisa Rachmania Mubarik Peningkatan akumulasi tiamin pada kultur Escherichia coli melalui peningkatan oksigenasi dan penghilangan rpoS Agrointek escherichia coli thiamine accumulation deletion rpos oxygenation treatment |
| title | Peningkatan akumulasi tiamin pada kultur Escherichia coli melalui peningkatan oksigenasi dan penghilangan rpoS |
| title_full | Peningkatan akumulasi tiamin pada kultur Escherichia coli melalui peningkatan oksigenasi dan penghilangan rpoS |
| title_fullStr | Peningkatan akumulasi tiamin pada kultur Escherichia coli melalui peningkatan oksigenasi dan penghilangan rpoS |
| title_full_unstemmed | Peningkatan akumulasi tiamin pada kultur Escherichia coli melalui peningkatan oksigenasi dan penghilangan rpoS |
| title_short | Peningkatan akumulasi tiamin pada kultur Escherichia coli melalui peningkatan oksigenasi dan penghilangan rpoS |
| title_sort | peningkatan akumulasi tiamin pada kultur escherichia coli melalui peningkatan oksigenasi dan penghilangan rpos |
| topic | escherichia coli thiamine accumulation deletion rpos oxygenation treatment |
| url | https://journal.trunojoyo.ac.id/agrointek/article/view/11110 |
| work_keys_str_mv | AT nuraliahrusman peningkatanakumulasitiaminpadakulturescherichiacolimelaluipeningkatanoksigenasidanpenghilanganrpos AT prayogasuryadarma peningkatanakumulasitiaminpadakulturescherichiacolimelaluipeningkatanoksigenasidanpenghilanganrpos AT nisarachmaniamubarik peningkatanakumulasitiaminpadakulturescherichiacolimelaluipeningkatanoksigenasidanpenghilanganrpos |