Metabolomic Analysis of Key Regulatory Metabolites in the Urine of Flavivirus-Infected Mice
Objective. Dengue virus (DENV), Japanese encephalitis virus (JEV), and Zika virus (ZIKV) are several important flaviviruses, and infections caused by these flaviviruses remain worldwide health problems. Different flaviviruses exhibit different biological characteristics and pathogenicity. Metabolomi...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Tropical Medicine |
| Online Access: | http://dx.doi.org/10.1155/2022/4663735 |
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| _version_ | 1832554953704472576 |
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| author | Xiaoyan Zheng Ran Wang |
| author_facet | Xiaoyan Zheng Ran Wang |
| author_sort | Xiaoyan Zheng |
| collection | DOAJ |
| description | Objective. Dengue virus (DENV), Japanese encephalitis virus (JEV), and Zika virus (ZIKV) are several important flaviviruses, and infections caused by these flaviviruses remain worldwide health problems. Different flaviviruses exhibit different biological characteristics and pathogenicity. Metabolomics is an emerging research perspective to uncover and observe the pathogenesis of certain infections. Methods. To improve the understanding of the specific metabolic changes that occur during infection with different flaviviruses, considering the principle of noninvasive sampling, this article describes our comprehensive analysis of metabolites in urine samples from the three kinds of flavivirus-infected mice using a liquid chromatography tandem mass spectrometry method to better understand their infection mechanisms. Results. The urine of DENV-, JEV-, and ZIKV-infected mice had 68, 64, and 47 different differential metabolites, respectively, compared with the urine of control mice. Among the metabolic pathways designed by these metabolites, ABC transporters, arginine and proline metabolism, and regulation of lipolysis play an important role. Furthermore, we predicted and fitted potential relationships between metabolites and pathways. Conclusions. These virus-specific altered metabolites may be associated with their unique biological properties and pathogenicity. The metabolomic analysis of urine is very important for the analysis of flavivirus infection. |
| format | Article |
| id | doaj-art-1ab28a8baf5d446bb3f00d8c186b5636 |
| institution | Kabale University |
| issn | 1687-9694 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Tropical Medicine |
| spelling | doaj-art-1ab28a8baf5d446bb3f00d8c186b56362025-02-03T05:49:58ZengWileyJournal of Tropical Medicine1687-96942022-01-01202210.1155/2022/4663735Metabolomic Analysis of Key Regulatory Metabolites in the Urine of Flavivirus-Infected MiceXiaoyan Zheng0Ran Wang1Beijing Institute of Tropical MedicineBeijing Key Laboratory of Pediatric Respiratory Infection DiseasesObjective. Dengue virus (DENV), Japanese encephalitis virus (JEV), and Zika virus (ZIKV) are several important flaviviruses, and infections caused by these flaviviruses remain worldwide health problems. Different flaviviruses exhibit different biological characteristics and pathogenicity. Metabolomics is an emerging research perspective to uncover and observe the pathogenesis of certain infections. Methods. To improve the understanding of the specific metabolic changes that occur during infection with different flaviviruses, considering the principle of noninvasive sampling, this article describes our comprehensive analysis of metabolites in urine samples from the three kinds of flavivirus-infected mice using a liquid chromatography tandem mass spectrometry method to better understand their infection mechanisms. Results. The urine of DENV-, JEV-, and ZIKV-infected mice had 68, 64, and 47 different differential metabolites, respectively, compared with the urine of control mice. Among the metabolic pathways designed by these metabolites, ABC transporters, arginine and proline metabolism, and regulation of lipolysis play an important role. Furthermore, we predicted and fitted potential relationships between metabolites and pathways. Conclusions. These virus-specific altered metabolites may be associated with their unique biological properties and pathogenicity. The metabolomic analysis of urine is very important for the analysis of flavivirus infection.http://dx.doi.org/10.1155/2022/4663735 |
| spellingShingle | Xiaoyan Zheng Ran Wang Metabolomic Analysis of Key Regulatory Metabolites in the Urine of Flavivirus-Infected Mice Journal of Tropical Medicine |
| title | Metabolomic Analysis of Key Regulatory Metabolites in the Urine of Flavivirus-Infected Mice |
| title_full | Metabolomic Analysis of Key Regulatory Metabolites in the Urine of Flavivirus-Infected Mice |
| title_fullStr | Metabolomic Analysis of Key Regulatory Metabolites in the Urine of Flavivirus-Infected Mice |
| title_full_unstemmed | Metabolomic Analysis of Key Regulatory Metabolites in the Urine of Flavivirus-Infected Mice |
| title_short | Metabolomic Analysis of Key Regulatory Metabolites in the Urine of Flavivirus-Infected Mice |
| title_sort | metabolomic analysis of key regulatory metabolites in the urine of flavivirus infected mice |
| url | http://dx.doi.org/10.1155/2022/4663735 |
| work_keys_str_mv | AT xiaoyanzheng metabolomicanalysisofkeyregulatorymetabolitesintheurineofflavivirusinfectedmice AT ranwang metabolomicanalysisofkeyregulatorymetabolitesintheurineofflavivirusinfectedmice |