Nucleic Acid-based Identification of Major Honeybee (Apis mellifera L.) Viruses in the Jammu Region of India
The present investigation was conducted from 2019 to 2020 to study the incidence pattern and molecular diagnosis of major honeybee (Apis mellifera L.) viral diseases in different areas of Jammu region of Jammu and Kashmir (UT). Using the RT-PCR technology, research was done to confirm that A. melli...
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Universidade Estadual de Feira de Santana
2024-11-01
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| Series: | Sociobiology |
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| Online Access: | https://ojs3.uefs.br/index.php/sociobiology/article/view/10394 |
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| author | Ram Narayan Sharma Ram Devinder Sharma Biswajit Brahma Pradeep Kumar Kumawat Sharad Kumar Meena |
| author_facet | Ram Narayan Sharma Ram Devinder Sharma Biswajit Brahma Pradeep Kumar Kumawat Sharad Kumar Meena |
| author_sort | Ram Narayan Sharma Ram |
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The present investigation was conducted from 2019 to 2020 to study the incidence pattern and molecular diagnosis of major honeybee (Apis mellifera L.) viral diseases in different areas of Jammu region of Jammu and Kashmir (UT). Using the RT-PCR technology, research was done to confirm that A. mellifera is infected by the Israeli acute paralysis virus (IAPV), the Thai sacbrood virus (TSBV), the Kashmir bee virus (KBV), and sacbrood virus (SBV). Particular primers for distinct viruses verified the existence of the virus in chosen samples from various areas, including Jammu, Samba, Ramban, and Udhampur. The samples taken from all four districts were found to contain illness. Clear and distinct bands with molecular sizes of roughly 110, 122, and 119 bp for IAPV, KBV, and SBV were formed by primer pairs of various viruses. The four districts’ samples contained no evidence of the Thai sacbrood virus. The incidence range of Israeli acute paralysis disease was 8.33 to 21.42 %. The incidence range for Kashmir bee disease was 5.71 to 28.33 %. The incidence range for sacbrood disease was 8.0–18.55%. In the apiary at SKUAST-J, Chatha, the incidence ranges for IAPV, KBV, and SBV diseases were 0 to 6.25, 0 to 4.16, and 0 to 9.43 %, respectively.
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| format | Article |
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| institution | Kabale University |
| issn | 0361-6525 2447-8067 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Universidade Estadual de Feira de Santana |
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| spelling | doaj-art-19e2d1e201b641eb9aa2ed8be3272e2e2025-02-03T11:44:40ZengUniversidade Estadual de Feira de SantanaSociobiology0361-65252447-80672024-11-0171410.13102/sociobiology.v71i4.10394Nucleic Acid-based Identification of Major Honeybee (Apis mellifera L.) Viruses in the Jammu Region of IndiaRam Narayan Sharma Ram0Devinder Sharma1Biswajit Brahma2Pradeep Kumar Kumawat3Sharad Kumar Meena4Division of Entomology, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, IndiaDivision of Entomology, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, IndiaDivision of LPM, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, Indiaivision of Entomology, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, IndiaDepartment of Entomology, Vasantrao Naik Marthawada Krishi Vidhyapeeth, Parbhani, Maharashtra, India The present investigation was conducted from 2019 to 2020 to study the incidence pattern and molecular diagnosis of major honeybee (Apis mellifera L.) viral diseases in different areas of Jammu region of Jammu and Kashmir (UT). Using the RT-PCR technology, research was done to confirm that A. mellifera is infected by the Israeli acute paralysis virus (IAPV), the Thai sacbrood virus (TSBV), the Kashmir bee virus (KBV), and sacbrood virus (SBV). Particular primers for distinct viruses verified the existence of the virus in chosen samples from various areas, including Jammu, Samba, Ramban, and Udhampur. The samples taken from all four districts were found to contain illness. Clear and distinct bands with molecular sizes of roughly 110, 122, and 119 bp for IAPV, KBV, and SBV were formed by primer pairs of various viruses. The four districts’ samples contained no evidence of the Thai sacbrood virus. The incidence range of Israeli acute paralysis disease was 8.33 to 21.42 %. The incidence range for Kashmir bee disease was 5.71 to 28.33 %. The incidence range for sacbrood disease was 8.0–18.55%. In the apiary at SKUAST-J, Chatha, the incidence ranges for IAPV, KBV, and SBV diseases were 0 to 6.25, 0 to 4.16, and 0 to 9.43 %, respectively. https://ojs3.uefs.br/index.php/sociobiology/article/view/10394A. MelliferaincidenceIAPVKBVSBVTSBV |
| spellingShingle | Ram Narayan Sharma Ram Devinder Sharma Biswajit Brahma Pradeep Kumar Kumawat Sharad Kumar Meena Nucleic Acid-based Identification of Major Honeybee (Apis mellifera L.) Viruses in the Jammu Region of India Sociobiology A. Mellifera incidence IAPV KBV SBV TSBV |
| title | Nucleic Acid-based Identification of Major Honeybee (Apis mellifera L.) Viruses in the Jammu Region of India |
| title_full | Nucleic Acid-based Identification of Major Honeybee (Apis mellifera L.) Viruses in the Jammu Region of India |
| title_fullStr | Nucleic Acid-based Identification of Major Honeybee (Apis mellifera L.) Viruses in the Jammu Region of India |
| title_full_unstemmed | Nucleic Acid-based Identification of Major Honeybee (Apis mellifera L.) Viruses in the Jammu Region of India |
| title_short | Nucleic Acid-based Identification of Major Honeybee (Apis mellifera L.) Viruses in the Jammu Region of India |
| title_sort | nucleic acid based identification of major honeybee apis mellifera l viruses in the jammu region of india |
| topic | A. Mellifera incidence IAPV KBV SBV TSBV |
| url | https://ojs3.uefs.br/index.php/sociobiology/article/view/10394 |
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